Upaya Pencegahan Periodontitis Agresif dengan Obat Kumur Minosiklin.

Periodontitis agresif adalah penyakit pada jaringan penyangga gigi yang ditandai dengan kerusakan yang cepat pada ligamen periodontal dan tulang alveol pada penderita usia muda. Periodontitis agresif pada umumnya terjadi pada usia di bawah 30 tahun dan yang sering menjadi sasaran penyakit tersebut adalah gigi geraham dan gigi seri. Pada penyakit periodontitis agresif proses kehilangan perlekatan jaringan dan resesi gingiva tiga kali lebih cepat dibanding periodontitis kronis, tulang penyangga gigi dapat mengalami resorbsi menjadi 60% hanya dalam waktu sembilan bulan (Velden dkk, 2006). Gejala awalnya ditandai gigi goyang, yang diikuti perubahan posisi gigi misalnya, gigi seri tampak lebih maju, antar gigi menjadi renggang, mahkota gigi tampak memanjang, bahkan gigi dapat terlepas dari tempat tertanamnya tanpa sebab

Susu kedelai meningkatkan jumlah osteoblast tulang alveolar tikus wistar yang diinduksi oleh A actinomycetemcomitans.

Background: Periodontitis can be caused by Aggregatebacter actinomycetemcomitans bacteria. This bacterium induces inflammation in periodontal tissues and can lead into periodontal tissues breakdown and destruction of the alveolar bone and decrease the number of osteoblast cells. Soy milk containing the amino acid glycine and Amino Acids Arginine Insulin is able to maintain balance. In addition, the protein in soy milk is more easily accepted kidneys compared with animal protein. Soy milk stimulate the osteoblastic activity of estrogen receptors, and increases production of growth hormone – (insuline Like Growth Factor 1 (IGF-1). Purpose: The aim of this study is find out the soy milk effect on the elevation of osteoblast in alveolar bone of wistar rats induced A. actinomycetemcomitans. Methods: In this study, there are 27 male wistar rats were divided into three groups: control, AA, and AA with soymilk. In AA group, Aggregatibacter actinomycetemcomitans bacteria was given in first, third, fifth day of one month experiment. In AA and soymilk group was given both Aggregatibacter actinomycetemcomitans bactery and soymilk. At the end of one month, the rats were sacrificed, the alveolar bone tissue were dissected by microtome and it was analyzed for osteoblast level by microscope using hematoxylin eosin staining. Result: AA group resulted in significantly reductions in the alveolar bone tissue level of osteoblast than the control (p<0.05). Soy milk group show significant elevation of osteoblast compared to AA group (p<0.05). And the result of the present study indicates that soy milk show significant elevation in alveolar bone level of osteoblast in Aggregatibacter actinomycetemcomitan-induced experimental periodontitis. Conclusion: Soy milk administration for a month showed elevation of osteoblast number in alveolar bone of wistar rats induced A. actinomycetemcomitans

Nigella sativa oral rinseas an anti oxidant effect reduced bleeding on probing and pocket depth

Nigella sativa extract have been shown to suppress the growth of supragingival and subgingival bacteria. Nigella sativa extract were reported as an anti inflammation. The beneficial effects of nigella sative extract were shown to be related to a reduction of the inhibition of inducible nitric oxide synthase and interleukin (IL)-1{3 expression. The rationale of the study is based on our previous studies demonstrating the beneficial antioxidant effect of nigella sativa extract 3 % in vitro

Daya Hambat ekstrak daun cincau hijau (Cyclea barbata Miers) terhadap pertumbuhan bakteri plak supragingiva (Penelitian Eksperimental laboratories)

Introduction: Dental plaque is an accumulation of thin film on the outer surface of tooth. This mainly consists of microorganisms, most of which are bacteria. There are many ways to prevent the plaque's formation in the tooth surface. Nowadays, herbal therapy is oftenly used as an antibacteria agent to inhibit microorganism's growth. The herb used in this study is green cincau leaf extract (Cyclea barbata Miers). Green cincau leaves contains some active agents which are potential as an antimicrobial such as phenol, flavonoid, (DL)–proto-Quercitol and Tetracontan-19-ol. Therefore the aim of the study is to find out the inhibitory concentration of the green cincau leaves extract (Cyclea barbata Miers) on the growth of supragingiva plaque bacteria. Method: The method used is agar difusion with the concentration of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.5625% and 0.78125%. Result: The results showed that ranging from the concentration of 6.25% occurred a zone of growth inhibitor for the growth of supragingiva plaque bacteria on agar plate. Discussion: Whereas bacterial growth in the concentration of 6.25% above have been inhibited by the green cincau leaves extract.. The conclusion of this study is green cincau leaves extract can be the inhibitory factor for the growth of supragingiva plaque bacteria from the minimum concentration 6.25

MULTIPLE GINGIVAL RECESSION COVERAGE TREATED WITH VISTA TECHNIQUE USING ACELLULAR DERMAL MATRIX (ADM) COMBINED WITH PLATELET RICH FIBRIN (PRF): A CASE REPORT

Background: Gingival recession is an exposure of the root surface due to migration of the marginal periodontal tissues apical to the cemento enamel junction. There are so many root coverage procedures, among these techniques, VISTA technique combined with ADM and PRF offers minimally invasive approach.Case Management: A-40-year-old male visited Periodontics Clinic of DentalHospital Airlangga University with the chief complaint of poor aesthetic resulting from exposed root surfaces in upper right front region of the jaw. Root coverage by VISTA technique using ADM combined with PRF was planned to this case.Discussion: The minimal invasive VISTA technique approach, combined with ADMand PRF has a number of advantages for successful treatment of multiple recession defects. VISTA technique was used because it can maintain better blood supply and maintain critical papillary integrity. ADM works like an autogenous graft by providing a bioactive matrix consisting of collagen, elastin, blood vessel channels, and bioactive protein that support natural revascularization, cell repopulation, and tissue remodeling. Growth factors present in PRF plays a crucial role in hard and soft tissue repair.Conclusion: VISTA technique together with ADM and PRF membrane can besuccessfully used as a treatment method for multiple gingival recessions of Miller’s class I defect

Crude toxin of Aggregatibacter actinomycetemcomitans serotype-B increase PARP-1 expression in gingival epithelium

Background: Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitants) serotype-B has long been associated with aggressive periodontitis. Gingival epithelial cell is exquisitely sensitive to the toxin so that may lead to disruption of the epithelial protective barrier, facilitating invasion and perturbation of the underlying connective tissue. Currently suggested that Aa serotype-B produce protein toxin that caused DNA strand breaks. PARP-1 is an abundant nuclear protein functioning as a DNA nick-sensor enzyme. PARP-1 was one of the first identified substrates of caspases, the main executioners of apoptosis. Therefore, a role for PARP-1 in the regulation of apoptosis has been suggested. Purpose: The purpose of this study was to prove PARP-1 expression in gingival epithelium caused by toxin exposure of A. actinomycetemcomitant serotype-B. Methods: This is an experimental study involving twenty adult mice strain Swiss Webster (balb C) divided randomly into two groups: control group (Group A) and toxin group (Group B). Both group were acclimated for one week before treatment. Group A was applied topically with sterile distillated water every 12 hours. Group B was applied topically by 100μg/ml of crude toxin A. actinomycetemcomitant serotype B at the buccal area of mandibular anterior teeth using Hamilton syringe. The mice were sacrificed at 24 hours after toxin application, and then the tissue sections of gingival epithelium were stained with immunohistochemistry to reveal the PARP-1 expression. The data were analyzed with t-test. Results: The PARP-1 expression exhibited an increase with the toxin group (mean= 48.9; SD= 2.01) compared with the control group (mean= 25.21; SD= 1.72). DNA fragmentation appeared from the agarose gel examination, marked as DNA laddering, indicate the cell apoptosis. Conclusion: In conclusion the crude toxin exposure of A. actinomycetemcomitant serotype-B leads to DNA fragmentation and increase PARP-1 expression

Periodontal Inflamed Surface Area of Patients With Type 2 Diabetes Mellitus Compare with Non-Diabetes Mellitus

Background: Type 2 diabetes mellitus is a significant public health concern. The association between Type 2 diabetes mellitus and periodontal disease is well documented. Currently, a large variety of classifications is used for periodontitis as a risk factor for other diseases. None of these classifications quantifies the amount of inflamed periodontal tissue, while this information is needed to assess the inflammatory burden posed by periodontitis. The author wants to research about periodontal inflamed surface area of patient with type 2 diabetes mellitus compare with non-diabetes mellitus using PISA measurement. Purpose: The purpose of this research is to study the periodontal inflamed surface area of patients with type 2 diabetes mellitus compare with non-diabetes mellitus using PISA measurement. Materials and Method: This research was held in Puskesmas Pucang Sewu Jalan Pucang Anom Timur No.72 Surabaya. Sample is divided in to two groups, 30 patients with type 2 diabetes mellitus and 30 non-diabetes mellitus. Each group is examinated for their blood glucose and underwent full-mouth probing pocket depth and bleeding on probing assessment. The data will be inputted to PISA’s worksheet. Result: The average PISA in patients with type 2 diabetes mellitus is 142,2 mm2, whereas that non-diabetes mellitus is 36,9 mm2. The difference between both group are significant because Mann Whitney test shows p value = 0,00 < 0,05. Conclusion: Periodontal inflamed surface area of patients with type 2 diabetes mellitus is higher than non-diabetes mellitus

Volatile sulphur compounds elimination: A new insight in periodontal treatment

Background: Recent evidences had demonstrated a link between halitosis and apoptosis in periodontitis. Periodontal pathogenic micro-organisms produce volatile sulphur compounds (VSCs). VSCs are toxic to periodontal tissue. Purpose: The purpose of this paper was to reveal the mechanism of VSCs in periodontal breakdown according to the most recent knowledges. r eviews: Halitosis is mainly attributed to VSCs such as hydrogen sulfide, methyl mercaptan and dimethyl sulfide. Several studies demonstrated a strong relationship between VSCs and periodontal disease progression. VSCs are released from amino acid breakdown from food, protein, cells, blood and saliva. In prone subjects, the VSCs may cause alteration in tissue integrity by increasing its permeability and facilitate the endotoxin to penetrate the tissue barrier. They may also causing apoptotic in gingival and periodontal tissue, which are considered the main pathogenesis in aggravating the periodontitis. VSCs may also initiate the increase of proinflammatory cytokines which is considered to have negative effects in host response. Conclusion: VSCs had been shown to have detrimental effects in gingival and periodontal ligament cells. The use of chlorine dioxine agent and topical antioxidant is beneficial in controlling the periodontal disease severity

Keparahan gingivitis pada pasien poli gigi Puskesmas Pacar Keling Surabaya tahun 2016 dengan menggunakan Gingival Index (GI)

Background. Periodontal disease is divide into gingivitis and periodontitis. Gingivitis or gingival inflammation is reversible form of periodontal disease and most commonly found in the oral cavity. Gingivitis clinnically present with a changes in color, contour, consistency, texture and gingival bleeding. Risk assessment can identificated the existance of its severity and periodontal disease. Gingival index is one of the indices that used for assessing the severity of gingival inflamation in patient. Public health service Pacar Keling was selected because availability of comprehensive and reliable data on oral disease based on recognised indicators and there’s no epidemiological data on the severity of gingivitis. Purpose. The aim of this study was to measure the severity of gingivitis at the Dental Section of Public Health Service Pacar Keling Surabaya in 2016. Method. The descriptive observational study with simple random sampling method. This study was performed on 100 patients. The indicator that used for this study was the Gingival Index (GI). Results. Females have higher severity of gingivitis than male. The highest severity of gingivitis of elderly age is in 55-60 years old and subject who had the level of education is in elementary school, brush their teeth one times a day and never check their teeth to dentist, have a higher tendency of severity on gingivitis.Conclusion. The majority of the patients at the Dental Section of Public Health Service Pacar Keling Surabaya in 2016 had moderate gingivitis based on the score of gingival index