Gökkuşağı alabalığı (Oncorhynchus mykiss Walbaum, 1792)'nda curcuminin bazı antioksidan parametreler üzerine etkisi

Bu çalışmada; curcuminin gökkuşağı alabalığı (Oncorhynchus mykiss)'nda bazı antioksidan parametrelere etkisi incelendi. Curcuminin 10, 20 ve 40 mg/kg yem miktarları 21 gün süreyle balıklara verildi. Deneme sonunda balıklardan karaciğer, böbrek ve dalak örnekleri alındı. Bu dokularda malondialdehit (MDA) ve redükte glutatyon (GSH) düzeyi ile glutatyon peroksidaz (GSH-Px), glutatyon redüktaz (GR) ve glutatyon S-transferaz (GST) enzim aktiviteleri incelendi. Curcumin uygulanan balıklarda MDA düzeyinin düştüğü, GSH-Px, GR ve GST aktiviteleri ile GSH düzeyinin ise arttığı belirlendi (p<0.05).In this study, the effect of curcumin on some antioxidant parameters of rainbow trout (Oncorhynchus mykiss) were examined. The amounts of 10, 20 and 40 mg curcumin for per kg diet were given to fish for 21 days. At the end of this period, liver, kidney and spleen samples were taken from the fish. Malondialdehyde (MDA) and reduce glutathione (GSH) level, glutathione peroxidase (GSH-Px), glutathione reductase (GR) and glutathione S transferase (GST) enzyme activities were investigated in these tissues. It was found that the MDA level decreased, while the GSH-Px, GR and GST enzyme activities and the GSH level increased in the fish applied curcumin (p<0.05)

Investigation of Some Immunological Parameters in Rainbow Trout (Oncorhynchus mykiss) Injected Propolis

In this study, effects of propolis on immunological parameters of rainbow trout were investigated. For this purpose, propolis was intraperitoneally injected to fish 4 times at doses of 2.5, 5 and 10 mg / kg fish weight. Blood samples on 3, 9, 15 and 21 days were collected from fish of the control and experimental groups and analysed to determine oxidative radical production [nitroblue tetrazolium (NBT) activity], total plasma protein and total immunoglobulin levels. At the end of the experiment, there was a statistically significant increase in oxidative radical production, total protein and immunoglobulin levels of propolis treated groups when compared to the control group

Ameliorative Effect of Lycopene on Haematological Indices of Common Carp Cyprinus carpio, Linnaeus, 1758 Exposed to Cypermethrin

The present study was conducted to investigate the effectiveness of lycopene in alleviating the toxicity of cypermethrin (CYP) on haematological parameters in carp, Cyprinus carpio. Fish (totally 140 fish) were exposed to sublethal concentrations (0.202 and 0.404 μg/L) of CYP, and lycopene (10 mg per kg of fish weight) was simultaneously administered. At the end of 28 days administration, blood samples were collected and haematological changes (red and white blood cell count, haemoglobin concentration, haematocrit level, and erythrocyte indices: mean corpuscular volume, mean corpuscular haemoglobin, and mean corpuscular haemoglobin concentration) were determined. According to the data obtained, CYP was determined to lead to negative alterations in the haematological parameters investigated. The administration of lycopene alleviated this effect

Propolisin Genel Özellikleri ve Balıklarda Kullanımı

Propolis, bitkilerin tomurcuk ve yaprak kısımlarından toplanan maddelerden bal arıları tarafından üretilen doğal reçineli bir karışımdır. Propolis antikanser, antiinflamator, antibiyotik, antioksidatif, antibakteriyel, antiviral, antifungal, anestetik, immunostimulan ve sitotoksik etkilere sahiptir. Bu derlemede doğal bir ürün olan propolisin fiziksel, kimyasal ve biyolojik özellikleri, bitkisel kaynakları ile balıklarda kullanımı ve uygulama şekiller hakkındaki bilgilerin bir araya getirilmesi amaçlanmıştır

Protective effect of propolis against oxidative stress and immunosuppression induced by oxytetracycline in rainbow trout (Oncorhynchus mykiss, W.)

The aim of this study was to investigate the effects of propolis on oxytetracycline (OTC)-induced oxidative stress and immunosuppression in fish. OTC (100 mg per kg(-1) body weight) was orally administered to fish for 14 days. A significant elevation in the level of malondialdehyde, as an index of lipid peroxidation, and reductions in antioxidant enzyme activities (superoxide dismutase, catalase, and glutathione peroxidase) and low molecular weight antioxidant (reduced glutathione) levels were observed in the blood, liver, kidney, spleen, and heart tissues of OTC-treated fish. OTC also had a suppressive effect on specific and non-specific immune system parameters, such as leucocyte counts, oxidative radical production (nitrobluetetrazolium activity), total plasma protein and immunoglobulin levels, and phagocytic activity. Pre-treatment, post-treatment, and simultaneous treatment with propolis (50 mg per kg(-1) body weight, orally) attenuated the OTC-induced oxidative stress by significantly decreasing the levels of malondialdehyde in tissues. In addition, propolis significantly increased the level of reduced glutathione and the catalase, glutathione peroxidase, and superoxide dismutase activities. Upon the administration of propolis, the suppressed immune system parameters were significantly increased in fish treated with OTC The present results suggest that pretreatment, post-treatment, and simultaneous administration of propolis might alleviate OTC-induced oxidative stress and immunosuppression. (C) 2011 Elsevier Ltd. All rights reserved