Generation of Tunable Narrow Bandwidth Nanosecond Pulses in the Deep Ultraviolet for Efficient Optical Pumping and High Resolution Spectroscopy

Nanosecond optical pulses with high power and spectral brightness in the deep ultraviolet (UV) region have been produced by sum frequency mixing of nearly transform-limited-bandwidth IR light originating from a home-built injection-seeded ring cavityoptical parametric oscillator(OPO) and the fourth harmonic beam of an injection-seeded Nd:YAG laser used simultaneously to pump the OPO with the second harmonic. We demonstrate UV output, tunable from 204 to 207 nm, which exhibits pulse energies up to 5 mJ with a bandwidth better than [Math Processing Error]. We describe how the approach shown in this paper can be extended to wavelengths shorter than 185 nm. The injection-seeded OPO provides high conversion efficiency ([Math Processing Error] overall energy conversion) and superior beam quality required for highly efficient downstream mixing where sum frequencies are generated in the UV. The frequency stability of the system is excellent, making it highly suitable for optical pumping. We demonstrate high resolution spectroscopy as well as optical pumping using laser-induced fluorescence and stimulated emission pumping, respectively, in supersonic pulsed molecular beams of nitric oxide

Generation of Tunable Narrow Bandwidth Nanosecond Pulses in the Deep Ultraviolet for Efficient Optical Pumping and High Resolution Spectroscopy

Nanosecond optical pulses with high power and spectral brightness in the deep ultraviolet (UV) region have been produced by sum frequency mixing of nearly transform-limited-bandwidth IR light originating from a home-built injection-seeded ring cavityoptical parametric oscillator(OPO) and the fourth harmonic beam of an injection-seeded Nd:YAG laser used simultaneously to pump the OPO with the second harmonic. We demonstrate UV output, tunable from 204 to 207 nm, which exhibits pulse energies up to 5 mJ with a bandwidth better than [Math Processing Error]. We describe how the approach shown in this paper can be extended to wavelengths shorter than 185 nm. The injection-seeded OPO provides high conversion efficiency ([Math Processing Error] overall energy conversion) and superior beam quality required for highly efficient downstream mixing where sum frequencies are generated in the UV. The frequency stability of the system is excellent, making it highly suitable for optical pumping. We demonstrate high resolution spectroscopy as well as optical pumping using laser-induced fluorescence and stimulated emission pumping, respectively, in supersonic pulsed molecular beams of nitric oxide

Trends in incidence, mortality, and causes of death associated with systemic sclerosis in Denmark between 1995 and 2015:a nationwide cohort study

Variables and sources. Diagnoses (ICD-8 and ICD-10) and medication (ATC, Anatomical Therapeutical Chemical) codes used. (DOCX 20 kb

Inception of a global atlas of sea levels since the Last Glacial Maximum

Determining the rates, mechanisms, and geographic variability of relative sea-level (RSL) change following the Last Glacial Maximum (LGM) provides insight into the sensitivity of ice sheets to climate change, the response of the solid Earth and gravity field to ice-mass redistribution, and constrains statistical and physical models used to project future sea-level rise. To do so in a scientifically robust way requires standardized datasets that enable broad spatial comparisons that minimize bias. As part of a larger goal to develop a unified, spatially-comprehensive post-LGM global RSL database, in this special issue we provide a standardized global synthesis of regional RSL data that resulted from the first ‘Geographic variability of HOLocene relative SEA level (HOLSEA)’ meetings in Mt Hood, Oregon (2016) and St Lucia, South Africa (2017). The HOLSEA meetings brought together sea-level researchers to agree upon a consistent protocol to standardize, interpret, and incorporate realistic uncertainties of RSL data. This special issue provides RSL data from ten geographical regions including new databases from Atlantic Europe and the Russian Arctic and revised/expanded databases from Atlantic Canada, the British Isles, the Netherlands, the western Mediterranean, the Adriatic, Israel, Peninsular Malaysia, Southeast Asia, and the Indian Ocean. In total, the database derived from this special issue includes 5634 (5290 validated) index (n = 3202) and limiting points (n = 2088) that span from ∼20,000 years ago to present. Progress in improving the standardization of sea-level databases has also been accompanied by advancements in statistical and analytical methods used to infer spatial patterns and rates of RSL change from geological data that have a spatially and temporally sparse distribution and geochronological and elevational uncertainties. This special issue marks the inception of a unified, spatially-comprehensive post-LGM global RSL database

Measurement of the ambient organic aerosol volatility distribution: application during the Finokalia Aerosol Measurement Experiment (FAME-2008)

A variable residence time thermodenuder (TD) was combined with an Aerodyne Aerosol Mass Spectrometer (AMS) and a Scanning Mobility Particle Sizer (SMPS) to measure the volatility distribution of aged organic aerosol in the Eastern Mediterranean during the Finokalia Aerosol Measurement Experiment in May of 2008 (FAME-2008). A new method for the quantification of the organic aerosol volatility distribution was developed combining measurements of all three instruments together with an aerosol dynamics model. <br><br> Challenges in the interpretation of ambient thermodenuder-AMS measurements include the potential resistances to mass transfer during particle evaporation, the effects of particle size on the evaporated mass fraction, the changes in the AMS collection efficiency and particle density as the particles evaporate partially in the TD, and finally potential losses inside the TD. Our proposed measurement and data analysis method accounts for all of these problems combining the AMS and SMPS measurements. <br><br> The AMS collection efficiency of the aerosol that passed through the TD was found to be approximately 10% lower than the collection efficiency of the aerosol that passed through the bypass. The organic aerosol measured at Finokalia is approximately 2 or more orders of magnitude less volatile than fresh laboratory-generated monoterpene (α-pinene, β-pinene and limonene under low NO<sub>x</sub> conditions) secondary organic aerosol. This low volatility is consistent with its highly oxygenated AMS mass spectrum. The results are found to be highly sensitive to the mass accommodation coefficient of the evaporating species. This analysis is based on the assumption that there were no significant reactions taking place inside the thermodenuder

Aged organic aerosol in the Eastern Mediterranean: the Finokalia Aerosol Measurement Experiment – 2008

Aged organic aerosol (OA) was measured at a remote coastal site on the island of Crete, Greece during the Finokalia Aerosol Measurement Experiment-2008 (FAME-2008), which was part of the EUCAARI intensive campaign of May 2008. The site at Finokalia is influenced by air masses from different source regions, including long-range transport of pollution from continental Europe. A quadrupole aerosol mass spectrometer (Q-AMS) was employed to measure the size-resolved chemical composition of non-refractory submicron aerosol (NR-PM<sub>1</sub>), and to estimate the extent of oxidation of the organic aerosol. Factor analysis was used to gain insights into the processes and sources affecting the OA composition. The particles were internally mixed and liquid. The largest fraction of the dry NR-PM<sub>1</sub> sampled was ammonium sulfate and ammonium bisulfate, followed by organics and a small amount of nitrate. The variability in OA composition could be explained with two factors of oxygenated organic aerosol (OOA) with differing extents of oxidation but similar volatility. Hydrocarbon-like organic aerosol (HOA) was not detected. There was no statistically significant diurnal variation in the bulk composition of NR-PM<sub>1</sub> such as total sulfate or total organic aerosol concentrations. However, the OA composition exhibited statistically significant diurnal variation with more oxidized OA in the afternoon. The organic aerosol was highly oxidized, regardless of the source region. Total OA concentrations also varied little with source region, suggesting that local sources had only a small effect on OA concentrations measured at Finokalia. The aerosol was transported for about one day before arriving at the site, corresponding to an OH exposure of approximately 4×10<sup>11</sup> molecules cm<sup>−3</sup> s. The constant extent of oxidation suggests that atmospheric aging results in a highly oxidized OA at these OH exposures, regardless of the aerosol source

Fluorescence characterization of clinically-important bacteria

Healthcare-associated infections (HCAI/HAI) represent a substantial threat to patient health during hospitalization and incur billions of dollars additional cost for subsequent treatment. One promising method for the detection of bacterial contamination in a clinical setting before an HAI outbreak occurs is to exploit native fluorescence of cellular molecules for a hand-held, rapid-sweep surveillance instrument. Previous studies have shown fluorescence-based detection to be sensitive and effective for food-borne and environmental microorganisms, and even to be able to distinguish between cell types, but this powerful technique has not yet been deployed on the macroscale for the primary surveillance of contamination in healthcare facilities to prevent HAI. Here we report experimental data for the specification and design of such a fluorescence-based detection instrument. We have characterized the complete fluorescence response of eleven clinically-relevant bacteria by generating excitation-emission matrices (EEMs) over broad wavelength ranges. Furthermore, a number of surfaces and items of equipment commonly present on a ward, and potentially responsible for pathogen transfer, have been analyzed for potential issues of background fluorescence masking the signal from contaminant bacteria. These include bedside handrails, nurse call button, blood pressure cuff and ward computer keyboard, as well as disinfectant cleaning products and microfiber cloth. All examined bacterial strains exhibited a distinctive double-peak fluorescence feature associated with tryptophan with no other cellular fluorophore detected. Thus, this fluorescence survey found that an emission peak of 340nm, from an excitation source at 280nm, was the cellular fluorescence signal to target for detection of bacterial contamination. The majority of materials analysed offer a spectral window through which bacterial contamination could indeed be detected. A few instances were found of potential problems of background fluorescence masking that of bacteria, but in the case of the microfiber cleaning cloth, imaging techniques could morphologically distinguish between stray strands and bacterial contamination