Toxicological Effects of Three Polybromodiphenyl Ethers (BDE-47, BDE-99 and BDE-154) on Growth of Marine Algae Isochrysis galbana

Polybrominated diphenyl ethers (PBDEs) are highly persistent anthropogenic contaminants found in trace amounts in many environmental compartments far from their source areas, posing a risk to aquatic ecosystems. Our objective was to determine the relative toxicities of three BDEs, BDE-47, BDE- 99 and BDE-154 on marine phytoplankton algae Isochrysis galbana. For a highly sensitive endpoint: the 72-h inhibition of autotrophic growth rate was calculated according to standards methods. Actual PBDE concentration was measured by GC-MS and toxicity parameters were calculated on the basis of time-weighted mean actual concentrations. No observable effect concentration (NOEC) values were 2.53 μg L−1 for BDE-47, 3.48 μg L−1 for BDE-99 and 12.3 μg L−1 for BDE-154, and LOEC values were 5.06, 6.96 and 24.60 μg L−1 for BDE-47, BDE-99 and BDE- 154, respectively. The calculated IC10 (the concentration inhibiting growth rate by 10 %) corresponded to 9.3, 12.78 and 54.6 μg L−1 for BDE-47, BDE-99 and BDE- 154, respectively. The 50 % inhibitions of growth rate (IC50) values were: 25.7 μg L−1 BDE-47, 30.0 μg L−1 BDE-99 and 243.7 μg L−1 BDE-154. Therefore, the acute toxicity of PBDEs decreases as the degree of bromination increases, the order of toxicity is BDE-47>BDE-99> BDE-154. Significant (p<0.05) adverse effects were observed for all compounds at concentrations >15 μg L−1. Our results indicated that under laboratory conditions PBDEs inhibited the growth of marine phytoplankton at concentrations near 10 μg L−1. However, further work is required to investigate long-term effects in these and other aquatic organisms.MAE-PCI MCINN CTM2009-10908Versión del editor1,748