315 research outputs found
BAFF controls B cell metabolic fitness through a PKCβ- and Akt-dependent mechanism
B cell life depends critically on the cytokine B cell–activating factor of the tumor necrosis factor family (BAFF). Lack of BAFF signaling leads to B cell death and immunodeficiency. Excessive BAFF signaling promotes lupus-like autoimmunity. Despite the great importance of BAFF to B cell biology, its signaling mechanism is not well characterized. We show that BAFF initiates signaling and transcriptional programs, which support B cell survival, metabolic fitness, and readiness for antigen-induced proliferation. We further identify a BAFF-specific protein kinase C β–Akt signaling axis, which provides a connection between BAFF and generic growth factor–induced cellular responses
Cytosol-derived proteins are sufficient for Arp2/3 recruitment and ARF/coatomer-dependent actin polymerization on Golgi membranes
AbstractThe actin cytoskeleton has been implicated in protein trafficking at the Golgi apparatus and in Golgi orientation and morphology. Actin dynamics at the Golgi are regulated in part by recruiting Cdc42 or Rac to the membrane through a binding interaction with the coatomer-coated (COPI)-vesicle coat protein, coatomer. This leads to actin polymerization through the effector, N-WASP and the Arp2/3 complex. Here, we have used reconstitution of vesicle budding to test whether Arp2/3 is recruited to membranes during the formation of COPI vesicles. Our results revealed that ARF1 activation leads to greatly increased Arp3 levels on the membranes. Coatomer-bound Cdc42 and pre-existing F-actin are important for Arp2/3 binding. ARF1-dependent Arp2/3 recruitment and actin polymerization can be reconstituted on liposomal membranes, indicating that no membrane proteins are necessary. These results show that activated ARF1 can stimulate Arp2/3 recruitment to Golgi membranes through coatomer, Cdc42 or Rac, and N-WASP
Nab2p and the Thp1p-Sac3p Complex Functionally Interact at the Interface between Transcription and mRNA Metabolism
THP1 is a conserved eukaryotic gene whose null mutations confer, in yeast, transcription and genetic instability phenotypes and RNA export defects similar to those of the THO/TREX complex null mutations. In a search for multicopy suppressors of the transcription defect of thp1Δ cells, we identified the poly(A)+ RNA-binding heterogeneous nuclear ribonucleoprotein Nab2p. Multicopy NAB2 also suppressed the RNA export defect of thp1Δ cells. This result suggests a functional relationship between Thp1p and Nab2p. Consistently, the leaky mutation nab2-1 conferred a transcription defect and hyper-recombination phenotype similar to those of thp1Δ, although to a minor degree. Reciprocally, a purified His6-tagged Thp1p fusion bound RNA in vitro. In a different approach, we show by Western analyses that a highly purified Thp1p-Sac3p complex does not contain components of THO/TREX and that sac3Δ confers a transcription defect and hyper-recombination phenotype identical to those of thp1Δ. mRNA degradation was not affected in thp1Δ mutants, implying that their expression defects are not due to mRNA decay. This indicates that Thp1p-Sac3p is a structural and functional unit. Altogether, our results suggest that Thp1p-Sac3p and Nab2p are functionally related heterogeneous nuclear ribonucleoproteins that define a further link between mRNA metabolism and transcription
Suppression of mitochondrial respiration through recruitment of p160 myb binding protein to PGC-1α : modulation by p38 MAPK
The transcriptional coactivator PPAR gamma coactivator 1 α (PGC-1α) is a key regulator of metabolic processes such as mitochondrial biogenesis and respiration in muscle and gluconeogenesis in liver. Reduced levels of PGC-1α in humans have been associated with type II diabetes. PGC-1α contains a negative regulatory domain that attenuates its transcriptional activity. This negative regulation is removed by phosphorylation of PGC-1α by p38 MAPK, an important kinase downstream of cytokine signaling in muscle and β-adrenergic signaling in brown fat. We describe here the identification of p160 myb binding protein (p160MBP) as a repressor of PGC-1α. The binding and repression of PGC-1α by p160MBP is disrupted by p38 MAPK phosphorylation of PGC-1α. Adenoviral expression of p160MBP in myoblasts strongly reduces PGC-1α's ability to stimulate mitochondrial respiration and the expression of the genes of the electron transport system. This repression does not require removal of PGC-1α from chromatin, suggesting that p160MBP is or recruits a direct transcriptional suppressor. Overall, these data indicate that p160MBP is a powerful negative regulator of PGC-1α function and provide a molecular mechanism for the activation of PGC-1α by p38 MAPK. The discovery of p160MBP as a PGC-1α regulator has important implications for the understanding of energy balance and diabetes
Coatomer-bound Cdc42 regulates dynein recruitment to COPI vesicles
Cytoskeletal dynamics at the Golgi apparatus are regulated in part through a binding interaction between the Golgi-vesicle coat protein, coatomer, and the regulatory GTP-binding protein Cdc42 (Wu, W.J., J.W. Erickson, R. Lin, and R.A. Cerione. 2000. Nature. 405:800–804; Fucini, R.V., J.L. Chen, C. Sharma, M.M. Kessels, and M. Stamnes. 2002. Mol. Biol. Cell. 13:621–631). The precise role of this complex has not been determined. We have analyzed the protein composition of Golgi-derived coat protomer I (COPI)–coated vesicles after activating or inhibiting signaling through coatomer-bound Cdc42. We show that Cdc42 has profound effects on the recruitment of dynein to COPI vesicles. Cdc42, when bound to coatomer, inhibits dynein binding to COPI vesicles whereas preventing the coatomer–Cdc42 interaction stimulates dynein binding. Dynein recruitment was found to involve actin dynamics and dynactin. Reclustering of nocodazole-dispersed Golgi stacks and microtubule/dynein-dependent ER-to-Golgi transport are both sensitive to disrupting Cdc42 mediated signaling. By contrast, dynein-independent transport to the Golgi complex is insensitive to mutant Cdc42. We propose a model for how proper temporal regulation of motor-based vesicle translocation could be coupled to the completion of vesicle formation
The human PAF complex coordinates transcription with events downstream of RNA synthesis.
The yeast PAF (yPAF) complex interacts with RNA polymerase II and coordinates the setting of histone marks associated with active transcription. We report the isolation and functional characterization of the human PAF (hPAF) complex. hPAF shares four subunits with yPAF (hCtr9, hPaf1, hLeo1, and hCdc73), but contains a novel higher eukaryotic-specific subunit, hSki8. RNAi against hSki8 or hCtr9 reduces the cellular levels of other hPAF subunits and of mono- and trimethylated H3-Lys 4 and dimethylated H3-Lys 79. The hSki8 subunit is also a component of the human SKI (hSKI) complex. Yeast SKI complex is cytoplasmic and together with Exosome mediates 3\u27-5\u27 mRNA degradation. However, hSKI complex localizes to both nucleus and cytoplasm. Immunoprecipitation experiments revealed that hPAF and hSKI complexes interact, and ChIP experiments demonstrated that hSKI associates with transcriptionally active genes dependent on the presence of hPAF. Thus, in addition to coordinating events during transcription (initiation, promoter clearance, and elongation), hPAF also coordinates events in RNA quality control
Highly efficient selenomethionine labeling of recombinant proteins produced in mammalian cells
Abstract The advent of the multiwavelength anomalousdiffraction phasing methodhas significantlyacceleratedcrystal structuredeterminationand hasbecomethe norm in protein crystallography. This method allowsresearchersto takeadvantageofthe anomalous signal fromdivers eatoms,but thedominantmethod forderivativ epreparation is selenomethionine substitution.S everal generallya pplicable, high-efficiency labeling protocolsh aveb een developed for use in the bacterial, yeast, andbaculovirus/insect cell expressionsystems butnot formammalian tissuec ulture.A sal arge number of proteinso fb iomedical importance cano nlyb ep roducedi ny ields sufficient forX -ray diffraction experimentsi nm ammalian expression systems, it becomesa ll them ore importantt od evelops uchp rotocols.W et herefore evaluateds everal variablest hatp lay roles in determining incorporation levels andr eporth ere as imple protocolf or selenom ethionine modification of proteinsi n mammalian cellsr outinely yielding >90%l abeling efficiency. Keywords: proteinl abeling; proteinc rystallography; selenomethionine; multiplew avelength anomalous diffraction; mammalian cell culture The multiwavelength anomalous diffraction (MAD)phasingm ethod (Hendrickson1 991) hasb ecome them ethod of choice for X-ray phase determination, with >50% of thee xperimentally phased structures deposited in the PDB during thep asty ear beingd etermined by MAD. WhileM AD has allowed researchers to take advantage of thea nomalouss ignal from several diverse heavy atoms, thed ominantm ethodf or heavya tomd erivative preparation is selenomethioninesubstitution. Several factors contribute to the widespread use of selenomethionine substitution, including simplicity, adaptabilitytodifferent expression systems, scalability,a nd, in some cases, an almost quantitative replacement of methionine resulting in ahomogeneousprotein population. This method results in modified proteins withoutsignificantstructural perturbations due to heavy atom incorporation,w hile eliminatingthe difficult and time-consuming screenings forheavy atom derivatives. It is estimated that, foras uccessful MADe xperiment, ones elenomethionine residue is required for every ; 75-100a mino acids (Hendrickson andO gata 1997). This corresponds to ; 80%o fa ll proteins, which have am ethioninecontentof1%ormore (Strub et al.2003). There are twol imitations to the method: First, the calculations abovea ssumeq uantitative( or near-quantitative) methionine substitution, which often is not the case.F or example, as the complexity of the expression system host increases, so does the complexity of the media requiredfor their growth, Article published onlinea head of print. Articlea nd publication date are at http://www.proteinscience.org/cg
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