Reaction norm plots of hatching time, larvae length, and yolk sac volume.

<p>In the upper panel, each line corresponds to sire (N = 20) means across all females. Lines in the lower panel represent means (±SD) per within-population (black) or between-population cross (light grey). PF: <i>P. fluorescens</i>.</p

Breeding design.

<p>Females are indicated by letters, males by numbers, and crosses by black squares.</p

Likelihood ratio tests on mixed model logistic regression on plastic traits.

<p>Reference models are indicated in bold. To test the effect of treatment, the reference model was compared to a model lacking treatment. For the other effects, the reference model was compared to a model incorporating the effect of interest. t: treatment; p: population cross type (within vs. between); s: sire; d: dam</p

Likelihood ratio tests on mixed model logistic regressions assessing the effects of genetic distance (F_ST) and breeder relatedness (<i>W</i>) on hatching time and larval length in the two treatments.

<p>F_ST (as a linear or quadratic predictor) and <i>W</i> (linear predictor) were entered as fixed effects, while sire, dam, and sire x dam interaction effects were entered as random effects. Models incorporating F_ST or <i>W</i> were then compared to the reference models (indicated in bold). s: sire; d: dam.</p

Mean time to hatching (A), larvae length, and yolk sac volume (B) (±SE) by treatment.

<p>In panel B, dark grey corresponds to larval length, and light grey to yolk sac volume.</p

Locations of the five study populations in the Aare River catchment.

<p>Map adapted from Stelkens et al. 2012.</p

MolEcol Dead and Hatch data

Embryo survival and day of hatching. Each line represents one singly reared embryo exposed to one of the treatments. "Female" and "Male" give the embryo's mother and father, respectively

Functional Ecology data file 2

Hatchling lenght and yolk sac dimension

Functional Ecology data file 1

Embryo mortality and hatching tim

The eIF2α kinase PKR negatively regulates invasion and HRI positively regulates intracellular proliferation of <i>Chlamydia</i>.

<p>(<b>A</b>) <i>Pkr</i> +/+ and -/- (left) and <i>Hri</i> +/+ and -/- (right) MEFs were infected with <i>C. trachomatis</i> L2 for 24 hr and then stained for <i>C. trachomatis</i>-containing inclusion bodies. The total number of inclusion forming units is plotted. (<b>B</b>) Infected <i>Hri</i> +/+ and -/- MEFs are shown with arrows denoting underdeveloped <i>C</i><i>. tranchomatis</i>-containing inclusions in the <i>Hri</i> -/- cells. (<b>C</b>) Cells were infected as described and were then harvested and the resulting lysates used to infect cultured HeLa cells to determine their titer (plotted as ‘progeny counts’). (<b>D</b>) Unstimulated peritoneal macrophages were isolated from <i>Hri</i> +/+ and -/- mice and infected as described. <i>P</i> values calculated using student <i>t</i> test.</p