Natural killer cell responses during SARS-CoV-2 infection and vaccination in people living with HIV-1

Natural killer (NK) cell subsets with adaptive properties are emerging as regulators of vaccine-induced T and B cell responses and are specialized towards antibody-dependent functions contributing to SARS-CoV-2 control. Although HIV-1 infection is known to affect the NK cell pool, the additional impact of SARS-CoV-2 infection and/or vaccination on NK cell responses in people living with HIV (PLWH) has remained unexplored. Our data show that SARS-CoV-2 infection skews NK cells towards a more differentiated/adaptive CD57+FcεRIγ- phenotype in PLWH. A similar subset was induced following vaccination in SARS-CoV-2 naïve PLWH in addition to a CD56bright population with cytotoxic potential. Antibody-dependent NK cell function showed robust and durable responses to Spike up to 148 days post-infection, with responses enriched in adaptive NK cells. NK cell responses were further boosted by the first vaccine dose in SARS-CoV-2 exposed individuals and peaked after the second dose in SARS-CoV-2 naïve PLWH. The presence of adaptive NK cells associated with the magnitude of cellular and humoral responses. These data suggest that features of adaptive NK cells can be effectively engaged to complement and boost vaccine-induced adaptive immunity in potentially more vulnerable groups such as PLWH

Community Engagement newsletter, Faculty of Veterinary Science, Summer, February 2019

A fruitful experience / Byron Gopper, Kira-Lee Manne & Dr Ester Botha -- Insight to prevent a bite: Mmammudu Primary School / Marco Wasserman, Bronwen van Tonder, Frederik Loggenberg, Bethany Damonse & Elana Smit -- MaVERIC - the initiative continues / Nabeelah Rajah -- Vet Books for Africa: crossing borders one book at a time / Cormé Randlehoff -- A day that changed our lives / Johan Moolman, Kabelo Ramolotja, Kirsti Addison, Elanie van Wyk & Marné Oosthuysen -- Taking a stand against rabies / Philip van Tonder.News articles with colour photos about the various community engagement projects of the Faculty of Veterinary Science, University of Pretoria.ab201

Natural killer cell responses during SARS-CoV-2 infection and vaccination in people living with HIV-1

Abstract Natural killer (NK) cell subsets with adaptive properties are emerging as regulators of vaccine-induced T and B cell responses and are specialized towards antibody-dependent functions contributing to SARS-CoV-2 control. Although HIV-1 infection is known to affect the NK cell pool, the additional impact of SARS-CoV-2 infection and/or vaccination on NK cell responses in people living with HIV (PLWH) has remained unexplored. Our data show that SARS-CoV-2 infection skews NK cells towards a more differentiated/adaptive CD57+FcεRIγ− phenotype in PLWH. A similar subset was induced following vaccination in SARS-CoV-2 naïve PLWH in addition to a CD56bright population with cytotoxic potential. Antibody-dependent NK cell function showed robust and durable responses to Spike up to 148 days post-infection, with responses enriched in adaptive NK cells. NK cell responses were further boosted by the first vaccine dose in SARS-CoV-2 exposed individuals and peaked after the second dose in SARS-CoV-2 naïve PLWH. The presence of adaptive NK cells associated with the magnitude of cellular and humoral responses. These data suggest that features of adaptive NK cells can be effectively engaged to complement and boost vaccine-induced adaptive immunity in potentially more vulnerable groups such as PLWH

Impact on hemostatic parameters of interrupting sitting with intermittent activity

Introduction: Excessive sitting has been associated with an elevated risk of vascular conditions, particularly venous thrombosis. Interrupting sitting time with intermittent physical activity can reduce venous stasis; however, impacts on other aspects of thrombogenesis are less understood. Purpose: To examine the effects of interrupting sitting time on blood coagulation and blood volume parameters in sedentary, middle-age, overweight/obese adults (11 men and 8 women; age = 53.8 T 4.9 yr, body mass index = 31.2 T 4.1 kgImj2; mean T SD). Methods: The randomized three-period, three-treatment acute crossover trial consisted of uninterrupted sitting and sitting interrupted by 2-min bouts of either light- or moderate-intensity treadmill walking every 20 min. In each trial condition, blood samples were collected at baseline before the consumption of a standardized meal (j2 h) and postintervention (5 h). Results: Plasma fibrinogen increased from baseline with uninterrupted sitting (0.24 gILj1, 95% confidence interval = 0.13–0.34, P G 0.001). Lightintensity but not moderate-intensity activity breaks attenuated the increase by 0.17 gILj1 (95% confidence interval = 0.01–0.32, P G 0.05). There were no between-condition differences in prothrombin time, activated partial thromboplastin time, von Willebrandfactor, D-dimer, or platelet count. Uninterrupted sitting reduced plasma volume and increased hematocrit, hemoglobin, and red blood cell count; effects attenuated by both light- and moderate-intensity breaks (P G 0.05). White blood cell count increased with uninterrupted sitting and further increased with moderate-intensity breaks. Mean platelet volume increased with moderate-intensity but not lightintensity breaks or uninterrupted sitting. Conclusion: Uninterrupted sitting increased fibrinogen and reduced plasma volume, with associated increases in hemoglobin and hematocrit. Activity breaks attenuated these responses, indicative of an ameliorating influence on the procoagulant effects of uninterrupted sitting

A kilonova following a long-duration gamma-ray burst at 350 Mpc

Here, we report the discovery of a kilonova associated with the nearby (350 Mpc) minute-duration GRB 211211A. In tandem with deep optical limits that rule out the presence of an accompanying supernova to $M_I > -13$ mag at 17.7 days post-burst, the identification of a kilonova confirms that this burst's progenitor was a compact object merger. While the spectrally softer tail in GRB 211211A's gamma-ray light curve is reminiscent of previous extended emission short GRBs (EE-SGRBs), its prompt, bright spikes last $\gtrsim 12$ s, separating it from past EE-SGRBs. GRB 211211A's kilonova has a similar luminosity, duration and color to AT2017gfo, the kilonova found in association with the gravitational wave (GW)-detected binary neutron star (BNS) merger GW170817. We find that the merger ejected $\approx 0.04 M_{\odot}$ of r-process-rich material, and is consistent with the merger of two neutron stars (NSs) with masses close to the canonical $1.4 M_{\odot}$. This discovery implies that GRBs with long, complex light curves can be spawned from compact object merger events and that a population of kilonovae following GRBs with durations $\gg 2$ s should be accounted for in calculations of the NS merger r-process contribution and rate. At 350 Mpc, the current network of GW interferometers at design sensitivity would have detected the merger precipitating GRB 211211A, had it been operating at the time of the event. Further searches for GW signals coincident with long GRBs are therefore a promising route for future multi-messenger astronomy.Comment: Submitted. 69 pages, 11 figures, 3 table