45 research outputs found
Healthy lifestyles of the university population
El estilo de vida se define como el conjunto de pautas y hábitos comportamentales cotidianos de una persona, que mantenidos en el tiempo pueden constituirse en dimensiones de riesgo o de seguridad dependiendo de su naturaleza. Los objetivos del presente trabajo han sido conocer los estilos de vida de los jóvenes universitarios en las siguientes dimensiones: alimentación, ejercicio físico, consumo de tabaco, alcohol y otras drogas, relaciones sexuales y seguridad vial. Para ello se ha realizado una revisión bibliográfica en las bases de datos electrónicas: PUBMED, SCIELO y CUIDEN entre los años 2002-2014; utilizando como palabras claves: estilos de vida, conductas saludables, dieta, ejercicio, drogas, estudiantes universitarios. De los artículos encontrados, destacar como dato más relevante, que los jóvenes universitarios poseen una alta presencia de creencias favorables sobre hábitos de vida saludables y a pesar de ello, no lo llevan a la práctica. Tras realizar una exhaustiva revisión podemos concluir que según los distintos autores, los jóvenes universitarios en general, no tienen buenos hábitos alimentarios, consumiendo dietas desequilibradas con alto contenido calórico. Además la práctica del ejercicio físico es nula, aun sabiendo que una buena alimentación y la realización de ejercicio tienen efectos beneficiosos sobre la salud. A esto hay que añadirle el consumo elevado de alcohol, tabaco y marihuana entre los jóvenes universitarios.The lifestyle is defined as the set of behavioral patterns
and daily habits of a person, which maintained over time
may become dimensions of risk or safety depending on
their nature.
The aim of this study was to know the lifestyles of university
students in the following dimensions: diet, exercise,
consumption of tobacco, alcohol and other drugs, sex
and road safety.
We made a literature review in electronic databases:
PubMed, SCIELO and CUIDEN, between 2002-2014;
using as keywords habits, lifestyle, health behaviors,
young adult and university students.
From articles found, stand out as most relevant data
that university students have a high presence of favorable
beliefs about healthy lifestyles and nevertheless not
put into practice.
We could conclude that according to different authors,
university students in general have not a good eating
habits, eating unbalanced diets high in calories. Besides
the physical exercise is null, knowing that a good diet and
doing exercise have beneficial effects on health. To this
must be added the high consumption of alcohol, tobacco
and marijuana among university students
Therapeutic doses of nonsteroidal anti-inflammatory drugs inhibit osteosarcoma MG-63 osteoblast-like celss maturation, viability, and biomineralization potential
Nonsteroidal anti-inflammatory drugs (NSAIDs) are frequently used to reduce pain and inflammation. However, their effect on bone metabolisms is not well known, and results in the literature are contradictory. The present study focusses on the effect of dexketoprofen, ketorolac, metamizole, and acetylsalicylic acid, at therapeutic doses, on different biochemical and phenotypic pathways in human osteoblast-like cells. Osteoblasts (MG-63 cell line) were incubated in culture medium with 1–10 M of dexketoprofen, ketorolac, metamizole, and acetylsalicylic acid. Flow cytometry was used to study antigenic profile and phagocytic activity. The osteoblastic differentiation was evaluated by mineralization and synthesis of collagen fibers by microscopy and alkaline phosphatase activity (ALP) by spectrophotometric assay. Short-term treatment with therapeutic doses of NSAIDs modulated differentiation, antigenic profile, and phagocyte activity of osteoblast-like cells. The treatment reduced ALP synthesis and matrix mineralization. However, nonsignificant differences were observed on collagen syntheses after treatments. The percentage of CD54 expression was increased with all treatments. CD80, CD86, and HLA-DR showed a decreased expression, which depended on NSAID and the dose applied. The treatments also decreased phagocyte activity in this cellular population. The results of this paper provide evidences that NSAIDs inhibit the osteoblast differentiation process thus reducing their ability to produce new bone mineralized extracellular matrix.This study was supported by the BIO277 research group (Junta de Andalucía), by the Department of Nursing, Faculty of Health Sciences, University of Granada and by the research group Brighton Studies in Tissue-mimicry and Aided Regeneration (BrightSTAR), School of Pharmacy & Biomolecular Sciences, University of Brighton
Analysis of the Lifestyle of Spanish Undergraduate Nursing Students and Comparison with Students of Other Degrees
Background: Nursing students are exposed to concepts of healthy lifestyles while they are
attending university. Objective: The aim of this study was to analyze whether nursing students have
a healthier lifestyle than non-nursing students and to determine whether their behaviour is consistent
with their beliefs. Methods: A cross-sectional study, with 293 university students was performed
by using a validated questionnaire to measure beliefs and behaviour regarding health. Results:
The lifestyle pattern of the nursing students evaluated was characterised by a high percentage of
nurses with low levels of physical activity, poor balanced diet and smoking habits. The comparative
analysis showed no significant differences between nursing students and students from other degrees.
Conclusions: Students have a positive attitude and knowledge about healthy lifestyle, but do not
transfer it to their own lives. Nurses’ lifestyle can unintentionally affect the behaviour of other people
through their own behaviour and beliefs because they serve as a model for a healthy lifestyle. These
findings support that nurse educators have an active role as promoter of health by using lessons to
modify the behaviour of their students
Role of Vitamin D in the Metabolic Syndrome
This study was supported by research group BIO277 (Junta de Andalucía) and
the Department of Nursing (University of Granada).The prevalence of hypovitaminosis D has risen in developed countries over the past few years in association with lifestyle changes and an increase in unhealthy habits. Vitamin D deficiency has been implicated in various diseases, including metabolic syndrome (MetS), which is clinically defined by a set of metabolic and vascular disorders. The objective of this study was to review scientific evidence on the relationship between MetS and vitamin D deficiency to support the development of prevention strategies and health education programs. An inverse relationship has been reported between plasma vitamin D concentrations and the features that define MetS, i.e., elevated serum concentrations of glucose, total cholesterol, low-density lipoproteins, triglycerides, glycosylated hemoglobin, and a high body mass index. Numerous studies have described the benefits of vitamin D supplementation to improve outcomes in individuals with MetS. Interventions to maintain optimal vitamin D concentrations are proposed as a preventive strategy against MetS.Junta de AndaluciaDepartment of Nursing (University of Granada
Biological properties and therapeutic applications of garlic and its components
This study was supported by research group BIO277 (Junta de Andalucia) and Department of Nursing (University of Granada).Garlic is one of the most widely employed condiments in cooking. It has also been used since ancient
times in traditional plant-based medicine, largely based on its organosulfur compounds. The objective of
this study was to provide updated information on the biological and therapeutic garlic properties. Garlic
has been found to possess important biological properties with high therapeutic potential, which is
influenced by the mode of its utilization, preparation, and extraction. It has been attributed with antioxidant,
anti-inflammatory, and immunomodulatory capacities. Garlic, in particular its organosulfur compounds,
can maintain immune system homeostasis through positive effects on immune cells, especially
by regulating cytokine proliferation and expression. This may underlie their usefulness in the treatment of
infectious and tumor processes. These compounds can also offer vascular benefits by regulating lipid
metabolism or by exerting antihypertensive and antiaggregant effects. However, further clinical trials are
warranted to confirm the therapeutic potential of garlic and its derivatives.Department of Nursing (University of Granada)Junta de Andaluci
Potential Effects of Phenolic Compounds That Can Be Found in Olive Oil on Wound Healing
This study was supported by research group BIO277 (Junta de Andalucia) and the Department of Nursing of the University of Granada. We would also like to thank Concepcion Ruiz for the considerations and retouches made to this paper.The treatment of tissue damage produced by physical, chemical, or mechanical agents
involves considerable direct and indirect costs to health care systems. Wound healing involves a series
of molecular and cellular events aimed at repairing the defect in tissue integrity. These events can be
favored by various natural agents, including the polyphenols in extra virgin olive oil (EVOO). The
objective of this study was to review data on the potential effects of different phenolic compounds
that can also be found in EVOO on wound healing and closure. Results of in vitro and animal
studies demonstrate that polyphenols from different plant species, also present in EVOO, participate
in different aspects of wound healing, accelerating this process through their anti-inflammatory,
antioxidant, and antimicrobial properties and their stimulation of angiogenic activities required for
granulation tissue formation and wound re-epithelialization. These results indicate the potential
usefulness of EVOO phenolic compounds for wound treatment, either alone or in combination with
other therapies. Human studies are warranted to verify this proposition.Junta de Andalucia BIO277Department of Nursing of the University of Granad
Biomimetic Collagen Membranes as Drug Carriers of Geranylgeraniol to Counteract the Effect of Zoledronate
To counteract the effect of zoledronate and decrease the risk of osteonecrosis of the jaw
(BRONJ) development in patients undergoing guided bone regeneration surgery, the use of geranylgeraniol
(GGOH) has been proposed. Collagen membranes may act as biomimetical drug carriers.
The objective of this study was to determine the capacity of collagen-based membranes doped with
GGOH to revert the negative impact of zoledronate on the growth and differentiation of human
osteoblasts. MG-63 cells were cultured on collagen membranes. Two groups were established:
(1) undoped membranes and (2) membranes doped with geranylgeraniol. Osteoblasts were cultured
with or without zoledronate (50 μM). Cell proliferation was evaluated at 48 h using the MTT colorimetric
method. Differentiation was tested by staining mineralization nodules with alizarin red and
by gene expression analysis of bone morphogenetic proteins 2 and 7, alkaline phosphatase (ALP),
bone morphogenetic proteins 2 and 7 (BMP-2 and BMP-7), type I collagen (Col-I), osterix (OSX),
osteocalcin (OSC), osteoprotegerin (OPG), receptor for RANK (RANKL), runt-related transcription
factor 2 (Runx-2), TGF-β1 and TGF-β receptors (TGF-βR1, TGF-βR2, and TGF-βR3), and vascular
endothelial growth factor (VEGF) with real-time PCR. One-way ANOVA or Kruskal–Wallis and post
hoc Bonferroni tests were applied (p < 0.05). Scanning electron microscopy (SEM) observations were
also performed. Treatment of osteoblasts with 50 μM zoledronate produced a significant decrease in
cell proliferation, mineralization capacity, and gene expression of several differentiation markers if
compared to the control (p < 0.001). When osteoblasts were treated with zoledronate and cultured on
GGOH-doped membranes, these variables were, in general, similar to the control group (p > 0.05).
GGOH applied on collagen membranes is able to reverse the negative impact of zoledronate on the
proliferation, differentiation, and gene expression of different osteoblasts’ markers.Grant PID2020-114694RB-I00 funded by MCIN/AEI 10.13039/501100011033FPU of Ministry of Universities [grant FPU20/00450
Human Fibroblast Gene Expression Modulation Using 940 NM Diode Laser
Low-Level Laser Therapy is used as regenerative therapy in different clinical fields. This is due to its
photobiomodulation effect via cell signaling on different cell populations, Including fibroblasts, cells
involved in tissue regeneration and healing. The aim was to analyze the effect of 940 nm diode laser on
the gene expression of different markers involved in fibroblast growth, differentiation, and migration.
Real-time polymerase chain reaction (q-RT-PCR) was used to quantify the expression of fibroblast
growth factor (FGF), connective tissue growth factor (CTGF), vascular-endothelial growth factor
(VEGF), transforming growth factor β1 (TGF-β1), TGFβ-receptors (TGFβR1, TGFβR2, and TGFβR3),
discoidin-domain receptor-2 (DDR2), matrix metalloproteinase-2 (MMP2), α-actin, fibronectin, decorin,
and elastin on human fibroblast, treated with single dose (T1) or two doses (T2) of diode laser at 0.5
Watts and 4 J/cm2. A significant increase in the expression of FGF, TGF-β1, TGFβR1, TGFβR2, α-actin,
fibronectin, decorin, DDR2 and MMP2 was observed after both treatments. A decrease was observed
in expression of elastin (T1 and T2), and CTGF (T2). These changes underlie the biostimulatory effect
of laser on fibroblasts, which translates into an increase in short-term proliferation and in long-term
differentiation to myofibroblasts. These data support the therapeutic potential of diode laser for wound
repair
Effects of bisphenol F, bisphenol S, and bisphenol AF on cultured human osteoblasts
Bisphenol A (BPA) analogs, like BPA, could have adverse effects on human health including bone health. The aim was to
determine the effect of BPF, BPS and BPAF on the growth and differentiation of cultured human osteoblasts. Osteoblasts
primary culture from bone chips harvested during routine dental work and treated with BPF, BPS, or BPAF for 24 h at doses
of 10 –5 , 10 –6 , and 10 –7 M. Next, cell proliferation was studied, apoptosis induction, and alkaline phosphatase (ALP) activity.
In addition, mineralization was evaluated at 7, 14, and 21 days of cell culture in an osteogenic medium supplemented with BP
analog at the studied doses. BPS treatment inhibited proliferation in a dose-dependent manner at all three doses by inducing
apoptosis; BPF exerted a significant inhibitory effect on cell proliferation at the highest dose alone by an increase of apopto-
sis; while BPAF had no effect on proliferation or cell viability. Cell differentiation was adversely affected by treatment with
BPA analogs in a dose-dependent, observing a reduction in calcium nodule formation at 21 days. According to the results
obtained, these BPA analogs could potentially pose a threat to bone health, depending on their concentration in the organism.Funding for open access publishing: Universidad de Granada/
CBU
Bone Protective Effect of Extra-Virgin Olive Oil Phenolic Compounds by Modulating Osteoblast Gene Expression
The phenolic compounds of extra-virgin olive oil can act at various levels to protect
individuals against cardiovascular and neurodegenerative diseases, cancer, and osteoporosis, among
others. Polyphenols in extra-virgin olive oil can stimulate the proliferation of osteoblasts, modify
their antigen profile, and promote alkaline phosphatase synthesis. The objective of this work was to
determine the effect of different extra-virgin olive oil phenolic compounds on the gene expression
of osteoblast-related markers. The cells of the MG63 osteoblast line were cultured for 24 h with
10-6 M of the phenolic compounds ferulic acid, caffeic acid, coumaric acid, apigenin, or luteolin.
The expression of studied markers was quantified using quantitative real-time polymerase chain
reaction (q-RT-PCR). The expression by MG63 osteoblasts of growth and differentiation/maturation
markers was modified after 24 h of treatment with 10-6 M of the phenolic compounds under study,
most of which increased the gene expression of the transforming growth factor 1 (TGF- 1), TGF-
receptor 1,2 and 3 (TGF- R1, TGF- R2, TGF- R3), bone morphogenetic protein 2 and 7 (BMP2,
BMP7), run-related transcription factor 2 (RUNX-2), Alkaline phosphatase (ALP), Osteocalcin (OSC),
Osterix (OSX), Collagen type I (Col-I) and osteoprotegerin (OPN). The extra-virgin olive oil phenolic
compounds may have a beneficial effect on bone by modulating osteoblast physiology, which would
support their protective effect against bone pathologies.The work outlined in this article has been partially funded by the Spanish Ministry of Education under
FPU fellowship reference FPU15-0563