Contención química de un puma (puma concolor) mediante ketamina-medetomidina, revertida con atipamezol, en Tamaulipas, México

Chemical restraint of a free-ranging male cougar (Puma concolor) was carried out with Ketamine (90 mg) and Medetomidine (1.2 mg) by an airgun. During the chemical restraint, was necessary to apply additional 75 mg of ketamine. The procedure was reverted with atipamezol. During chemical restraint the animal had excessive salivation and vomit

The mitochondrial activity of leukocytes from Artibeus jamaicensis bats remains unaltered after several weeks of flying restriction

Bats are the only flying mammals known. They have longer lifespan than other mammals of similar size and weight and can resist high loads of many pathogens, mostly viruses, with no signs of disease. These distinctive characteristics have been attributed to their metabolic rate that is thought to be the result of their flying lifestyle. Compared with non-flying mammals, bats have lower production of reactive oxygen species (ROS), and high levels of antioxidant enzymes such as superoxide dismutase. This anti-oxidative vs. oxidative profile may help to explain bat's longer than expected lifespans. The aim of this study was to assess the effect that a significant reduction in flying has on bats leukocytes mitochondrial activity. This was assessed using samples of lymphoid and myeloid cells from peripheral blood from Artibeus jamaicensis bats shortly after capture and up to six weeks after flying deprivation. Mitochondrial membrane potential (Δψm), mitochondrial calcium (mCa2+), and mitochondrial ROS (mROS) were used as key indicators of mitochondrial activity, while total ROS and glucose uptake were used as additional indicators of cell metabolism. Results showed that total ROS and glucose uptake were statistically significantly lower at six weeks of flying deprivation (p 0.05). These results suggest that bat mitochondria are stable to sudden changes in physical activity, at least up to six weeks of flying deprivation. However, decrease in total ROS and glucose uptake in myeloid cells after six weeks of captivity suggest a compensatory mechanism due to the lack of the highly metabolic demands associated with flying

Clarifying the Cryptic Host Specificity of <i>Blastocystis</i> spp. Isolates from <i>Alouatta palliata</i> and <i>A</i>. <i>pigra</i> Howler Monkeys

<div><p>Although the presence of cryptic host specificity has been documented in <i>Blastocystis</i>, differences in infection rates and high genetic polymorphism within and between populations of some subtypes (ST) have impeded the clarification of the generalist or specialist specificity of this parasite. We assessed the genetic variability and host specificity of <i>Blastocystis</i> spp. in wild howler monkeys from two rainforest areas in the southeastern region of Mexico. Fecal samples of 225 <i>Alouatta palliata</i> (59) and <i>A</i>. <i>pigra</i> (166) monkeys, belonging to 16 sylvatic sites, were analyzed for infection with <i>Blastocystis</i> ST using a region of the small subunit rDNA (SSUrDNA) gene as a marker. Phylogenetic and genetic diversity analyses were performed according to the geographic areas where the monkeys were found. <i>Blastocystis</i> ST2 was the most abundant (91.9%), followed by ST1 and ST8 with 4.6% and 3.5%, respectively; no association between <i>Blastocystis</i> ST and <i>Alouatta</i> species was observed. SSUrDNA sequences in GenBank from human and non-human primates (NHP) were used as ST references and included in population analyses. The haplotype network trees exhibited different distributions: ST1 showed a generalist profile since several haplotypes from different animals were homogeneously distributed with few mutational changes. For ST2, a major dispersion center grouped the Mexican samples, and high mutational differences were observed between NHP. Furthermore, nucleotide and haplotype diversity values, as well as migration and genetic differentiation indexes, showed contrasting values for ST1 and ST2. These data suggest that ST1 populations are only minimally differentiated, while ST2 populations in humans are highly differentiated from those of NHP. The host generalist and specialist specificities exhibited by ST1 and ST2 <i>Blastocystis</i> populations indicate distinct adaptation processes. Because ST1 exhibits a generalist profile, this haplotype can be considered a metapopulation; in contrast, ST2 exists as a set of local populations with preferences for either humans or NHP.</p></div

Schematic representation of interactions among population indexes.

<p>The gene flow (Nm), genetic differentiation index (F_ST), and Tajima’s D values of <i>Blastocystis</i> ST by SSUrDNA analysis, according to different sampling sites; only those sites in which there were enough infected howlers to obtain the indexes are shown. The number together the sampling size circle, mean the Tajima’s D value. * <i>p</i><0.01</p

Haplotype networks for <i>Blastocystis</i>.

<p>Haplotype network trees using SSUrDNA sequences from different countries and hosts for ST1 (a) and ST2 (b). Numbers in branches refer to mutational changes; sizes of circles and colors are proportional to haplotype frequencies. For those animal haplotypes, an image and Roman reference numbers were included, while for human haplotypes, asterisks were added.</p

Infection rates of <i>Blastocystis</i> subtype (ST) for howler monkey populations.

<p>Infection rates of <i>Blastocystis</i> subtype (ST) for howler monkey populations.</p

Outcomes in Newly Diagnosed Atrial Fibrillation and History of Acute Coronary Syndromes: Insights from GARFIELD-AF

BACKGROUND: Many patients with atrial fibrillation have concomitant coronary artery disease with or without acute coronary syndromes and are in need of additional antithrombotic therapy. There are few data on the long-term clinical outcome of atrial fibrillation patients with a history of acute coronary syndrome. This is a 2-year study of atrial fibrillation patients with or without a history of acute coronary syndromes