An analysis of expression patterns of genes encoding proteins with catalytic activities

Abstract Background In situ hybridization (ISH) is a powerful method for visualizing gene expression patterns at the organismal level with cellular resolution. When automated, it is capable of determining the expression of a large number of genes. Results The expression patterns of 662 genes that encode enzymes were determined by ISH in the mid-gestation mouse embryo, a stage that models the complexity of the adult organism. Forty-five percent of transcripts encoding metabolic enzymes (n = 297) showed a regional expression pattern. A similar percentage was found for the 190 kinases that were also analyzed. Many mRNAs encoding glycolytic and TCA cycle enzymes exhibited a characteristic expression pattern. The annotated expression patterns were deposited on the Genepaint database and are retrievable by user-defined queries including gene name and sites of expression. Conclusion The 662 expression patterns discussed here comprised gene products with activities associated with catalysis. Preliminary analysis of these data revealed that a significant number of genes encoding housekeeping functions such as biosynthesis and catabolism were expressed regionally, so they could be used as tissue-specific gene markers. We found no difference in tissue specificity between mRNAs encoding housekeeping functions and those encoding components of signal transduction pathways, as exemplified by the kinases.</p

An analysis of expression patterns of genes encoding proteins with catalytic activities-0

<p><b>Copyright information:</b></p><p>Taken from "An analysis of expression patterns of genes encoding proteins with catalytic activities"</p><p>http://www.biomedcentral.com/1471-2164/8/232</p><p>BMC Genomics 2007;8():232-232.</p><p>Published online 12 Jul 2007</p><p>PMCID:PMC1976134.</p><p></p> choroid plexi (cp, A) and gonads (g, B). () (MH1813, 4A) is exclusively expressed in cartilage (ca). and . ) (MH1633, 1A, 5A) is a suitable marker for lens (le, D) and choroid plexi (cp, E). and . () (MH1439, 4C, 4B) marks the ventricular zones of the nervous system (vz, F), including that of the spinal cord (sp, G), and liver (not shown). . The gene () (MH1654, 5B) marks liver (li), pancreas (pa) and stomach (so). and . Expression of () (MH1744, 3D) is seen in adrenal gland (a, I), lung mesenchyme (lu, I) and nasal epithelium (n, J). and . () (MH1625, 3A) expression is restricted to the lung (lu, K) and the adrenal gland (a, L). and . () (MH1608, 4C) is exclusive to the ventromedial nucleus of the hypothalamus (vt), preoptic area, anterior pituitary (pi, M) and pancreas (pa, N), . Expression of () (MH1292, 3C, 1A) in choroids plexi (cp, O and P) and retina (re, Q)

An analysis of expression patterns of genes encoding proteins with catalytic activities-3

<p><b>Copyright information:</b></p><p>Taken from "An analysis of expression patterns of genes encoding proteins with catalytic activities"</p><p>http://www.biomedcentral.com/1471-2164/8/232</p><p>BMC Genomics 2007;8():232-232.</p><p>Published online 12 Jul 2007</p><p>PMCID:PMC1976134.</p><p></p> choroid plexi (cp, A) and gonads (g, B). () (MH1813, 4A) is exclusively expressed in cartilage (ca). and . ) (MH1633, 1A, 5A) is a suitable marker for lens (le, D) and choroid plexi (cp, E). and . () (MH1439, 4C, 4B) marks the ventricular zones of the nervous system (vz, F), including that of the spinal cord (sp, G), and liver (not shown). . The gene () (MH1654, 5B) marks liver (li), pancreas (pa) and stomach (so). and . Expression of () (MH1744, 3D) is seen in adrenal gland (a, I), lung mesenchyme (lu, I) and nasal epithelium (n, J). and . () (MH1625, 3A) expression is restricted to the lung (lu, K) and the adrenal gland (a, L). and . () (MH1608, 4C) is exclusive to the ventromedial nucleus of the hypothalamus (vt), preoptic area, anterior pituitary (pi, M) and pancreas (pa, N), . Expression of () (MH1292, 3C, 1A) in choroids plexi (cp, O and P) and retina (re, Q)

An analysis of expression patterns of genes encoding proteins with catalytic activities-2

<p><b>Copyright information:</b></p><p>Taken from "An analysis of expression patterns of genes encoding proteins with catalytic activities"</p><p>http://www.biomedcentral.com/1471-2164/8/232</p><p>BMC Genomics 2007;8():232-232.</p><p>Published online 12 Jul 2007</p><p>PMCID:PMC1976134.</p><p></p>ellow, medium; and blank, weak expression. In cases where transcripts could not be detected, boxes are shown in blue. The Genepaint set IDs point to the data sets from which quantitative assessments were made. Genes showing a SEMP are in bold