Influence of Insulin-like Growth Factor I (IGF-I) on the survival and the in vitro development of caprine preantral follicles

The aim of this study was to investigate the effects of the insulin-like growth factor -I (IGF-I) on survival, activation (transition from primordial to primary follicles) and growth of caprine preantral follicles cultured in vitro. Fragments of ovarian cortex were cultured for one and seven days in the absence or presence of IGF-I (0, 50 and 100ng/ml). The non-cultured and cultured tissues were processed and analyzed by histology and transmission electron microscopy. The culture for one day in a medium with 100ng/ml of IGF-I showed 86.7% of morphologically normal follicles. These results were similar (P>0.05) to the percentage of normal follicles found in the control (96.7%). It was also found that this medium increased the percentage of follicular activation (developing follicles) with one day of culture. The oocyte and follicular diameters remained similar to the control by culturing for one day in a medium containing 100ng/ml of IGF-I. The ultrastructural analysis did not confirm the integrity of the follicular fragments in a medium containing IGF-I (100ng/ml) after one and seven days of culture. In conclusion, this study demonstrated that the addition of 100 ng/ml of IGF-I in the culture medium enables the development of preantral follicles of goats with one day of culture. However, it is not sufficient to maintain the follicular integrity and the follicular survival rate after seven days of culture.O objetivo do presente estudo foi investigar os efeitos do fator de crescimento semelhante a insulina-I (IGF-I) na sobrevivência, ativação (transição de folículos primordiais para primários) e crescimento de folículos pré-antrais caprinos cultivados in vitro. Fragmentos de córtex ovariano foram cultivados por um e sete dias na ausência ou presença de IGF-I (0, 50 e 100ng/mL). Os tecidos não cultivados e cultivados foram processados e analisados por histologia e microscopia eletrônica de transmissão. O cultivo por um dia em meio com 100ng/mL de IGF-I apresentou 86,7% de folículos morfologicamente normais. Estes resultados foram semelhantes (P>0,05) ao percentual de folículos normais encontrados no controle (96,7%). Verificou-se ainda que este meio aumentou o percentual de ativação folicular (folículos em desenvolvimento) com um dia de cultivo. Os diâmetros ovocitário e folicular mantiveram-se semelhantes ao controle ao cultivar por um dia em meio contendo 100ng/mL de IGF-I. As análises ultraestruturais não confirmaram a integridade folicular dos fragmentos em meio contendo IGF-I (100 ng/mL) após um e sete dias de cultivo. Em conclusão, esse estudo demonstrou que a adição de 100 ng/mL de IGF-I no meio de cultivo ativa o desenvolvimento de folículos pré-antrais de caprinos com um dia de cultivo. Entretanto, não é suficiente para manter a integridade folicular e a taxa de sobrevivência folicular após sete dias de cultivo

Hiperplasia endometrial cística e hidrossalpinge associada a cisto folicular em bovinos

Relata-se neste trabalho a ocorrência de cistos ovarianos, endometriais e hidrossalpinge esquerda em uma fêmea bovina. O diagnóstico de tais patologias foi realizado por meio da palpação e histopatológico post-mortem. No ovário direito foi detectado extensas áreas flutuantes, que correspondiam a três grandes áreas anecóicas de 30 mm de diâmetro ao exame ultrassonográfico. Este achado é característico de estruturas císticas no ovário, e foram classificadas como cistos foliculares, devido à ausência de luteinização da parede folicular. Foi realizada a dosagem da concentração de estrógeno (17ß estradiol) e progesterona no líquido folicular, observando-se 44,8 ng/mL e 15,7 ng/mL respectivamente, e no soro sanguíneo 35,8 pg/mL e 0,2 ng/ mL, respectivamente. Na avaliação ultrassonográfica do útero, foi observado presença de áreas ane- cóicas bem delimitadas no endométrio, indicando que havia formações císticas, além de pouca quantidade de líquido no lúmen uterino. Também por ultrassonografia, foi observado um espessamento na parede e acúmulo de líquido na tuba uterina esquerda, diagnosticada como hidrossalpinge. Histologicamente, os cistos foram classificados em cistos foliculares do tipo I e as alterações no útero foram compatíveis com hiperplasia cística do endométrio. Neste caso, a utilização das análises anatomo e histopatológicas foram úteis para a confirmação dos achados. No entanto, o estudo demonstra que as patologias descritas podem ser diagnosticadas baseando-se apenas nos exames de palpação e ultrassonografia transretal.We report herein the occurrence of ovarian cysts, endometrial cysts and left hydrosalpinx in a bovine female. The diagnosis of such conditions was performed by rectal palpation and ultrasonography of the reproductive organs in addition to the correlation with hormone levels (estrogen and progesterone) in blood serum. Additionally, anatomical and histopathological examinations were performed post-mortem. In the right ovary extensive floating areas, corresponding to three major areas of anechoic 30 mm in diameter was detected by ultrasonography. This finding is characteristic of ovarian cystic structures, and was classified as follicular cysts, due to the absence of follicular luteinization wall. The determination of the concentration of estrogen (estradiol 17ß) and progesterone in follicular fluid was performed by observing 44.8 ng/mL and 15.7 ng/mL, respectively , and in the serum 35.8 pg/mL and 0.2 ng/mL, respectively. In ultrassonographic assessment of the uterus was observed the presence of well-defined endometrium anechoic areas, indicating cystic formations, plus a small amount of liquid in the uterine lumen. A thickening of the wall and accumulation of fluid in the left uterine tube was also observed by ultrasonography and diagnosed as hydrosalpinx. Histologically, the cysts were classified as follicular cysts type I and changes in the uterus were consistent with cystic endometrial hyperplasia. In this case, the use of anatomical and hitopatological analyzes were useful to confirm the findings. However, the study shows that the conditions described can be diagnosed based solely on palpation and transrectal ultrasonography

Cystic endometrial hyperplasia and hydrosalpinx associated with follicular cyst in cattle

ABSTRACT. Pereira J.V.T.N., Pinho R.O., Meneses R.M., Mendes V.R.A., Pereira E.C.M., Pereira C.E.R. & Guimarães J.D. [Cystic endometrial hyperplasia and hydrosalpinx associated with follicular cyst in cattle.] Hiperplasia endometrial cística e hidrossalpinge associada a cisto folicular em bovinos. Revista Brasileira de Medicina Veterinária, 37(1):1-6, 2015. Departamento de Zootecnia, Universidade Federal de Viçosa, Av. Peter H Rolfs, s/n, Viçosa, MG 36570-000, Brasil. E-mail: [email protected] We report herein the occurrence of ovarian cysts, endometrial cysts and left hydrosalpinx in a bovine female. The diagnosis of such conditions was performed by rectal palpation and ultrasonography of the reproductive organs in addition to the correlation with hormone levels (estrogen and progesterone) in blood serum. Additionally, anatomical and histopathological examinations were performed post-mortem. In the right ovary extensive floating areas, corresponding to three major areas of anechoic 30 mm in diameter was detected by ultrasonography. This finding is characteristic of ovarian cystic structures, and was classified as follicular cysts, due to the absence of follicular luteinization wall. The determination of the concentration of estrogen (estradiol 17ß) and progesterone in follicular fluid was performed by observing 44.8 ng/mL and 15.7 ng/mL, respectively , and in the serum 35.8 pg/mL and 0.2 ng/mL, respectively. In ultrassonographic assessment of the uterus was observed the presence of well-defined endometrium anechoic areas, indicating cystic formations, plus a small amount of liquid in the uterine lumen. A thickening of the wall and accumulation of fluid in the left uterine tube was also observed by ultrasonography and diagnosed as hydrosalpinx. Histologically, the cysts were classified as follicular cysts type I and changes in the uterus were consistent with cystic endometrial hyperplasia. In this case, the use of anatomical and hitopatological analyzes were useful to confirm the findings. However, the study shows that the conditions described can be diagnosed based solely on palpation and transrectal ultrasonography