Prothymosin α is a component of a linker histone chaperone

AbstractLinker histone H1 binds with high affinity to naked and nucleosomal DNA in vitro but is rapidly exchanged between chromatin sites in vivo suggesting the involvement of one or more linker histone chaperones. Using permeabilized cells, we demonstrate that the small acidic protein prothymosin α (ProTα) can facilitate H1 displacement from and deposition onto the native chromatin template. Depletion of ProTα levels in vivo by siRNA-mediated mRNA degradation resulted in a decreased rate of exchange of linker histones as assayed by photobleaching techniques. These results indicate that ProTα is a component of a linker histone chaperone

Constructed Treatment Wetlands and Water Effect Ratio Study to Achieve Storm Water Compliance at Savannah River Site

2010 S.C. Water Resources Conference - Science and Policy Challenges for a Sustainable Futur

Onshore–Offshore Trends in the Size-Frequency Distribution of Death Assemblages: Northwestern Gulf of Mexico

The size-frequency distributions of death assemblages were compared at three sites on the inner continental shelf of Texas by means of three descriptor variables, numerical abundance, paleoproduction (biomass at death), and paleoingestion (lifetime ingestion, a measure of energy flow). These death assemblages were then compared with six other death assemblages covering a transect from the estuary (Copano Bay, TX) to the continental slope. Typically, size-frequency distributions are based on abundance and size classes are set proportional to the largest individual in the collection. Restriction to this one analysis would have identified few of the important trends observed in this study. The evaluation of size frequency on the basis of species\u27 maximum size as well as assemblage maximum size and the comparison of a suite of assemblages on the basis of the largest maximum size provide important new inferences into community dynamics. The distribution of measures of energy flow across the size-frequency spectrum provided an additional, valuable source of information on community structure and habitat optimality. Within-habitat variability was consistently less than between-habitat variability. The autochthonous continental slope assemblages were the most diverse in their size-frequency spectra. Comparison between habitats showed that the continental slope assemblages had the largest proportion of adult individuals. The continental shelf assemblages were dominated by juveniles. The chemoautotrophic and heterotrophic assemblages in Copano Bay and on the continental slope were similar in most respects despite substantial differences in their trophic structure. Similarity existed in the proportion of adults, in the tendency toward bimodality, and in the degree to which species reached maximum size. The shapes of the size-frequency spectra were controlled in large measure by (a) the relative loss of juveniles through taphonomy, (b) the degree of survivorship to adulthood, probably predominately determined by predation, (c) the food and space resources present that control species size, and (d) the optimality of the habitat that allowed animals to approach maximum size. The habitats on the continental slope had the highest proportion of individuals near maximum size. The Copano Bay assemblages were also characterized by a large proportion of adult individuals; however, these normally did not reach sizes above 70% of species\u27 maximum size. The largest individuals were found at the petroleum seeps and in the heterotrophic assemblages from Copano Bay. Continental slope habitats should be temporally most stable, and our information supports that expectation. Food supply should be greatest in estuaries and in cold seeps where chemosynthetic processes dominate. Our data support this expectation

Maternally sequestered therapeutic polypeptides – a new approach for the management of preeclampsia

The last several decades have seen intensive research into the molecular mechanisms underlying the symptoms of preeclampsia. While the underlying cause of preeclampsia is believed to be defective placental development and resulting placental ischemia, it is only recently that the links between the ischemic placenta and maternal symptomatic manifestation have been elucidated. Several different pathways have been implicated in the development of the disorder; most notably production of the anti-angiogenic protein sFlt-1, induction of auto-immunity and inflammation, and production of reactive oxygen species. While the molecular mechanisms are becoming clearer, translating that knowledge into effective therapeutics has proven elusive. Here we describe a number of peptide based therapies we have developed to target theses pathways, and which are currently being tested in preclinical models. These therapeutics are based on a synthetic polymeric carrier elastin-like polypeptide (ELP), which can be synthesized in various sequences and sizes to stabilize the therapeutic peptide and avoid crossing the placental interface. This prevents fetal exposure and potential developmental effects. The therapeutics designed will target known pathogenic pathways, and the ELP carrier could prove to be a versatile delivery system for administration of a variety of therapeutics during pregnancy

Iron-Line Emission as a Probe of Bardeen-Petterson Accretion Disks

In this work we show that Bardeen-Petterson accretion disks can exhibit unique, detectable features in relativistically broadened emission line profiles. Some of the unique characteristics include inverted line profiles with sharper red horns and softer blue horns and even profiles with more than 2 horns from a single rest-frame line. We demonstrate these points by constructing a series of synthetic line profiles using simple two-component disk models. We find that the resultant profiles are very sensitive to the two key parameters one would like to constrain, namely the Bardeen-Petterson transition radius r_{BP} and the relative tilt \beta between the two disk components over a range of likely values [10 < r_{BP}/(GM/c^2) < 40 ; 15deg < \beta < 45deg]. We use our findings to show that some of the ``extra'' line features observed in the spectrum of the Seyfert-I galaxy MCG--6-30-15 may be attributable to a Bardeen-Petterson disk structure. Similarly, we apply our findings to two likely Bardeen-Petterson candidate Galactic black holes - GRO J1655-40 and XTE J1550-564. We provide synthetic line profiles of these systems using observationally constrained sets of parameters. Although we do not formally fit the data for any of these systems, we confirm that our synthetic spectra are consistent with current observations.Comment: 13 pages, 11 figures, submitted to Ap

The Potential Clinical and Economic Value of Primary Tumour Identification in Metastatic Cancer of Unknown Primary Tumour: A Population-Based Retrospective Matched Cohort Study.

PurposeSeveral genomic tests have recently been developed to identify the primary tumour in cancer of unknown primary tumour (CUP). However, the value of identifying the primary tumour in clinical practice for CUP patients remains questionable and difficult to prove in randomized trials.ObjectiveWe aimed to assess the clinical and economic value of primary tumour identification in CUP using a retrospective matched cohort study.MethodsWe used the Manitoba Cancer Registry to identify all patients initially diagnosed with metastatic cancer between 2002 and 2011. We defined patients as having CUP if their primary tumour was found 6 months or more after initial diagnosis or never found during the course of disease. Otherwise, we considered patients to have metastatic cancer from a known primary tumour (CKP). We linked all patients with Manitoba Health databases to estimate their direct healthcare costs using a phase-of-care approach. We used the propensity score matching technique to match each CUP patient with a CKP patient on clinicopathologic characteristics. We compared treatment patterns, overall survival (OS) and phase-specific healthcare costs between the two patient groups and assessed association with OS using Cox regression adjustment.ResultsOf 5839 patients diagnosed with metastatic cancer, 395 had CUP (6.8%); 1:1 matching created a matched group of 395 CKP patients. CUP patients were less likely to receive surgery, radiation, hormonal and targeted therapy and more likely to receive cytotoxic empiric chemotherapeutic agents. Having CUP was associated with reduced OS (hazard ratio [HR] 1.31; 95% confidence interval 1.1-1.58), but this lost statistical significance with adjustment for treatment differences. CUP patients had a significant increase in the mean net cost of initial diagnostic workup before diagnosis and a significant reduction in the mean net cost of continuing cancer care.ConclusionIdentifying the primary tumour in CUP patients might enable the use of more effective therapies, improve OS and allow more efficient allocation of healthcare resources

Contribution of Extragalactic Infrared Sources to CMB Foreground Anisotropy

We estimate the level of confusion to Cosmic Microwave Background anisotropy measurements caused by extragalactic infrared sources. CMB anisotropy observations at high resolution and high frequencies are especially sensitive to this foreground. We use data from the COBE satellite to generate a Galactic emission spectrum covering mm and sub-mm wavelengths. Using this spectrum as a template, we predict the microwave emission of the 5319 brightest infrared galaxies seen by IRAS. We simulate skymaps over the relevant range of frequencies (30-900 GHz) and instrument resolutions (10'-10 degrees Full Width Half Max). Analysis of the temperature anisotropy of these skymaps shows that a reasonable observational window is available for CMB anisotropy measurements.Comment: 14 pages (LaTex source), 3 PostScript figures. Final version, to appear in ApJLetters May 1. Expanded discussion of systematic error