6 research outputs found
screening for adrenocortical and thyroid peroxidase antibodies to look for underlying autoimmune etiologies in women under 35 with idiopathic diminished ovarian reserve
Noninvasive prenatal diagnosis experience in the Cukurova Region of Southern Turkey: detecting paternal mutations of sickle cell anemia and beta-thalassemia in cell-free fetal DNA using high-resolution melting analysis
WOS: 000326106700008PubMed ID: 23836351ObjectiveThis study used a high-resolution melting (HRM) technique to detect paternal mutations for the noninvasive prenatal diagnosis (NIPD) of -thalassemia and sickle cell anemia (HbS). We also determined the levels of cell-free fetal DNA and total cell-free DNA. MethodsWe used the HRM technique for fetal genotyping of paternal mutations in maternal plasma from 32 pregnancies at risk of -thalassemia and 57 pregnancies at risk of HbS. The DNA levels in maternal plasma were measured using real-time quantitative PCR. Multiples of the median (MoM) values were calculated in women at risk for -thalassemia or HbS. ResultsTwenty-two paternal mutations were detected in 89 pregnant women. Although we were successfully able to detect the paternal -thalassemia mutations, the mutant HbS fetuses could not be distinguished from maternal background in the early weeks of pregnancy. The detection of DYS14 in male fetuses was 100%. The MoM values of women at high risk of having HbS-affected fetuses were higher than those for the other groups. ConclusionHigh-resolution melting is a useful method for NIPD of -thalassemias by detecting paternal mutations in the maternal plasma. Cell-free fetal DNA quantification and MoM values were not informative for HbS or -thalassemias in early pregnancy. (c) 2013 John Wiley & Sons, Ltd.Cukurova University Academic Research Projects UnitCukurova UniversityThis study was supported by the Cukurova University Academic Research Projects Unit
Sexual functions and quality of life of women over 50 years with urinary incontinence, lower urinary tract symptoms and/or pelvic organ prolapse.
Juvenile granulosa cell tumor of the ovary as a cause of isosexual precocious puberty in a 3-year-old girl
An Analysis of C-Reactive Protein, Procalcitonin, and D-Dimer in Pre-Eclamptic Patients
PubMedID: 22783989Problem: The aim of this study was to evaluate serum procalcitonin (PCT), C-reactive protein (CRP), and plasma D-Dimer levels in mild and severe pre-eclampsia. Method of study: Serum PCT, CRP, and D-Dimer levels were analyzed in 64 cases with pre-eclampsia as the study group and 33 healthy pregnant women in the third trimester as the control group. Pre-eclamptic group consisted of mild (n = 31) and severe pre-eclamptic subgroup (n = 33). Laboratory results were compared between the groups and diagnostic usefulness of these parameters were evaluated. Results: PCT, CRP, and D-Dimer levels were significantly higher in study group than the control group (P = 0.001). PCT, CRP, and D-Dimer were significantly higher in the patients with severe pre-eclampsia than mild pre-eclampsia. There were significant positive correlations between these markers and mean arterial pressure (MAP). Logistic regression analysis using the control and pre-eclampsia group showed that higher PCT (OR, 15.68; 95%-CI, 3.15-78.10), CRP (OR, 14.29; 95%-CI, 3.08-66.34), and D-Dimer levels (OR, 4.97; 95%-CI, 1.22-20.29) were found to be risk factors significantly associated with pre-eclampsia. Conclusions: This study results confirm that evidence of a possible exaggerated systemic inflammatory response in pre-eclampsia especially in severe pre-eclampsia. © 2012 John Wiley & Sons A/S