Identification of bovine leukemia virus tax function associated with host cell transcription, signaling, stress response and immune response pathway by microarray-based gene expression analysis.

BACKGROUND: Bovine leukemia virus (BLV) is associated with enzootic bovine leukosis and is closely related to human T-cell leukemia virus type I. The Tax protein of BLV is a transcriptional activator of viral replication and a key contributor to oncogenic potential. We previously identified interesting mutant forms of Tax with elevated (TaxD247G) or reduced (TaxS240P) transactivation effects on BLV replication and propagation. However, the effects of these mutations on functions other than transcriptional activation are unknown. In this study, to identify genes that play a role in the cascade of signal events regulated by wild-type and mutant Tax proteins, we used a large-scale host cell gene-profiling approach. RESULTS: Using a microarray containing approximately 18,400 human mRNA transcripts, we found several alterations after the expression of Tax proteins in genes involved in many cellular functions such as transcription, signal transduction, cell growth, apoptosis, stress response, and immune response, indicating that Tax protein has multiple biological effects on various cellular environments. We also found that TaxD247G strongly regulated more genes involved in transcription, signal transduction, and cell growth functions, contrary to TaxS240P, which regulated fewer genes. In addition, the expression of genes related to stress response significantly increased in the presence of TaxS240P as compared to wild-type Tax and TaxD247G. By contrast, the largest group of downregulated genes was related to immune response, and the majority of these genes belonged to the interferon family. However, no significant difference in the expression level of downregulated genes was observed among the Tax proteins. Finally, the expression of important cellular factors obtained from the human microarray results were validated at the RNA and protein levels by real-time quantitative reverse transcription-polymerase chain reaction and western blotting, respectively, after transfecting Tax proteins into bovine cells and human HeLa cells. CONCLUSION: A comparative analysis of wild-type and mutant Tax proteins indicates that Tax protein exerts a significant impact on cellular functions as diverse as transcription, signal transduction, cell growth, stress response and immune response. Importantly, our study is the first report that shows the extent to which BLV Tax regulates the innate immune response

Boundary-element calculations for amplification of effects of low-frequency electric fields in a doublet-shaped biological cell

金沢大学医薬保健研究域保健学系To examine the amplification of the effects of low-frequency electric fields due to the junction between the cells, the amplitude of transmembrane potential PTMP0, the time-averaged normal stress σn and the outward force density σout on the shell-phase, and the squared intensity Iee of electric fields in the inner and the outer phases induced by uniform external ac fields were calculated with the boundary-element method for doublet-shaped cell models consisting of two spheres of the same size connected by the junction; the results were compared with those in a spherical model. When the external fields were parallel to the long axis of the doublet-shaped models, PTMP0, σn and σout at the pole were greater than those in the spherical model, and σout and Iee at the junction increased with the decrease in the junction-radius. The external fields perpendicular to the long axis caused Iee greater than that at the center of the spherical model and negative σout, at the junction. The amplification of PTMP0, σn, σout and Iee took place within restricted frequency-regions that could be specified by the characteristic frequencies for the frequency-dependence of the polarization factor of the models. © 2009 Elsevier B.V. All rights reserved

Substellar Companions to Evolved Intermediate-Mass Stars: HD 145457 and HD 180314

We report the detections of two substellar companions orbiting around evolved intermediate-mass stars from precise Doppler measurements at Subaru Telescope and Okayama Astrophysical Observatory. HD 145457 is a K0 giant with a mass of 1.9 M_sun and has a planet of minimum mass m_2sini=2.9 M_J orbiting with period of P=176 d and eccentricity of e=0.11. HD 180314 is also a K0 giant with 2.6 M_sun and hosts a substellar companion of m_2sin i=22 M_J, which falls in brown-dwarf mass regime, in an orbit with P=396 d and e=0.26. HD 145457 b is one of the innermost planets and HD 180314 b is the seventh candidate of brown-dwarf-mass companion found around intermediate-mass evolved stars.Comment: 16 pages, 4 figures, accepted for publication in PAS

Five Intermediate-Period Planets from the N2K Sample

We report the detection of five Jovian mass planets orbiting high metallicity stars. Four of these stars were first observed as part of the N2K program and exhibited low RMS velocity scatter after three consecutive observations. However, follow-up observations over the last three years now reveal the presence of longer period planets with orbital periods ranging from 21 days to a few years. HD 11506 is a G0V star with a planet of \msini = 4.74 \mjup in a 3.85 year orbit. HD 17156 is a G0V star with a 3.12 \mjup planet in a 21.2 day orbit. The eccentricity of this orbit is 0.67, one of the highest known for a planet with a relatively short period. The orbital period for this planet places it in a region of parameter space where relatively few planets have been detected. HD 125612 is a G3V star with a planet of \msini = 3.5 \mjup in a 1.4 year orbit. HD 170469 is a G5IV star with a planet of \msini = 0.67 \mjup in a 3.13 year orbit. HD 231701 is an F8V star with planet of 1.08 \mjup in a 142 day orbit. All of these stars have supersolar metallicity. Three of the five stars were observed photometrically but showed no evidence of brightness variability. A transit search conducted for HD 17156 was negative but covered only 25% of the search space and so is not conclusive.Comment: 13 pages, 9 figures, accepted ApJ Resubmitted here with some additional data, modified Keplerian orbit

Planetary Companions to Evolved Intermediate-Mass Stars: 14 Andromedae, 81 Ceti, 6 Lyncis, and HD 167042

We report on the detection of four extrasolar planets orbiting evolved intermediate-mass stars from a precise Doppler survey of G and K giants at Okayama Astrophysical Observatory. All of the host stars are considered to be formerly early F-type or A-type dwarfs when they were on the main sequence. 14 And (K0 III) is a clump giant with a mass of 2.2 M_solar and has a planet of minimum mass m_2sin i=4.8 M_Jup in a nearly circular orbit with a 186 day period. This is one of the innermost planets around evolved intermediate-mass stars and such planets have only been discovered in clump giants. 81 Cet (G5 III) is a clump giant with 2.4 M_solar hosting a planet of m_2sin i=5.3 M_Jup in a 953 day orbit with an eccentricity of e=0.21. 6 Lyn (K0 IV) is a less evolved subgiant with 1.7 M_solar and has a planet of m_2sin i=2.4 M_Jup in a 899 day orbit with e=0.13. HD 167042 (K1 IV) is also a less evolved star with 1.5 M_solar hosting a planet of m_2sin i=1.6 M_Jup in a 418 day orbit with e=0.10. This planet was independently announced by Johnson et al. (2008, ApJ, 675, 784). All of the host stars have solar or sub-solar metallicity, which supports the lack of metal-rich tendency in planet-harboring giants in contrast to the case of dwarfs.Comment: 23 pages, 6 figures, accepted for publication in PAS

A Substellar Companion to the Intermediate-Mass Giant 11 Com

We report the detection of a substellar companion orbiting the intermediate-mass giant star 11 Com (G8 III). Precise Doppler measurements of the star from Xinglong station and Okayama Astrophysical Observatory (OAO) revealed Keplerian velocity variations with an orbital period of 326.03 +/- 0.32 days, a semiamplitude of 302.8 +/- 2.6 m/s, and an eccentricity of 0.231 +/- 0.005. Adopting a stellar mass of 2.7 +/- 0.3 M_solar, the minimum mass of the companion is 19.4 +/- 1.5 M_Jup, well above the deuterium burning limit, and the semimajor axis is 1.29 +/- 0.05 AU. This is the first result from the joint planet search program between China and Japan aiming at revealing statistics of substellar companions around intermediate-mass giants. 11 Com b emerged from 300 targets of the planet search program at OAO. The current detection rate of a brown dwarf candidate seems to be comparable to that around solar-type stars within orbital separations of $\sim$3 AU.Comment: 19 pages, 4 figures, accepted by Ap