Improving dental epithelial junction on dental implants with bioengineered peptides

Introduction: The functionalization of titanium (Ti) and titanium alloys (Ti6Al4V) implant surfaces via material-specific peptides influence host/biomaterial interaction. The impact of using peptides as molecular linkers between cells and implant material to improve keratinocyte adhesion is reported.Results: The metal binding peptides (MBP-1, MBP-2) SVSVGMKPSPRP and WDPPTLKRPVSP were selected via phage display and combined with laminin-5 or E-cadherin epithelial cell specific peptides (CSP-1, CSP-2) to engineer four metal-cell specific peptides (MCSPs). Single-cell force spectroscopy and cell adhesion experiments were performed to select the most promising candidate. In vivo tests using the dental implant for rats showed that the selected bi functional peptide not only enabled stable cell adhesion on the trans-gingival part of the dental implant but also arrested the unwanted apical migration of epithelial cells.Conclusion: The results demonstrated the outstanding performance of the bioengineered peptide in improving epithelial adhesion to Ti based implants and pointed towards promising new opportunities for applications in clinical practice

Improving dental epithelial junction on dental implants with bioengineered peptides

Introduction: The functionalization of titanium (Ti) and titanium alloys (Ti6Al4V) implant surfaces via material-specific peptides influence host/biomaterial interaction. The impact of using peptides as molecular linkers between cells and implant material to improve keratinocyte adhesion is reported.Results: The metal binding peptides (MBP-1, MBP-2) SVSVGMKPSPRP and WDPPTLKRPVSP were selected via phage display and combined with laminin-5 or E-cadherin epithelial cell specific peptides (CSP-1, CSP-2) to engineer four metal-cell specific peptides (MCSPs). Single-cell force spectroscopy and cell adhesion experiments were performed to select the most promising candidate. In vivo tests using the dental implant for rats showed that the selected bi functional peptide not only enabled stable cell adhesion on the trans-gingival part of the dental implant but also arrested the unwanted apical migration of epithelial cells.Conclusion: The results demonstrated the outstanding performance of the bioengineered peptide in improving epithelial adhesion to Ti based implants and pointed towards promising new opportunities for applications in clinical practice

Biofuctionalization of semiconductors via peptides extracted by phage display technology.

Thes

Peptide

Implantology biomatrials</p

lumbar lordosis

   Evaluation of the accuracy of new modalities in the assessment and classification of lumbar lordosis: A comparison to Cobb’s angle measurement</p

Biofunctionalization of semiconductors via peptides extracted by phage display technology (Biosensing applications on related devices)

La réalisation de dispositifs hybrides (organique /inorganique) est indispensable dans le domaine biomédical, et des nanotechnologies, pour le développement de nouveaux matériaux et la réalisation de biocapteurs. Pour les applications de biodétection, le problème principal est de rendre les matériaux inorganiques compatibles, stables et non toxiques. Dans le passé, de nombreuses méthodes ont été étudiées; nous proposons une méthode alternative prometteuse qui est la fonctionnalisation avec des peptides qui reconnaissent spécifiquement certains matériaux. Les peptides d'adhésion pour treize semi-conducteurs, le GaAs (100), GaAs (111), GaN (0001), InAs (100), InP (001), InP (111), GaSb (100), ZnTe (100), ZnSe (100), CdSe (100), Si (100) dopé (n), et deux types de Si (100) dopé (p) ont été élaborés par la technique phage display . La réelle affinité des peptides obtenus a été étudiée avec des techniques microscopiques et spectroscopiques. Ensuite, le peptide spécifique du silicium fortement dopé (p) a été utilisé pour la fonctionnalisation du silicium poreux. L'adsorption et la détection d'une biomolécule ont été suivies principalement par réflectivité spéculaire et les résultats ont été comparés avec la méthode de silanisation. Les résultats démontrent une nette amélioration dans le seuil de détection moléculaire quand les biocapteurs sont fonctionnalisés avec les peptides. La fonctionnalisation et le couplage d'une biomolécule à un cristal photonique ont été suivis en optique non-linéaire. L'amplification de la génération de seconde harmonique a été révélée suite à la liaison peptide biotinylé-streptavidine, conduisant à une haute sensibilité de détection moléculaire.The realization of hybrid devices (organic / inorganic) is indispensable in the biomedical field for the progress of nanotechnology, the development of new materials and fabrication of biosensors. For biosensing applications, the challenge is to render the used inorganic materials compatible, stable, and non-toxic by biofonctionnalisation. Although in the past a number of surface modification methods have been used, we propose an original, alternative way of functionalization with peptides that specifically recognize semiconductor surfaces. The adhesion peptides for thirteen semiconductors, the GaAs (100), GaAs (111), GaN (0001), InAs (100), InP (001), InP (111), GaSb (100), ZnTe (100), ZnSe (100), CdSe (100), n-doped Si (100), and two types of p-doped Si (100) were elaborated via the "phage display" technology. Real affinity of the obtained specific peptides was extensively studied by microscopic and spectroscopic techniques. Then, the specific peptide expressed for the highly (p) doped silicon was used for the functionalization of porous silicon; adsorption and detection of biomolecules were monitored primarily by the specular reflectivity. When comparing with the traditional silanization method, functionalizing porous silicon, via the peptide- route, reveals high quality surfaces where molecular detection is much enhanced. Functionalization and coupling of a biomolecule to a photonic crystal were monitored by non-linear optics. Enhanced second-harmonic generation was observed when streptavidin bound to biotinylated peptide, leading to a high sensitivity molecular detection.MONTPELLIER-BU Sciences (341722106) / SudocSudocFranceF

Biosensing Using a Simple Resistor: The Effect of Functionalization on Sensing Devices

Biosensors can play an important role in early disease detection, a reason why they are gaining more attention in the world of biomedicine. Functionalization of the material used in the detector is of a great importance since it maintains the molecule’s structure of interest with minimal changes. We report on sensing BSA molecules, solutions, and concentrations using a functionalized commercial resistor in a simple electric circuit. Our results demonstrate the outstanding utility of functionalization in biosensing devices; while sensing is not possible with a naked resistor, a BSA covered resistor can detect a very low solution concentration around 0.1 fM. A smaller molecule like tryptophan was also used in order to functionalize the resistor. After proving that tryptophan is more effective in covering the resistor before sensing, BSA molecules in other solvent conditions were detected, and a threshold of 1 μM was obtained. This can prove that sensing depends on the choice of the functionalizations of the material used for sensing and on the conformation of the molecule of interest in its solutions. This method of detection may be of great interest in triggering and sensing biological molecules using simple-based devices

Peptide Route Functionalization of ZnSe Crystals Preserves Activity and Structure of Proteins while Adsorption

International audienc

Significance of Hydroxyl Groups on the Optical Properties of ZnO Nanoparticles Combined with CNT and PEDOT:PSS

We report on the synthesis of ZnO nanoparticles and their hybrids consisting of carbon nanotubes (CNT) and polystyrene sulfonate (PEDOT:PSS). A non-aqueous sol&ndash;gel route along with hydrated and anhydrous acetate precursors were selected for their syntheses. Transmission electron microscopy (TEM) studies revealed their spherical shape with an average size of 5 nm. TEM also confirmed the successful synthesis of ZnO-CNT and ZnO-PEDOT:PSS hybrid nanocomposites. In fact, the choice of precursors has a direct influence on the chemical and optical properties of the ZnO-based nanomaterials. The ZnO nanoparticles prepared with anhydrous acetate precursor contained a high amount of oxygen vacancies, which tend to degrade the polymer macromolecule, as confirmed from X-ray photoelectron spectroscopy and Raman spectroscopy. Furthermore, a relative increase in hydroxyl functional groups in the ZnO-CNT samples was observed. These functional groups were instrumental in the successful decoration of CNT and in producing the defect-related photoluminescence emission in ZnO-CNT