Инсулин-позитивные клетки печени и экзокринной части поджелудочной железы у животных с экспериментальным сахарным диабетом

Aim. To compare the number of insulin+ cells in the liver and exocrine part of the pancreas with the type of experimental diabetes, blood glycose and glycated hemoglobin (HbA1c) level and with the number of Pdx1+ cells. Materials and methods. The experiment was carried out on 25 male Wistar rats (weighting (303.0 ± 25.3) g) that were divided into 3 groups: The first group consisted of intact animals, the second had animals with experimental diabetes type 1, and the third with animals with experimental diabetes type 2. Biochemical, immunohistochemical, ELISA methods and statistical analysis were used. Results. Insulin+ and Pdx1+ cells of rats with experimental diabetes were found in the liver and exocrine part of pancreas. The highest number of insulin+ cells in the liver was detected in type 2 diabetes (T2D). A strong positive correlation between the number of insulin+ cells in the liver and level of glycosylated hemoglobin in theblood was revealed in both type 1 and type 2 diabetes. Conclusion. Insulin+ cells are detected in the liver and acinar part of pancreas of both intact rats and rats with experimental diabetes. Group with T2D is characterized by the highest number of insulin+ cells in the liver compared with type 1 diabetes (T1D). The localization of insulin+ cells in the liver changes depending on the type of diabetes. In T2D insulin+ cells are located in all parts of liver acini, meanwhile in animals with T1D such cells are mainly detected in the periportal area. The expression of Pdx1+ in acinar cells of pancreas and liver cells is likely a mechanism for their reprogramming into insulin+ cells in experimental diabetes mellitus. © 2020 Siberian State Medical University. All rights reserved.This work was financially supported by the Russian Science Foundation, grant No. 16-1500039-P and the budget project No. АААА-А18-118020590108-7 of the Institute of Immunology and Physiology, UB RAS

Preparation for the VIP-CRIT space experiment on the ISS : an analysis of MIR experiments and ground-based studies of heat transfer and phase separation in near-critical fluid

We present a review of recent activity of the VIP-CRIT team in analyzing the previous space experiments, developing analytical methods, and carrying out the laboratory and numerical simulation devoted to the study of the effects of microgravity environment on the heat transfer and phase separation in the supercritical and near-critical fluids...

Convection and heat transfer experiments in supercritical fluid under microgravity: from MIR to ISS

The results of two series of space experiments carried out onboard the MIR in 1999—2000 are presented. The experiments were undertaken to study the effect of microgravity environment as well as specially excited vibration on heat spread from a point source inside a cell filled with SF6 near its critical point. New space experiments are proposed to continue the series interrupted by the deorbiting of the MIR. These proposals are the central point of the CRIT project planned for the realization onboard the Russian Segment of the ISS