Lethality - day 7 - 87 compounds

The data is each planarian's status at day 7. Alive planarian was marked as "0". Dead planarian was marked as "1". Suicide planarian was marked as "10"

Eye regeneration - day 7 - 87 compounds

The data is regenerated eyes of regenerating tails at day 7. Normal eye regeneration with 2 eyes regenerated was marked as "1". Abnormal eye regeneration with 0/1/>2 eyes regenerated was marked as "0". Indeterminate planarian (i.e. when the worm was flipped over) was marked as "-1". Dead/suicide planarian was marked as "NaN". Both Indeterminate and dead/suicide planarians were considered as non-analyzable planarians

Description for raw data files

A summary description for the 9 excel files of raw data

Thermotaxis - day 12 - 87 compounds

The data is quantification of planarian's response to temperature gradient in thermotaxis assay at day 12, which is the fraction of time the planarian spent in the cold area during the last minutes of the assay. Non analyzable planarian was marked as "NaN"

Phototaxis - day 12 - 87 compounds

The data is the binary presence/absence of a response to light in phototaxis assay at day 12. Planarian having reacted to the light stimulus was marked as "1". Planarian with no reaction to light was marked as "0". Non-analyzable planarian was marked as "NaN"

Lethality - day 12 - 87 compounds

The data is each planarian's status at day 12. Alive planarian was marked as "0". Dead planarian was marked as "1". Suicide planarian was marked as "10"

Scrunching - day 12 - 87 compounds

The data is the binary presence/absence of scrunching behavior during the scrunching assay. The scrunching planarian was marked as "1". The non-scrunching planarian was marked as "0". The non-analyzable planarian was marked as "NaN"

Unstimulated behavior - day 12 - 87 compounds

The data is quantification of planarian's behavior in unstimulated assay at day 7. The speed data is the mean speed of each planarian. The resting data is the fraction of time planarian spent resting in the unstimulated assay. Non-analyzable planarians, including dead/suicide planarians, were marked as "NaN"

<i>Tryptophan hydroxylase</i> Is Required for Eye Melanogenesis in the Planarian <i>Schmidtea mediterranea</i>

<div><p>Melanins are ubiquitous and biologically important pigments, yet the molecular mechanisms that regulate their synthesis and biochemical composition are not fully understood. Here we present a study that supports a role for serotonin in melanin synthesis in the planarian <i>Schmidtea mediterranea</i>. We characterize the tryptophan hydroxylase (<i>tph</i>) gene, which encodes the rate-limiting enzyme in serotonin synthesis, and demonstrate by RNA interference that <i>tph</i> is essential for melanin production in the pigment cups of the planarian photoreceptors. We exploit this phenotype to investigate the biological function of pigment cups using a quantitative light-avoidance behavioral assay. Planarians lacking eye pigment remain phototactic, indicating that eye pigmentation is not essential for light avoidance in <i>S</i>. <i>mediterranea</i>, though it improves the efficiency of the photophobic response. Finally, we show that the eye pigmentation defect observed in <i>tph</i> knockdown animals can be rescued by injection of either the product of TPH, 5-hydroxytryptophan (5-HTP), or serotonin. Together, these results highlight a role for serotonin in melanogenesis, perhaps as a regulatory signal or as a pigment substrate. To our knowledge, this is the first example of this relationship to be reported outside of mammalian systems.</p></div

Pigment cup and photoreceptors remain intact in <i>tph(RNAi)</i> animals.

<p>(A-B) The planarian visual system. Photoreceptors (magenta) are visualized by immunofluorescence with VC-1 antibody against arrestin, and pigment cup cells (green) are visualized by fluorescent <i>in situ</i> hybridization of <i>tyrosinase (tyr)</i>. (A) depicts a maximum intensity confocal projection, whereas (B) represents a single confocal section. (C) By 10 days post-amputation, control and <i>tph(RNAi)</i> animals regenerate both photoreceptors and pigment cup cells, as indicated by VC-1 staining and <i>tyr</i> mRNA expression. Scale bars 20 μm.</p