Individual adaptation of industry LCA practice: Results from two case studies in the Swedish forest products industry

Goal, Scope and Background: The mere existence of life cycle assessment (LCA) methodology and general acceptance of the life cycle philosophy is not enough to make their use widespread in industry. To gain a better understanding of factors shaping LCA studies and life cycle related practice, field studies of the development of LCA practice in two companies were carried out. Methods: In order to obtain a deeper understanding of LCA practice, the number of ‘variables’ was minimized and two similar companies were chosen for study: Stora Enso and SCA. Both companies are part of the Swedish forest products industry, are large multinational enterprises and have been working with LCA since the early 1990s. Both interviews and document studies were used to collect data regarding LCA work from its introduction until 2003. Results and Discussion: We found fundamental differences in LCA practice between two similar companies in regard to LCA studies per se (the number of studies undertaken and methodological preferences) and also in regard to the organisation of and approach to LCA work. By testing various theoretical explanations of these divergent LCA practices, we identified the actions of individuals and their understanding of the situation as important factors shaping LCA practice. Conclusions: Although sector-wide recommendations on LCA practice are common in the LCA community, this study indicates that companies use LCA differently despite similar structural conditions such as company size or sector affiliation. Recommendations and Perspectives: Since the understanding and actions of individuals are important in shaping LCA practice, people working with LCA in industry probably have greater scope for action than they recognise and than sector recommendations may imply when it comes to organising and carrying out their work. Thus, those working with life cycle issues, even in different sectors, can learn much from each other about ways of organising and benefiting from LCA work

Recommendation and context: the missing links for increased life cycle impact in large industries

PurposeThis study takes an open and explorative approach to investigating the impact, or lack of impact, of life cycle information on behaviours throughout large production companies. Based on cases where life cycle information has been provided, this paper analyses how life cycle information has been interpreted and acted upon—not only by the life cycle assessment (LCA) practitioner conducting the study but also by employees outside the environmental departments.MethodsTo understand the impact of life cycle information on everyday actions in organisations and how this impact can be enhanced, this study takes a grounded approach to following flows of life cycle information from the environmental department through other departments of an organisation. From the flows of information, the research team selected rich descriptions of empirical data that reflect action and inaction. Using interviews and documents, we collected barriers and enablers for acting on life cycle information. Barriers and enablers were interpreted and clustered into categories and arranged into concepts. Next, we reviewed the empirical data using theories from social psychology.Results and discussionThe results show that it is difficult for life cycle information to result in subsequent action outside of environmental departments. The barriers to this action were partly due to the life cycle information per se such as gaps between what life cycle information is available and what life cycle information is needed. Barriers and enablers were also found in relation to the context in which life cycle information was applied and new behaviours were adopted, including timing and software structures, reward systems, trade-offs, and personal beliefs about the profession. The results suggest a new role of the life cycle proponent that includes providing the right life cycle information and understanding and influencing the expected agents’ situations.ConclusionsAssisted by theories from social psychology, we found that behaviour can be changed if ‘recommendations’ and ‘contexts’ are considered when providing life cycle information. The paper suggests that the impact of life cycle information could increase if normative arguments about environmental visions, strategies, and overarching goals are aligned with enablers that focus on personal goals, such as meeting a deadline, reducing uncertainty, and reaching the threshold for a bonus

LCM development: Focusing on the LC promoters and their organizational problem-solving

Purpose:\ua0Life cycle management (LCM) implies a specific sustainability perspective which extends environmental managementalong the product life cycle, with the aim of decreasing negative environmental impact throughout the product chain. Researchhas identified that the adoption of LCMin the industry depends upon its situational adaptation to the organizational context. Evenso, little is known about the specifics of this adaptation. With this paper, our aim is to add knowledge on LCM adoption andadaptation.Methods: A systematic analysis of empirical material on life cycle (LC) activity in six multinational corporations (MNCs) isconducted, by applying a secondary analysis of qualitative data (Heaton 2008). In order to study instances of LCM adoption andadaptation, we focus on the acts and situations of LC promoters. The identified instances are analyzed through the lens of situatedproblem-solving (Kuhn and Jackson 2008).Results and discussion: Sixty-seven instances of LC promotion were identified and analyzed, resulting in the identification ofeight categories of problem-situations typically encountered by LC promoters. The identified problem-situations representdifferent situations when the organizational appropriateness of the LC approach is at stake and to which responses tailored tothe organization are put forward by a LC promoter. The results bring to the fore the ubiquity of organizational and creativeproblem-solving, highlighting the role of LC promoters as change agents for LCMadoption, and depict the development of LCMas an emergent practice, rather than an implementation process.Conclusions: This paper provides a first systematic analysis of LC promoters enacting a variety of responses to organizationallychallenging LC situations, thus detailing the adaptation necessary for embedding LCM in the industry. Findings show that thedevelopment of LCM to a great extent is about the promotion of a LC approach, and that LC promoters need organizationalknowing, in addition to LC knowing, to make the LC approach relevant to management and business

Deregulation of the endometrial stromal cell secretome precedes embryo implantation failure

STUDY QUESTION Is implantation failure following ART associated with a perturbed decidual response in endometrial stromal cells (EnSCs)? SUMMARY ANSWER Dynamic changes in the secretome of decidualizing EnSCs underpin the transition of a hostile to a supportive endometrial microenvironment for embryo implantation; perturbation in this transitional pathway prior to ART is associated with implantation failure. WHAT IS KNOWN ALREADY Implantation is the rate-limiting step in ART, although the contribution of an aberrant endometrial microenvironment in IVF failure remains ill defined. STUDY DESIGN, SIZE, DURATION In vitro characterization of the temporal changes in the decidual response of primary EnSCs isolated prior to a successful or failed ART cycle. An analysis of embryo responses to secreted cues from undifferentiated and decidualizing EnSCs was performed. The primary clinical outcome of the study was a positive urinary pregnancy test 14 days after embryo transfer. PARTICIPANTS/MATERIALS, SETTING, METHODS Primary EnSCs were isolated from endometrial biopsies obtained prior to IVF treatment and cryopreserved. EnSCs from 10 pregnant and 10 non-pregnant patients were then thawed, expanded in culture, subjected to clonogenic assays, and decidualized for either 2 or 8 days. Transcript levels of decidual marker gene [prolactin (PRL), insulin-like growth factor binding protein 1 (IGFBP1) and 11β-hydroxysteroid dehydrogenase (HSD11B1)] were analysed using real-time quantitative PCR and temporal secretome changes of 45 cytokines, chemokines and growth factors were measured by multiplex suspension bead immunoassay. The impact of the EnSC secretome on human blastocyst development was scored morphologically; and embryo secretions in response to EnSC cues analyzed by multiplex suspension bead immunoassay. MAIN RESULTS AND THE ROLE OF CHANCE Clonogenicity and induction of decidual marker genes were comparable between EnSC cultures from pregnant and non-pregnant group groups (P > 0.05). Analysis of 23 secreted factors revealed that successful implantation was associated with co-ordinated secretome changes in decidualizing EnSCs, which were most pronounced on Day 2 of differentiation: 17 differentially secreted proteins on Day 2 of decidualization relative to undifferentiated (Day 0) EnSCs (P 0.05)

Il Modello ACASA per la stima degli scambi di carbonio negli ecosistemi mediterranei

L’attività di ricerca finalizzata allo sviluppo e alla validazione di modellistica avanzata per la contabilizzazione del bilancio del carbonio nei sistemi agrari e forestali nasce da una intensa collaborazione con l’Università della California. In particolare è in fase di studio il modello ACASA (Advanced Canopy-Atmosphere-Soil Algorithm), che è attualmente uno dei modelli del tipo soil-vegetation-atmosphere transfer (SVAT) più sofisticati. ACASA contiene equazioni differenziali di terzo ordine per simulare i flussi di energia e materia nella canopy (10 strati atmosferici all’interno e 10 al di sopra), mentre il suolo è suddiviso in 15 strati. Una combinazione delle equazioni di Ball-Berry e Farquhar è utilizzata per stimare il flusso di CO2. Il modello considera gli effetti dello stress idrico sulla traspirazione e sull’assimilazione della vegetazione

Advanced-Canopy-Atmosphere-Soil Algorithm (ACASA model) for estimating mass and energy fluxes

There is a recognized need to improve land surface models that simulate mass and energy fluxes between terrestrial ecosystems and atmosphere. In particular, long-term land planning strategies at local and regional scales require better understanding of agricultural ecosystem capacity to exchange CO2 and water. One of the more elaborate models for flux modelling is the Advanced Canopy-Atmosphere-Soil Algorithm (ACASA) model (Pyles et al., 2000), which provides micro-scale and regional-scale fluxes. The ACASA model allows for characterization of energy and carbon fluxes. It is a higher-order closure model used to estimate fluxes and profiles of heat, water vapor, carbon and momentum within and above canopy using third-order closure equations. It also estimates turbulent profiles of velocity, temperature, humidity within and above canopy. The ACASA model estimates CO2 fluxes using a combination of Ball-Berry and Farquhar equations. In addition, the effects of water stress on stomata, transpiration and CO2 assimilation are considered. The model was mainly used over dense canopies (Pyles et al. 2000, 2003) in the past, so the aim of this work was to test the ACASA model over a sparse canopy for estimating mass and energy fluxes, comparing model output with field measurements taken over a vineyard located in Montalcino, Tuscany, Italy

Impact of water filling on terminal ileum intubation with a distal-tip mucosal exposure device

Background and Aims Endocuff improves detection at colonoscopy but seems to impede terminal ileal (TI) intubation. We assessed the impact of Endocuff Vision (EV) on TI intubation using adult or pediatric colonoscopes and evaluated whether filling the cecum with gas versus water affected the impact of EV on TI intubation. Methods Using a prospectively recorded quality control database, we explored the impact of EV on TI intubation in ≤1 minute. We used adult and pediatric colonoscopes and tested the effect of filling the cecum with gas versus water. If the initial attempt failed, then the alternative (water vs gas) was tried as a rescue method. Results TI intubation in ≤1 minute occurred in 91% of colonoscopies without EV versus 65% with EV, but the use of the pediatric colonoscope with EV had a higher success rate for TI intubation in ≤1 minute compared with the adult colonoscope with EV (73% vs 57%, P = .043). TI intubation in ≤1 minute was more successful with EV when the cecum was filled with water rather than gas (74% vs 56%, P = .019), but the benefit of water filling was limited to the adult colonoscope with EV. When EV was in place, water filling was more successful as a rescue method of TI intubation (58% vs 21%, P = .011). Conclusions EV adversely affects TI intubation, particularly for adult colonoscopes. Water filling of the cecum mitigates the impact of EV on TI intubation with adult colonoscopes

Comprehensive Overview of Bottom-up Proteomics using Mass Spectrometry

Proteomics is the large scale study of protein structure and function from biological systems through protein identification and quantification. "Shotgun proteomics" or "bottom-up proteomics" is the prevailing strategy, in which proteins are hydrolyzed into peptides that are analyzed by mass spectrometry. Proteomics studies can be applied to diverse studies ranging from simple protein identification to studies of proteoforms, protein-protein interactions, protein structural alterations, absolute and relative protein quantification, post-translational modifications, and protein stability. To enable this range of different experiments, there are diverse strategies for proteome analysis. The nuances of how proteomic workflows differ may be challenging to understand for new practitioners. Here, we provide a comprehensive overview of different proteomics methods to aid the novice and experienced researcher. We cover from biochemistry basics and protein extraction to biological interpretation and orthogonal validation. We expect this work to serve as a basic resource for new practitioners in the field of shotgun or bottom-up proteomics

The “Flexi-Chamber”: A Novel Cost-Effective In Situ Respirometry Chamber for Coral Physiological Measurements

Coral reefs are threatened worldwide, with environmental stressors increasingly affecting the ability of reef-building corals to sustain growth from calcification (G), photosynthesis (P) and respiration (R). These processes support the foundation of coral reefs by directly influencing biogeochemical nutrient cycles and complex ecological interactions and therefore represent key knowledge required for effective reef management. However, metabolic rates are not trivial to quantify and typically rely on the use of cumbersome in situ respirometry chambers and/or the need to remove material and examine ex situ, thereby fundamentally limiting the scale, resolution and possibly the accuracy of the rate data. Here we describe a novel low-cost in situ respirometry bag that mitigates many constraints of traditional glass and plexi-glass incubation chambers. We subsequently demonstrate the effectiveness of our novel "Flexi-Chamber" approach via two case studies: 1) the Flexi-Chamber provides values of P, R and G for the reef-building coral Siderastrea cf. stellata collected from reefs close to Salvador, Brazil, which were statistically similar to values collected from a traditional glass respirometry vessel; and 2) wide-scale application of obtaining P, R and G rates for different species across different habitats to obtain inter- and intra-species differences. Our novel cost-effective design allows us to increase sampling scale of metabolic rate measurements in situ without the need for destructive sampling and thus significantly expands on existing research potential, not only for corals as we have demonstrated here, but also other important benthic groups

Surgical treatments compared with early structured physiotherapy in secondary care for adults with primary frozen shoulder: the UK FROST three-arm RCT

Background Frozen shoulder causes pain and stiffness. It affects around 10% of people in their fifties and is slightly more common in women. Costly and invasive surgical interventions are used, without high-quality evidence that these are effective. Objectives To compare the clinical effectiveness and cost-effectiveness of three treatments in secondary care for adults with frozen shoulder; to qualitatively explore the acceptability of these treatments to patients and health-care professionals; and to update a systematic review to explore the trial findings in the context of existing evidence for the three treatments. Design This was a pragmatic, parallel-group, multicentre, open-label, three-arm, randomised superiority trial with unequal allocation (2 : 2 : 1). An economic evaluation and a nested qualitative study were also carried out. Setting The orthopaedic departments of 35 hospitals across the UK were recruited from April 2015, with final follow-up in December 2018. Participants Participants were adults (aged ≥ 18 years) with unilateral frozen shoulder, characterised by restriction of passive external rotation in the affected shoulder to < 50% of the opposite shoulder, and with plain radiographs excluding other pathology. Interventions The inventions were early structured physiotherapy with a steroid injection, manipulation under anaesthesia with a steroid injection and arthroscopic capsular release followed by manipulation. Both of the surgical interventions were followed with post-procedural physiotherapy. Main outcome measures The primary outcome and end point was the Oxford Shoulder Score at 12 months post randomisation. A difference of 5 points between early structured physiotherapy and manipulation under anaesthesia or arthroscopic capsular release or of 4 points between manipulation under anaesthesia and arthroscopic capsular release was judged clinically important. Results The mean age of the 503 participants was 54 years; 319 were female (63%) and 150 had diabetes (30%). The primary analyses comprised 473 participants (94%). At the primary end point of 12 months, participants randomised to arthroscopic capsular release had, on average, a statistically significantly higher (better) Oxford Shoulder Score than those randomised to manipulation under anaesthesia (2.01 points, 95% confidence interval 0.10 to 3.91 points; p = 0.04) or early structured physiotherapy (3.06 points, 95% confidence interval 0.71 to 5.41 points; p = 0.01). Manipulation under anaesthesia did not result in statistically significantly better Oxford Shoulder Score than early structured physiotherapy (1.05 points, 95% confidence interval –1.28 to 3.39 points; p = 0.38). No differences were deemed of clinical importance. Serious adverse events were rare but occurred in participants randomised to surgery (arthroscopic capsular release, n = 8; manipulation under anaesthesia, n = 2). There was, however, one serious adverse event in a participant who received non-trial physiotherapy. The base-case economic analysis showed that manipulation under anaesthesia was more expensive than early structured physiotherapy, with slightly better utilities. The incremental cost-effectiveness ratio for manipulation under anaesthesia was £6984 per additional quality-adjusted life-year, and this intervention was probably 86% cost-effective at the threshold of £20,000 per quality-adjusted life-year. Arthroscopic capsular release was more costly than early structured physiotherapy and manipulation under anaesthesia, with no statistically significant benefit in utilities. Participants in the qualitative study wanted early medical help and a quicker pathway to resolve their shoulder problem. Nine studies were identified from the updated systematic review, including UK FROST, of which only two could be pooled, and found that arthroscopic capsular release was more effective than physiotherapy in the long-term shoulder functioning of patients, but not to the clinically important magnitude used in UK FROST. Limitations Implementing physiotherapy to the trial standard in clinical practice might prove challenging but could avoid theatre use and post-procedural physiotherapy. There are potential confounding effects of waiting times in the trial. Conclusions None of the three interventions was clearly superior. Early structured physiotherapy with a steroid injection is an accessible and low-cost option. Manipulation under anaesthesia is the most cost-effective option. Arthroscopic capsular release carries higher risks and higher costs. Future work Evaluation in a randomised controlled trial is recommended to address the increasing popularity of hydrodilatation despite the paucity of high-quality evidence. Trial registration Current Controlled Trials ISRCTN48804508. Funding This project was funded by the National Institute for Health Research (NIHR) Health Technology Assessment programme and will be published in full in Health Technology Assessment; Vol. 24, No. 71. See the NIHR Journals Library website for further project information