A chemotaxonomic investigation on Vitis vinifera1. Within-cultivar population analysis

The extent of variability for storage protein subunits as well as the isozymes of AcP, EST and PGM was evaluated by isoelectric focusing on a large number of individual self-pollinated seeds from cvs Chardonnay, Sangiovese and Traminer. Extracts from 35 randomly selected kernels gave reproducible protein patterns and may thus be taken as representative of the average genetic make-up in a given biotype

A chemotaxonomic investigation on Vitis vinifera L. II. Comparison among ssp. sativa traditional cultivars and wild biotypes of ssp. silvestris from various Italian regions

An extensive screening on regional representatives of Vitis vinifera ssp. sativa and silvestris was carried out to look for relationships and differences between different taxa. Total proteins in the pH range 4.0-5.5, and the enzymes AcP, ADH, EST, G-6-PDH, MDH, PGM and POD were recorded. Only patterns of storage protein subunits, AcP, EST and G-6-PDH were taxonomically informative. Dendrograms were computerized on the basis of presence/absence of individual bands; these always distinguish at different levels in homogeneous groups between ssp. sativa and ssp. silvestris. The acidic subunits showed a dichotomy from the first branching of the cladogram. These observations are a demonstration that neither hypotheses of direct or indirect origin of the Italian ssp. sativa from Italian ssp. silvestris via domestication is tenanble. The authors suggest that, the morphological, the ecological and the biochemical differences between the two taxa support the hypothesis that V. sativa and V. silvestris should be regarded as two separate taxa that have had reciprocal interactions such a long period of time that a precise location of origin is no longer possible

Comparison of Early and Long-Term Outcomes After Transcatheter Aortic Valve Implantation in Patients with New York Heart Association Functional Class IV to those in Class III and Less

Our aim was to investigate the impact of a baseline New York Heart Association (NYHA) class IV on clinical outcomes of a large real-world population who underwent transcatheter aortic valve implantation (TAVI). The primary end points were all-cause mortality, cardiovascular mortality, and re-hospitalization, evaluated at the longest available follow-up and by means of a 3-month landmark analysis. The secondary end points were: change in NYHA class, left ventricular ejection fraction, pulmonary pressure and mitral regurgitation. Out of 2,467 patients, 271 (11%) had a NYHA functional class IV at the admission. The latter had higher Society of Thoracic Surgeons (STS) score (9.2% vs 5.5%; p < 0.001) compared to NYHA ≤ III patients, owing to more comorbidities (prior myocardial infarction, severe long-term kidney disease, atrial fibrillation, left ventricular dysfunction, significant mitral regurgitation, pulmonary hypertension). Device success was similar between the two groups (93.7% vs 94.5%; p = 0.583). At a median follow-up of 15 months (interquartile range 4 to 36 months) a lower freedom from primary end points was observed among NYHA IV versus NYHA ≤ III group (survival from all-cause death: 52% vs 58.4%; p = 0.002; survival from cardiovascular death: 72.5% vs 76.5%; p = 0.091; freedom from re-hospitalization: 81.5% vs 85.4%; p = 0.038). However, after adjustment for baseline imbalance, NYHA IV did not influence the relative risk of long-term primary end points. A 3-month landmark analysis showed that NYHA IV independently predicted 3-month all-cause and cardiovascular mortality (hazard ratio: 1.77; 95% CI [1.10 to 2.83]; p = 0.018 and hazard ratio: 1.64; 95% CI [1.03 to 2.59]; p = 0.036, respectively). Instead, after 3-month follow-up NYHA IV did not affect the risk of primary end points. A significant improvement of the secondary end points was noted in both NYHA IV and NYHA ≤≤ III groups. In conclusion, the presence of NYHA class IV in TAVI candidates was associated to a significant increased risk of mortality within 3 months. Patients with baseline NYHA IV who survived at 3 months had a long-term outcome comparable to that of other subjects. Left ventricular systolic function, pulmonary pressure, and mitral insufficiency significantly improved after TAVI regardless of baseline NYHA class IV

Erratum

**

Caso grave de monkeypox associado ao diagnóstico laboratorial recente de HIV: relato de caso

Introdução: Monkeypox é uma zoonose causada pelo vírus varíola. O primeiro caso humano confirmado foi em 1970, quando o vírus foi isolado de uma criança na República Democrática do Congo. Desde o início de maio de 2022, um surto grande e inesperado tem sido documentado globalmente, com os primeiros casos inicialmente descritos no Reino Unido atingindo hoje cerca de 70 países. As causas desse aumento explosivo de pacientes não estão bem esclarecidas, mas ultrapassaram 10 mil infectados até a terceira semana de julho de 2022. As apresentações clínicas e epidemiológicas têm sido distintas dos casos endêmicos e dos pequenos surtos previamente descritos em áreas não endemicas. Objetivo: Neste relato descrevemos as características clínicas evolutivas e epidemiológicas da coinfecção do Monkeypox e do imunodeficiência humana em um paciente atendido em um serviço de referência em infecções sexualmente transmissíveis — IST/Aids de São Paulo, Brasil. Métodos: as informações dos métodos contidas neste estudo foram obtidas por meio de revisão dos prontuários, entrevistas com o paciente, prontuário fotográfico dos métodos diagnósticos, aos quais o paciente foi submetido e revisão da literatura. Resultados: Homem brasileiro, sem antecedente epidemiológico de viagem, foi diagnosticado com Monkeypox por meio de reação em cadeia da polimerase. Simultaneamente a esse diagnóstico, recebeu diagnóstico laboratorial de vírus da imunodeficiência humana, uretrite por clamídia e sífilis latente tardia. Conclusão: Para reduzir o risco de disseminação do Monkeypox, são necessárias estratégias no âmbito da saúde pública, com disseminação da informação e elaboração de projetos de prevenção com informações direcionadas e recomendações para populações vulneráveis, especialmente homens que fazem sexo com homens, com bastante prudência, buscando não favorecer o desenvolvimento de estigmas como os já vivenciados no início da epidemia de imunodeficiência humana.

Titanium/iridium dioxide as anode and zirconium as cathode in multicompartment electrolyzers with immobiline membranes

Multicompartment electrolyzers with isoelec. Immobiline membranes are used for large-scale preparative protein purifn. A series of isoelec. membranes, of defined pI values, is utilized for keeping any desired species isoelec. within each compartment of the electrolyzer. It is preferable to have electrode disks of the same surface area as the membranes for a proper performance of the instrument because electrolyte solns. of low cond. are used. The use of Pt disks would be quite expensive; therefore it is proposed using Zr as a cathode and Ti/IrO2 as an anode in the electrode compartments. This pairing of electrodes seems to give the same performance as Pt wires. Also, conventional isoelec. focusing, as well as isoelec. focusing in immobilized pH gradients, both requiring a good contact area between gel and electrodes, would benefit by using flat laminae of these metals as electrodes. [on SciFinder (R)