Trypanosoma rangeli is phylogenetically closer to Old World trypanosomes than to Trypanosoma cruzi.

This is the author accepted manuscript. The final version is available from Elsevier via the DOI in this record.Trypanosoma rangeli and Trypanosoma cruzi are generalist trypanosomes sharing a wide range of mammalian hosts; they are transmitted by triatomine bugs, and are the only trypanosomes infecting humans in the Neotropics. Their origins, phylogenetic relationships, and emergence as human parasites have long been subjects of interest. In the present study, taxon-rich analyses (20 trypanosome species from bats and terrestrial mammals) using ssrRNA, glycosomal glyceraldehyde-3-phosphate dehydrogenase (gGAPDH), heat shock protein-70 (HSP70) and Spliced Leader RNA sequences, and multilocus phylogenetic analyses using 11 single copy genes from 15 selected trypanosomes, provide increased resolution of relationships between species and clades, strongly supporting two main sister lineages: lineage Schizotrypanum, comprising T. cruzi and bat-restricted trypanosomes, and Tra[Tve-Tco] formed by T. rangeli, Trypanosoma vespertilionis and Trypanosoma conorhini clades. Tve comprises European T. vespertilionis and African T. vespertilionis-like of bats and bat cimicids characterised in the present study and Trypanosoma sp. Hoch reported in monkeys and herein detected in bats. Tco included the triatomine-transmitted tropicopolitan T. conorhini from rats and the African NanDoum1 trypanosome of civet (carnivore). Consistent with their very close relationships, Tra[Tve-Tco] species shared highly similar Spliced Leader RNA structures that were highly divergent from those of Schizotrypanum. In a plausible evolutionary scenario, a bat trypanosome transmitted by cimicids gave origin to the deeply rooted Tra[Tve-Tco] and Schizotrypanum lineages, and bat trypanosomes of diverse genetic backgrounds jumped to new hosts. A long and independent evolutionary history of T. rangeli more related to Old World trypanosomes from bats, rats, monkeys and civets than to Schizotrypanum spp., and the adaptation of these distantly related trypanosomes to different niches of shared mammals and vectors, is consistent with the marked differences in transmission routes, life-cycles and host-parasite interactions, resulting in T. cruzi (but not T. rangeli) being pathogenic to humans.This study was supported by grants awarded to MMGT and EPC from CNPq (National Council for Scientific and Technological Development) PROAFRICA, PROSUL and UNIVERSAL programs, CAPES (Coordination for the Improvement of Higher Education Personnel) PNIPB, PNPD and PROTAX programs, and FAPESP (São Paulo Research Foundation; process 2016/07487-0). Genome sequencing was supported by the Assembling the Tree of Life (ATOL) Project of the National Science Foundation, USA (NSF DEB-0830056), and TCC-USP (Trypanosomatid Culture Collection of the University of São Paulo) projects. OEA received PhD fellowships from CNPq (PROTAX) and COLCIENCIAS (Administrative Department of Science, Technology and Innovation, Colombia); PAO is a postdoctoral fellow of CAPES (PNPD); LL and AGCM are supported by a postdoctoral fellowship from CAPES (PROTAX)

El acceso intraoseo en reanimación pediátrica. 1999-2001. Intra-osseous approach in pediatric resuscitation. 2001-2002

El establecimiento de un acceso en la circulación es un componente crítico en la resucitación. La infusión intraósea fue descrita por primera vez en 1922 y utilizada para la administración de drogas en 1940, permitiendo un rápido acceso intravascular en pacientes críticamente enfermos, con el objetivo de demostrar sus beneficios en la reanimación infantil en situaciones emergentes. Se realizó una investigación longitudinal, prospectiva y descriptiva en el servicio de cuidados intensivos pediátricos del Hospital General Docente Comandante Pinares, San Cristóbal. Pinar del Río. Cuba. En la etapa comprendida entre enero-1999 a diciembre-2001. Se estudiaron 11 pacientes menores de 5 años que necesitaron medidas de reanimación, realizándose una encuesta que contaba con las siguientes variables: edad, diagnóstico al ingreso, tiempo de obtención de la vía y mantenimiento, drogas, fluidos administrados y complicaciones, se le aplicó el método de estadística descriptivo, distribución de frecuencia y test de proporción. Se concluyó que el 28,2 % (11) de la muestra requirió canalización intraósea, los lactantes (menores de 6 meses) con deshidratación severa necesitaron esta vía en el 54,5% (6), se logró obtener en un tiempo menor de un minuto en 81,8% (7) de los pacientes y se mantuvo hasta 4 horas en el 90,9% de los casos estudiados, las drogas y fluidos mayormente administrados fueron las catecolaminas y la solución salina fisiológica en el 63,6% y el 100% respectivamente, es una técnica con muy raras complicaciones reportándose solo un 9,1%. DeCS: INFUSIÓN INTRAÓSEA, SITUACIONES EMERGENTES, REANIMACIÓN, CATECOLAMINAS, ACCESO INTRAVASCULAR. ABSTRACTThe establishment of an access in Circulation is a very important component in resuscitation. The intraosseous infusion was described for the first time in 1922 and use to administer medications in 1940 allowing a fast intravascular access in critically ill patients. A longitudinal, prospective and descriptive research was carried out at Intensive Care Pediatric Unit belonging to Comandante Pinares General Hospital, San Cristobal, Pinar del Rio aimed at showing its benefits in child resuscitation mainly in emergent situations. During January 1999 to December 2001 eleven patients under 5 years old needing resuscitation measures were studied. A survey taking into account age, diagnosis at admission, time obtained for route and maintenance, drugs, administration of fluids and complications was conducted. Descriptive method, frequency distribution and proportion test were statistically used. Concluding that 28.2 % (11) of the sample required intraosseous infusions, infants (under 6 months) suffering from severe dehydration needed this via (54.5 %) (6), it was obtained (less than a minute) the 81.8 % (7) of the patients and the maintenance was 4 hours in the 90.9 % of the cases studied, drugs and fluids having a greater administration were catecholamines and hypertonic saline solution in 63.6 % and in 100 % of the cases respectively, this technique provoked scarcely complications, only 9.1 % could be reported. DeCS: INTRAOSSEOUS INFUSION, CRITICAL CARE, RESUSCITATION, CATECHOLAMINES, INTRAVASCULAR ACCESS

El acceso intraoseo en reanimación pediátrica. 1999-2001. Intra-osseous approach in pediatric resuscitation. 2001-2002

El establecimiento de un acceso en la circulación es un componente crítico en la resucitación. La infusión intraósea fue descrita por primera vez en 1922 y utilizada para la administración de drogas en 1940, permitiendo un rápido acceso intravascular en pacientes críticamente enfermos, con el objetivo de demostrar sus beneficios en la reanimación infantil en situaciones emergentes. Se realizó una investigación longitudinal, prospectiva y descriptiva en el servicio de cuidados intensivos pediátricos del Hospital General Docente Comandante Pinares, San Cristóbal. Pinar del Río. Cuba. En la etapa comprendida entre enero-1999 a diciembre-2001. Se estudiaron 11 pacientes menores de 5 años que necesitaron medidas de reanimación, realizándose una encuesta que contaba con las siguientes variables: edad, diagnóstico al ingreso, tiempo de obtención de la vía y mantenimiento, drogas, fluidos administrados y complicaciones, se le aplicó el método de estadística descriptivo, distribución de frecuencia y test de proporción. Se concluyó que el 28,2 % (11) de la muestra requirió canalización intraósea, los lactantes (menores de 6 meses) con deshidratación severa necesitaron esta vía en el 54,5% (6), se logró obtener en un tiempo menor de un minuto en 81,8% (7) de los pacientes y se mantuvo hasta 4 horas en el 90,9% de los casos estudiados, las drogas y fluidos mayormente administrados fueron las catecolaminas y la solución salina fisiológica en el 63,6% y el 100% respectivamente, es una técnica con muy raras complicaciones reportándose solo un 9,1%. DeCS: INFUSIÓN INTRAÓSEA, SITUACIONES EMERGENTES, REANIMACIÓN, CATECOLAMINAS, ACCESO INTRAVASCULAR. ABSTRACTThe establishment of an access in Circulation is a very important component in resuscitation. The intraosseous infusion was described for the first time in 1922 and use to administer medications in 1940 allowing a fast intravascular access in critically ill patients. A longitudinal, prospective and descriptive research was carried out at Intensive Care Pediatric Unit belonging to Comandante Pinares General Hospital, San Cristobal, Pinar del Rio aimed at showing its benefits in child resuscitation mainly in emergent situations. During January 1999 to December 2001 eleven patients under 5 years old needing resuscitation measures were studied. A survey taking into account age, diagnosis at admission, time obtained for route and maintenance, drugs, administration of fluids and complications was conducted. Descriptive method, frequency distribution and proportion test were statistically used. Concluding that 28.2 % (11) of the sample required intraosseous infusions, infants (under 6 months) suffering from severe dehydration needed this via (54.5 %) (6), it was obtained (less than a minute) the 81.8 % (7) of the patients and the maintenance was 4 hours in the 90.9 % of the cases studied, drugs and fluids having a greater administration were catecholamines and hypertonic saline solution in 63.6 % and in 100 % of the cases respectively, this technique provoked scarcely complications, only 9.1 % could be reported. DeCS: INTRAOSSEOUS INFUSION, CRITICAL CARE, RESUSCITATION, CATECHOLAMINES, INTRAVASCULAR ACCESS

A mid-infrared view of the inner parsecs of the Seyfert galaxy Mrk 1066 using CanariCam/GTC

We present mid-infrared (MIR) imaging and spectroscopic data of the Seyfert 2 galaxy Mrk 1066 obtained with CanariCam (CC) on the 10.4-m Gran Telescopio CANARIAS (GTC). The galaxy was observed in imaging mode with an angular resolution of 0.24 arcsec (54 pc) in the Si-2 filter (8.7 μm). The image reveals a series of star-forming knots within the central ∼400 pc, after subtracting the dominant active galactic nucleus (AGN) component. We also subtracted this AGN unresolved component from the 8–13 μm spectra of the knots and the nucleus, and measured equivalent widths (EWs) of the 11.3 μm polycyclic aromatic hydrocarbon (PAH) feature which are typical of pure starburst galaxies. This EW is larger in the nucleus than in the knots, confirming that, at least in the case of Mrk 1066, the AGN dilutes, rather than destroys, the molecules responsible for the 11.3 μm PAH emission. By comparing the nuclear GTC/CC spectrum with the Spitzer/Infrared Spectrograph (IRS) spectrum of the galaxy, we find that the AGN component that dominates the continuum emission at λ < 15 μm on scales of ∼60 pc (90–100 per cent) decreases to 35–50 per cent when the emission of the central ∼830 pc is considered. On the other hand, the AGN contribution dominates the 15–25 μm emission (75 per cent) on the scales probed by Spitzer/IRS. We reproduced the nuclear infrared emission of the galaxy with clumpy torus models, and derived a torus gas mass of 2 × 10^5  M_⊙, contained in a clumpy structure of ∼2 pc radius and with a column density compatible with Mrk 1066 being a Compton-thick candidate, in agreement with X-ray observations. We find a good match between the MIR morphology of Mrk 1066 and the extended Paβ, Brγ and [O iii] λ5007 emission. This coincidence implies that the 8.7 μm emission is probing star formation, dust in the narrow-line region and the oval structure previously detected in the near-infrared. On the other hand, the Chandra soft X-ray morphology does not match any of the previous, contrary to what it is generally assumed for Seyfert galaxies. A thermal origin for the soft X-ray emission, rather than AGN photoionization, is suggested by the different data analysed here

Additional value of screening for minor genes and copy number variants in hypertrophic cardiomyopathy

Introduction: Hypertrophic cardiomyopathy (HCM) is the most prevalent inherited heart disease. Next-generation sequencing (NGS) is the preferred genetic test, but the diagnostic value of screening for minor and candidate genes, and the role of copy number variants (CNVs) deserves further evaluation. Methods: Three hundred and eighty-seven consecutive unrelated patients with HCM were screened for genetic variants in the 5 most frequent genes (MYBPC3, MYH7, TNNT2, TNNI3 and TPM1) using Sanger sequencing (N = 84) or NGS (N = 303). In the NGS cohort we analyzed 20 additional minor or candidate genes, and applied a proprietary bioinformatics algorithm for detecting CNVs. Additionally, the rate and classification of TTN variants in HCM were compared with 427 patients without structural heart disease. Results: The percentage of patients with pathogenic/likely pathogenic (P/LP) variants in the main genes was 33.3%, without significant differences between the Sanger sequencing and NGS cohorts. The screening for 20 additional genes revealed LP variants in ACTC1, MYL2, MYL3, TNNC1, GLA and PRKAG2 in 12 patients. This approach resulted in more inconclusive tests (36.0% vs. 9.6%, p<0.001), mostly due to variants of unknown significance (VUS) in TTN. The detection rate of rare variants in TTN was not significantly different to that found in the group of patients without structural heart disease. In the NGS cohort, 4 patients (1.3%) had pathogenic CNVs: 2 deletions in MYBPC3 and 2 deletions involving the complete coding region of PLN. Conclusions: A small percentage of HCM cases without point mutations in the 5 main genes are explained by P/LP variants in minor or candidate genes and CNVs. Screening for variants in TTN in HCM patients drastically increases the number of inconclusive tests, and shows a rate of VUS that is similar to patients without structural heart disease, suggesting that this gene should not be analyzed for clinical purposes in HCM

Flower Development as an Interplay between Dynamical Physical Fields and Genetic Networks

In this paper we propose a model to describe the mechanisms by which undifferentiated cells attain gene configurations underlying cell fate determination during morphogenesis. Despite the complicated mechanisms that surely intervene in this process, it is clear that the fundamental fact is that cells obtain spatial and temporal information that bias their destiny. Our main hypothesis assumes that there is at least one macroscopic field that breaks the symmetry of space at a given time. This field provides the information required for the process of cell differentiation to occur by being dynamically coupled to a signal transduction mechanism that, in turn, acts directly upon the gene regulatory network (GRN) underlying cell-fate decisions within cells. We illustrate and test our proposal with a GRN model grounded on experimental data for cell fate specification during organ formation in early Arabidopsis thaliana flower development. We show that our model is able to recover the multigene configurations characteristic of sepal, petal, stamen and carpel primordial cells arranged in concentric rings, in a similar pattern to that observed during actual floral organ determination. Such pattern is robust to alterations of the model parameters and simulated failures predict altered spatio-temporal patterns that mimic those described for several mutants. Furthermore, simulated alterations in the physical fields predict a pattern equivalent to that found in Lacandonia schismatica, the only flowering species with central stamens surrounded by carpels

Constraints on the χ_(c1) versus χ_(c2) polarizations in proton-proton collisions at √s = 8 TeV

The polarizations of promptly produced χ_(c1) and χ_(c2) mesons are studied using data collected by the CMS experiment at the LHC, in proton-proton collisions at √s=8  TeV. The χ_c states are reconstructed via their radiative decays χ_c → J/ψγ, with the photons being measured through conversions to e⁺e⁻, which allows the two states to be well resolved. The polarizations are measured in the helicity frame, through the analysis of the χ_(c2) to χ_(c1) yield ratio as a function of the polar or azimuthal angle of the positive muon emitted in the J/ψ → μ⁺μ⁻ decay, in three bins of J/ψ transverse momentum. While no differences are seen between the two states in terms of azimuthal decay angle distributions, they are observed to have significantly different polar anisotropies. The measurement favors a scenario where at least one of the two states is strongly polarized along the helicity quantization axis, in agreement with nonrelativistic quantum chromodynamics predictions. This is the first measurement of significantly polarized quarkonia produced at high transverse momentum