Jamming coverage in competitive random sequential adsorption of binary mixture

We propose a generalized car parking problem where cars of two different sizes are sequentially parked on a line with a given probability $q$. The free parameter $q$ interpolates between the classical car parking problem of only one car size and the competitive random sequential adsorption (CRSA) of a binary mixture. We give an exact solution to the CRSA rate equations and find that the final coverage, the jamming limit, of the line is always larger for a binary mixture than for the uni-sized case. The analytical results are in good agreement with our direct numerical simulations of the problem.Comment: 4 pages 2-column RevTeX, Four figures, (there was an error in the previous version. We replaced it (including figures) with corrected and improved version that lead to new results and conclusions

Macrophage subpopulation identity in Drosophila is modulated by apoptotic cell clearance and related signalling pathways

In Drosophila blood, plasmatocytes of the haemocyte lineage represent the functional equivalent of vertebrate macrophages and have become an established in vivo model with which to study macrophage function and behaviour. However, the use of plasmatocytes as a macrophage model has been limited by a historical perspective that plasmatocytes represent a homogenous population of cells, in contrast to the high levels of heterogeneity of vertebrate macrophages. Recently, a number of groups have reported transcriptomic approaches which suggest the existence of plasmatocyte heterogeneity, while we identified enhancer elements that identify subpopulations of plasmatocytes which exhibit potentially pro-inflammatory behaviours, suggesting conservation of plasmatocyte heterogeneity in Drosophila. These plasmatocyte subpopulations exhibit enhanced responses to wounds and decreased rates of efferocytosis when compared to the overall plasmatocyte population. Interestingly, increasing the phagocytic requirement placed upon plasmatocytes is sufficient to decrease the size of these plasmatocyte subpopulations in the embryo. However, the mechanistic basis for this response was unclear. Here, we examine how plasmatocyte subpopulations are modulated by apoptotic cell clearance (efferocytosis) demands and associated signalling pathways. We show that loss of the phosphatidylserine receptor Simu prevents an increased phagocytic burden from modulating specific subpopulation cells, while blocking other apoptotic cell receptors revealed no such rescue. This suggests that Simu-dependent efferocytosis is specifically involved in determining fate of particular subpopulations. Supportive of our original finding, mutations in amo (the Drosophila homolog of PKD2), a calcium-permeable channel which operates downstream of Simu, phenocopy simu mutants. Furthermore, we show that Amo is involved in the acidification of the apoptotic cell-containing phagosomes, suggesting that this reduction in pH may be associated with macrophage reprogramming. Additionally, our results also identify Ecdysone receptor signalling, a pathway related to control of cell death during developmental transitions, as a controller of plasmatocyte subpopulation identity. Overall, these results identify fundamental pathways involved in the specification of plasmatocyte subpopulations and so further validate Drosophila plasmatocytes as a heterogeneous population of macrophage-like cells within this important developmental and immune model

The epidermal growth factor ligand Spitz modulates macrophage efferocytosis, wound responses and migration dynamics during Drosophila embryogenesis

How multifunctional cells such as macrophages interpret the different cues within their environment and undertake an appropriate response is a key question in developmental biology. Understanding how cues are prioritized is critical to answering this – both the clearance of apoptotic cells (efferocytosis) and the migration toward damaged tissue is dependent on macrophages being able to interpret and prioritize multiple chemoattractants, polarize, and then undertake an appropriate migratory response. Here, we investigate the role of Spitz, the cardinal Drosophila epidermal growth factor (EGF) ligand, in regulation of macrophage behavior in the developing fly embryo, using activated variants with differential diffusion properties. Our results show that misexpression of activated Spitz can impact macrophage polarity and lead to clustering of cells in a variant-specific manner, when expressed either in macrophages or the developing fly heart. Spitz can also alter macrophage distribution and perturb apoptotic cell clearance undertaken by these phagocytic cells without affecting the overall levels of apoptosis within the embryo. Expression of active Spitz, but not a membrane-bound variant, can also increase macrophage migration speeds and impair their inflammatory responses to injury. The fact that the presence of Spitz specifically undermines the recruitment of more distal cells to wound sites suggests that Spitz desensitizes macrophages to wounds or is able to compete for their attention where wound signals are weaker. Taken together these results suggest this molecule regulates macrophage migration and their ability to dispose of apoptotic cells. This work identifies a novel regulator of Drosophila macrophage function and provides insights into signal prioritization and integration in vivo. Given the importance of apoptotic cell clearance and inflammation in human disease, this work may help us to understand the role EGF ligands play in immune cell recruitment during development and at sites of disease pathology

Simu-dependent clearance of dying cells regulates macrophage function and inflammation resolution

Macrophages encounter and clear apoptotic cells during normal development and homeostasis, including at numerous sites of pathology. Clearance of apoptotic cells has been intensively studied, but the effects of macrophage–apoptotic cell interactions on macrophage behaviour are poorly understood. Using Drosophila embryos, we have exploited the ease of manipulating cell death and apoptotic cell clearance in this model to identify that the loss of the apoptotic cell clearance receptor Six-microns-under (Simu) leads to perturbation of macrophage migration and inflammatory responses via pathological levels of apoptotic cells. Removal of apoptosis ameliorates these phenotypes, while acute induction of apoptosis phenocopies these defects and reveals that phagocytosis of apoptotic cells is not necessary for their anti-inflammatory action. Furthermore, Simu is necessary for clearance of necrotic debris and retention of macrophages at wounds. Thus, Simu is a general detector of damaged self and represents a novel molecular player regulating macrophages during resolution of inflammation

Multi-Stakeholder Initiatives and Decarbonization in the European Food Supply Chain

The European Green Deal is a political milestone aiming to promote a carbon-neutral economy in the European Union. Decarbonizing the complex food sector requires the unified interaction among effective climate policies, economic instruments,and initiatives involving multiple stakeholders. Despite increasing efforts to highlight the importance of innovations and finance to achieve sustainable food supply chains (FSC), comprehensive information about related opportunities and barriers to mitigating emissions in the food sector is still under-explored. To cover this gap, this paper applies an existing industrial policy framework under the lens of the EU FSC to identify potential strategies that should help achieve the needed financial means and innovation actions, as well as to gauge political alignment across FSC stages. Methodologically, the pillars proposed in the framework are linked to multi-stakeholders' initiatives engaged in achieving net-zero emissions. The paper highlights three main implications of the identified interlinkages. First, political directionality related to the food sector should be more comprehensively tailored to account for the specificities of all stages of the FSC. Second, research and development projects shall likewise cover all stages,instead of emphasizing only food production and agricultural systems. Finally, multiple stakeholders are crucial as promoters of technology and innovation towards a green economy. Nevertheless, initiatives should be integrated into political discussions in order to promote civil awareness, sustainable food and services demand, aligned to political guidelines

Strings on pp-waves and Hadrons in (softly broken) N=1 gauge theories

We study the Penrose limit of Type IIB duals of softly broken N=1 SU(N) gauge theories in four dimensions, obtained as deformations of the Maldacena-Nunez and Klebanov-Strassler backgrounds. We extract the string spectrum on the resulting pp-wave backgrounds and discuss some properties of the conjectured dual gauge theory hadrons, the so called "Annulons". The string zero-point energy on the light-cone is nontrivial, due to the loss of linearly realized worldsheet supersymmetry, and negative, even in the unbroken supersymmetric case. This causes the appearance of non-perturbative corrections to the hadronic mass spectrum. We briefly discuss the thermodynamic behavior of these string models, calculating the corresponding Hagedorn temperatures.Comment: 20 page

Stability of the non-extremal enhancon solution I: perturbation equations

We consider the stability of the two branches of non-extremal enhancon solutions. We argue that one would expect a transition between the two branches at some value of the non-extremality, which should manifest itself in some instability. We study small perturbations of these solutions, constructing a sufficiently general ansatz for linearised perturbations of the non-extremal solutions, and show that the linearised equations are consistent. We show that the simplest kind of perturbation does not lead to any instability. We reduce the problem of studying the more general spherically symmetric perturbation to solving a set of three coupled second-order differential equations.Comment: 20 pages, 1 figure, references added, typos fixed, version to appear in PR

Non-supersymmetric deformations of the dual of a confining gauge theory

We introduce a computational technique for studying non-supersymmetric deformations of domain wall solutions of interest in AdS/CFT. We focus on the Klebanov-Strassler solution, which is dual to a confining gauge theory. From an analysis of asymptotics we find that there are three deformations that leave the ten-dimensional supergravity solution regular and preserve the global bosonic symmetries of the supersymmetric solution. Also, we show that there are no regular near-extremal deformations preserving the global symmetries, as one might expect from the existence of a gap in the gauge theory.Comment: 18 pages, latex, published as JHEP 0305 (2003) 03

Identification of functionally distinct macrophage subpopulations in Drosophila

Vertebrate macrophages are a highly heterogeneous cell population, but while Drosophila blood is dominated by a macrophage-like lineage (plasmatocytes), until very recently these cells were considered to represent a homogeneous population. Here, we present our identification of enhancer elements labelling plasmatocyte subpopulations, which vary in abundance across development. These subpopulations exhibit functional differences compared to the overall population, including more potent injury responses and differential localisation and dynamics in pupae and adults. Our enhancer analysis identified candidate genes regulating plasmatocyte behaviour: pan-plasmatocyte expression of one such gene (Calnexin14D) improves wound responses, causing the overall population to resemble more closely the subpopulation marked by the Calnexin14D-associated enhancer. Finally, we show that exposure to increased levels of apoptotic cell death modulates subpopulation cell numbers. Taken together this demonstrates macrophage heterogeneity in Drosophila, identifies mechanisms involved in subpopulation specification and function and facilitates the use of Drosophila to study macrophage heterogeneity in vivo

Glueballs, symmetry breaking and axionic strings in non-supersymmetric deformations of the Klebanov-Strassler background

We obtain an analytic solution for an axionic non-supersymmetric deformation of the warped deformed conifold. This allows us to study D-strings in the infrared limit of non-supersymmetric deformations of the Klebanov-Strassler background. They are interpreted as axionic strings in the dual field theory. Following the arguments of [hep-th/0405282], the axion is a massless pseudo-scalar glueball which is present in the supergravity fluctuation spectrum and it is interpreted as the Goldstone boson of the spontaneously broken U(1) baryon number symmetry, being the gauge theory on the baryonic branch. Besides, we briefly discuss about the Pando Zayas-Tseytlin solution where the SU(2) \times SU(2) global symmetry is spontaneously broken. This background has been conjectured to be on the mesonic branch of the gauge theory.Comment: 30 pages; V2: minor corrections; V3: section 3 corrected and misprints corrected to match version published in JHE