Wpływ aplikacji różnych substancji i metod szczepienia na powodzenie szczepienia i wzrost morwy czarnej (Morus nigra L.)

This research was conducted to determine the effects of three grafting methods (chip budding, side and splice grafting) and topical applications on graft scion of IBA (0 and 4000 ppm) and three plant growth promoting rhizobacteria (PGPR) strains (Bacillus subtilus-OSU142, Bacillus megatorium-M3 and Burkholderia gladia-BA7) on graft success and plant growth in black mulberry sapling production. The experiment was carried out in a randomized complete block design with three replications in Bolu, Turkey in 2014 and 2015. The black mulberry (Morus nigra L.) were used for grafting scions. Twoyear old white mulberry (Morus alba L.) seedling were used for rootstocks. The results showed that in general all of the bacterial strains and IBA had significant effects on all parameters tested in graft scion compared with the control. Application of 4000 ppm IBA and Bacillus megatorium-M3 increased the success graft take rate (74.44 and 72.22%, respectively), graft sprouting rate (61.11 and 60.00%, respectively), graft shoot diameter (6.21 and 5.70 mm, respectively) and graft shoot length (35.50 and 35.31 cm, respectively). Grafting methods had significant effect for all parameters and grafting methods increased the graft success. The best graft take rates (75.33 and 70.67%, respectively), graft sprouting rates (62.67 and 57.33% respectively), graft shoot diameter (5.56 and 5.88 mm, respectively) and graft shoot length (34.14 and 37.86 cm, respectively) were obtained from splice grafting and chip budding methods. In conclusion, the present study showed that 4000 ppm IBA and PGPR strains (especially M3 and OSU142) increased the graft success of black mulberry graft. The PGPR application may be of benefit for grafting in mulberry cultivars, particularly for organic farming. Besides, splice grafting and chip budding were found to be successful to black mulberry plant production.Badanie przeprowadzono w celu ustalenia wpływu trzech metod szczepienia (okulizacja na przystawkę, szczepienie boczne i w klin) szczytowej aplikacji IBA (0 i 4000 ppm) i trzech szczepów ryzobakterii (PGPR) wspierających wzrost roślin (Bacillus subtilus-OSU142, Bacillus megatorium-M3 i Burkholderia gladia-BA7) na powodzenie szczepienia i wzrost roślin w produkcji sadzonek morwy czarnej. Doświadczenie przeprowadzono w miejscowości Bolu w Turcji w latach 2014 i 2015 w układzie bloków losowych w trzech powtórzeniach. Czarna morwa (Morus nigra L.) była użyta do cięcia. Dwuletnie sadzonki białej morwy (Morus alba L.) użyto jako podkładki. Wyniki wykazały, że ogólnie wszystkie szczepy bakterii oraz IBA miały istotny wpływ na wszystkie badane parametry cięcia w porównaniu z kontrolą. Użycie 4000 ppm IBA oraz Bacillus megatorium-M3 zwiększało wskaźnik powodzenia szczepień (odpowiednio 74,44 i 72,22%), wskaźnik kiełkowania szczepów (odpowiednio 61,11 i 60,00%), średnicę pędów szczepów (odpowiednio 6,21 i 5,70 mm) oraz długość pędów szczepów (odpowiednio 35.50 i 35.31 cm). Metody szczepień miały istotny wpływ na wszystkie parametry i zwiększały powodzenie szczepienia. Najlepsze wskaźniki powodzenia szczepień osiągnięto w przypadku metod w klin i okulizacji. Podsumowując, badanie wykazało, że 4000 ppm IBA i szczepy PGPR (zwłaszcza M3 i OSU142) zwiększały powodzenie szczepienia czarnej morwy. Zastosowanie PGPR może być korzystne w szczepieniu odmian morwy, zwłaszcza w uprawach organicznych. Poza tym stwierdzono, że szczepienie w klin i okulizacja mogą być z powodzeniem stosowane w szczepieniu morwy czarnej

Effects of some growing media on the growth of strawberry cvs. 'Camarosa' and 'Fern'

This study evaluated the development of above and under ground parts of strawberry plants cvs. 'Fern' and 'Camarosa1 in a glasshouse using perlite, forest soil, finpeat, peat, finpeat+perlite (1:1) and peat+perlite (1:1) as substrates. The effects of different growing media on development of above and under ground parts of plant were statistically significant. The highest number of primary roots per plant, length of the most developed roots and number of crowns per runner were obtained from perlite, finpeat+perlite and peat medium, respectively, in both cultivars. The runner length, number of runners per plant, leaf area, fresh root weight and dry root weight varied between cultivars according to growth medium. In general peat, finpeat or finpeat+perlite gave the best results in terms of above and under ground parts of plants in both cultivars. Some important properties such as water retention capacity, cation exchangeable capacity, pH, porosity etc. of growing media using in this study were also determined

Biocontrol of Botrytis Cinerea on strawberry fruit by plant growth promoting bacteria

In the present study, a total of 186 bacterial strains isolated from various soil sources and plant species from Eastern Anatolia region in Turkey were evaluated for their ability to suppress gray mold (Botrytis cinerea Pers. ex Fr.) occurred on strawberry cv. Fern. Among 186 bacterial strains, 36 were found effective to inhibit of development B. cinerea under in vitro conditions, and thirteen of them which have greater inhibition zone were selected as biocontrol agent. These antagonistic strains were identified as Bacillus lentimorbus, B. megaterium, B. pumilis, B. subtilis, Enterobacter intermedius, Kurthia sibirica, Paenibacillus polymyxa and Pantoea agglomerans. The inhibition zones among bacteria against B. cinerea were found between 0.50 (Bacillus C6, Brevibacterium MFD-47 and Pantoea MFD-232) and 3.75 cm (Enterobacter MFD-81) in vitro. The strawberry fruits were inoculated with B. cinerea in laboratory trials and Bacillus MFDÜ-2 (14.41 mm) was found more effective to prevent mycelial development on strawberry fruits in comparison to the control (19.20 mm). In terms of conidia germination on strawberry fruits, the lowest disease incidence was observed in MFD-45 treatment (20.8%), while disease incidence rate for the control was 79.2%. It was shown that antagonistic bacterial strains inhibited B. cinerea and that they have a potential use in sustainable strawberry production

Orchard performance of apple worsens as weed competition increases: a long-term field study under Mediterranean conditions

Crop-weed competition (CWC) for water and nutrients can negatively affect orchard performance, especially in high-density apple orchards. With this study, we compared the effects of three CWC levels: Weak, Moderate, and Strong for the orchard performance of apple in the Mediterranean area. The study was conducted at a ‘Golden Delicious’/M.9 orchard in a high-density formation (3.5 × 1 m spacing). Cumulative yield was greatest in Weak-CWC, and it decreased as CWC increased. Overall, Weak-CWC performed remarkable results for successful apple production under Mediterranean conditions

Plant Growth-Promoting Rhizobacteria (Pgpr) Increase Yield, Growth And Nutrition Of Strawberry Under High-Calcareous Soil Conditions

Plant growth promoting effects of Alcaligenes 637Ca, Staphylococcus MFDCa-1, MFDCa-2, Agrobacterium A18, Pantoea FF1 and Bacillus M3 were tested on strawberry cv. 'Aromas' based on yield, number, and weight of fruit, leaf area, vitamin C, total soluble solids (TSS), acidity and ionic composition of leaves under calcareous soil conditions. The results demonstrated that all of bacterial treatments significantly affected all parameters tested. The best result was obtained from 637Ca treatment, which significantly increased fruit yield, number and weight about 47.5, 34.7, and 9.4%, respectively, compared to control. Except for magnesium (Mg) and zinc (Zn) in the leaf, the concentrations of all plant tissue nutrients [nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), iron (Fe), copper (Cu), manganese (Mn), boron (B)] were significantly increased by bacterial treatments tested. The data in the present study showed that all bacterial treatments including Alcaligenes 637Ca, Staphylococcus MFDCa-1, MFDCa-2, Agrobacterium A18, Pantoea FF1, and Bacillus M3 to strawberry plants can ameliorative the deleterious effect of high lime on fruit yield, growth and nutrition. These results suggested that plant growth-promoting Rhizobacteria (PGPR) treatments could be offer an economic and simple means to increased plant resistance for high calcareous soil conditions. 2014 © Taylor & Francis Group, LLC

Genetic characterization of pomegranate (Punica granatum L.) genotypes by AFLP markers

PubMedID: 22446597The Coruh Valley, located in Northeastern Turkey, is one of the most important centers of diversity in pomegranate in Turkey. In this study, we attempted to characterize 19 promising pomegranate genotypes originating from the Coruh Valley in using fluorescent dye AFLP markers and capillary electrophoresis. Four AFLP primer combinations were used, generating a total of 297 fragments, 213 of which were polymorphic (73.0%). Resolving powers of the AFLP primers ranged from 0.700 to 1.018, with a total of 3.440, while polymorphism information contents ranged from 0.707 to 0.837 with an average of 0.764. UPGMA clustering of the genotypes showed two major groups. Most of the fruit characteristics of the genotypes within the same group were variable. Therefore, the results showed that molecular characterization is necessary to get reliable relationships among pomegranate genotypes and AFLP markers can be used effectively in pomegranate

Simple sequence repeat-based assessment of genetic relationships among Prunus rootstocks

Ten SSR loci, previously developed for Prunus, were analyzed to examine genetic relationships among 23 rootstock candidates for sweet and sour cherries, of the species P. avium, P. cerasus, P. mahaleb, and P. angustifolia. Five genotypes of P. laurocerasus, not used as rootstock, were included in the molecular analysis. The number of alleles per locus ranged from 8 to 12, with a mean of 9, while the number of microsatellite genotypes varied from 8 to 17, indicating that the SSRs were highly informative. The degree of heterozygosity (0.61) was high. Clustering analysis resulted in two main clusters. The first cluster was divided into two subclusters; the first subcluster consisted of P. avium and P. cerasus, and the second subcluster consisted of P. laurocerasus. The second cluster was divided into two subclusters. The first subcluster consisted of P. mahaleb genotypes and the second consisted of P. angustifolia genotypes. The reference rootstocks also clustered with their associated botanical species. Unweighted pair-group method with arithmetic mean analysis demonstrated that P. laurocerasus genotypes had less genetic variation and that P. avium genotypes were more closely related to P. cerasus. The SSR-based phylogeny was generally consistent with Prunus taxonomy information, suggesting the applicability of SSR analysis for genotyping and phylogenetic studies in the genus Prunus

The Effect of Weed Competition on Apple Fruit Quality

Orchard performance is influenced by weed competition. In this study, the effects of weed competition on nutrient contents, chemical and physical fruit quality properties were sought. The study was carried out in a high-density apple orchard (‘Golden Delicious’/M.9) over two consecutive growing seasons. The effect of weed competition was studied at three different levels: weak, moderate and strong. Fruit firmness, soluble solids content, macronutrients (such as nitrogen, potassium and calcium) and potassium+magnesium/calcium ratio in fruit were significantly affected by weed competition. Strong weed competition negatively affected soluble solids content and potassium+magnesium/calcium ratio. In both trial years, soluble solids content was significantly higher in weak weed competition. In the first year of the study, soluble solids content ranged between 13.77±0.06% (strong weed competition) and 15.20±0.10% (weak weed competition). In the following year, soluble solids content values were determined as 13.13±0.23% in strong weed competition and 13.83±0.21% in weak weed competition. Weak weed competition showed superiority for fruit weight and potassium+magnesium/calcium ratio. As a whole, this study indicates that insufficient weed control in tree rows might be a limiting factor for fruit quality in high-density apple orchards