Pastor.i: a smartphone application to facilitate grazing management

Grazing in extensive beef farming systems is often manage in an empirical way based on past experience and on the visual appreciation of animal behavior and forage potential. Records of entrances and exits of the animals in the paddocks are rare. However, knowing the occupation period and the animal density, when coupled with biomass defines the grazing pressure. This knowledge is essential for planning and making informed decisions, that influence the profitability of the farm. Moreover, adequate grazing pressure is crucial for the sustainability of many SSPs where system maintenance is dependent on the balance between grazing pressure and regeneration or maintenance of trees and shrubs. Pastor.i is a smartphone application (APP) designed to allow pasture data logging to be very simple. The application is synchronized with the website and allows the producer to have in his pocket all the farm, being possible to identify the paddock, calculate the area, record the movements of the animals and consult the occupation history of the paddock. The application calculates the actual stocking rate, that can be associated with the location of the animals, obtained if the animals are using collars with GPS, which allows to know the areas of the paddock that are most grazed, visualized through heat maps. The information enables localized actions, such as fertilizing or sowing, to improve areas that are not grazed. The application also allows you to save photos of the sward. This temporal photographic record provides information on the condition of trees, the botanical composition and on the tendency of grazing to improve or to worsen coverage. The APP is available for download, is compatible with Android and is being tested with focus groups

Grazing in silvopastoral systems: multiple solutions for diversified benefits

Abstract Silvopastoral systems are multifunctional systems that combine herbage, shrub and tree layers with grazing animals in a single site. The multiple possible combinations of components within these systems creates different options that have in common the capacity to deliver positive outcomes related to land productivity and environmental and climatic benefits. This editorial provides a perspective of the diversity of ancient and more recent silvopastoral systems and their main benefits. The major challenges for both systems are different. Ancient silvopastoral systems, which originated on cultural grounds, deal mostly with conservation issues, while intensive silvopastoral systems, founded on technological changes of the grazing livestock production model, are more production driven but also concerned with climatic changes. Both types of system share similar benefits, and in this special issue we look at positive outcomes in the perspective of grazing animals, highlighting production and welfare. The purpose of this special issue is to contribute to gathering and sharing the knowledge emerging from grazing on different silvopastoral systems, promoting a common ground for future integrative research approaches

Systematic Review and Meta-Analysis

This study was supported by the European Regional Development Fund through the operation (POCI-01-0145-FEDER-007746) funded by the Programa Operacional Competitividade e Internacionalização–Compete2020 and by National Funds through Fundação para a Ciência e a Tecnologia within the Center for Health Technology and Services Research, R&D Unit (reference UID/IC/4255/2013).BACKGROUND: Prostate cancer is a leading cause of cancer among men. Because screening for prostate cancer is a controversial issue, many experts in the field have defended the use of shared decision making using validated decision aids, which can be presented in different formats (eg, written, multimedia, Web). Recent studies have concluded that decision aids improve knowledge and reduce decisional conflict. OBJECTIVE: This meta-analysis aimed to investigate the impact of using Web-based decision aids to support men's prostate cancer screening decisions in comparison with usual care and other formats of decision aids. METHODS: We searched PubMed, CINAHL, PsycINFO, and Cochrane CENTRAL databases up to November 2016. This search identified randomized controlled trials, which assessed Web-based decision aids for men making a prostate cancer screening decision and reported quality of decision-making outcomes. Two reviewers independently screened citations for inclusion criteria, extracted data, and assessed risk of bias. Using a random-effects model, meta-analyses were conducted pooling results using mean differences (MD), standardized mean differences (SMD), and relative risks (RR). RESULTS: Of 2406 unique citations, 7 randomized controlled trials met the inclusion criteria. For risk of bias, selective outcome reporting and participant/personnel blinding were mostly rated as unclear due to inadequate reporting. Based on seven items, two studies had high risk of bias for one item. Compared to usual care, Web-based decision aids increased knowledge (SMD 0.46; 95% CI 0.18-0.75), reduced decisional conflict (MD -7.07%; 95% CI -9.44 to -4.71), and reduced the practitioner control role in the decision-making process (RR 0.50; 95% CI 0.31-0.81). Web-based decision aids compared to printed decision aids yielded no differences in knowledge, decisional conflict, and participation in decision or screening behaviors. Compared to video decision aids, Web-based decision aids showed lower average knowledge scores (SMD -0.50; 95% CI -0.88 to -0.12) and a slight decrease in prostate-specific antigen screening (RR 1.12; 95% CI 1.01-1.25). CONCLUSIONS: According to this analysis, Web-based decision aids performed similarly to alternative formats (ie, printed, video) for the assessed decision-quality outcomes. The low cost, readiness, availability, and anonymity of the Web can be an advantage for increasing access to decision aids that support prostate cancer screening decisions among men.publishersversionpublishe

Fast prototyping microfluidics: Integrating droplet digital lamp for absolute quantification of cancer biomarkers

UID/CTM/50025/2019 UID/Multi/04378/2019 Inn-INDIGO/0002/2015 PTDC/BTM-SAL/31201/2017 SFRH/BPD/124311/2016Microfluidic (MF) advancements have been leveraged toward the development of state-of-the-art platforms for molecular diagnostics, where isothermal amplification schemes allow for further simplification of DNA detection and quantification protocols. The MF integration with loop-mediated isothermal amplification (LAMP) is today the focus of a new generation of chip-based devices for molecular detection, aiming at fast and automated nucleic acid analysis. Here, we combined MF with droplet digital LAMP (ddLAMP) on an all-in-one device that allows for droplet generation, target amplification, and absolute quantification. This multilayer 3D chip was developed in less than 30 minutes by using a low-cost and extremely adaptable production process that exploits direct laser writing technology in “Shrinky-dinks” polystyrene sheets. ddLAMP and target quantification were performed directly on-chip, showing a high correlation between target concentration and positive droplet score. We validated this integrated chip via the amplification of targets ranging from five to 500,000 copies/reaction. Furthermore, on-chip amplification was performed in a 10 µL volume, attaining a limit of detection of five copies/µL under 60 min. This technology was applied to quantify a cancer biomarker, c-MYC, but it can be further extended to any other disease biomarker.publishersversionpublishe

Tannins in Ruminant Nutrition: Impact on Animal Performance and Quality of Edible Products

Tannins are widespread throughout the plant kingdom, occurring as hydrolysable and condensed tannins and at different levels in several animal feeding sources. Recent years have seen an increasing interest in the use of tannin-rich plants and plant extracts in ruminant diets for improving the quality of their edible products. Some results show that this strategy is effective in improving the fatty acid profile of meat and milk, increasing the level of health-beneficial fatty acids as well as enhancing the oxidative stability of the products. However, the use of tannin-rich feed in animal diets requires great care, due to its possible detrimental effects on animal performance and induction of metabolic disorders. Although promising, the results of studies on the effects of tannins on animal performance and quality of their products are still controversial, probably depending on type and chemical structure of tannins, amount ingested, composition of diet, and species of animal. In this chapter, the current knowledge regarding the effect of dietary tannins on animal performance and the quality of their products (meat and milk), particularly on the fatty acid profile, oxidative stability, and organoleptic properties, is reviewed. The tannin chemistry diversity and its occurrence in ruminant diets, as well as its beneficial and adverse effects on ruminants, will be briefly reviewed, and aspects related to oral cavity physiology, saliva production/composition, and postingestive effects will also be discussed

Paper-Based SERS Platform for One-Step Screening of Tetracycline in Milk

PTDC/CTM-NAN/2912/2014. PTDC/AAG-TEC/5400/2014. POCI-01-0145-FEDER-016637. POCI-01-0145-FEDER-007688. UID/CTM/50025/2019. FCT/MCTES SFRH/BD/115173/2016 and SFRH/BPD/124311/2016 .Throughout the last decade, the expansion of food testing has been gradually moving towards ordinary high throughput screening methods performed on-site. The demand for point-of-care testing, able to distinguish molecular signatures with high accuracy, sensitivity and specificity has been significantly increasing. This new requirement relies on the on-site detection and monitorization of molecular signatures suitable for the surveillance of food production and processing. The widespread use of antibiotics has contributed to disease control of livestock but has also created problems for the dairy industry and consumers. Its therapeutic and subtherapeutic use has increased the risk of contamination in milk in enough concentrations to cause economic losses to the dairy industry and have a health impact in highly sensitive individuals. This study focuses on the development of a simple Surface-Enhanced Raman Spectroscopy (SERS) method for fast high throughput screening of tetracycline (TET) in milk. For this, we integrate a paper-based low-cost, fully recyclable and highly stable SERS platform, with a minimal sample preparation protocol. A two-microliter sample of milk solutions spiked with TET (from 0.01 to 1000 ppm) is dried on a silver nanoparticle coated cardboard substrate and measured via a Raman spectrophotometer. The SERS substrate showed to be extremely stable with a shelf life of several months. A global spectrum principal component analysis approach was used to test all the detected vibrational modes and their correlation with TET concentration. Peak intensity ratios (455 cm−1/1280 cm−1 and 874 cm−1/1397 cm−1) were found to be correlated with TET concentrations in milk, achieving a sensitivity as low as 0.1 ppm. Results indicate that this SERS method combined with portable Raman spectrometer is a potential tool that can be used on-site for the monitoring of TET residues and other antibiotics.publishersversionpublishe

Deacetylation and Desuccinylation of the Fucose-Rich Polysaccharide Fucopol: Impact on Biopolymer Physical and Chemical Properties

FucoPol is an acylated polysaccharide with demonstrated valuable functional properties that include a shear thinning fluid behaviour, a film-forming capacity, and an emulsion forming and stabilizing capacity. In this study, the different conditions (concentration, temperature, and time) for alkaline treatment were investigated to deacylate FucoPol. Complete deacetylation and desuccinylation was achieved with 0.02 M NaOH, at 60 ºC for 15 min, with no significant impact on the biopolymer’s sugar composition, pyruvate content, and molecular mass distribution. FucoPol depyruvylation by acid hydrolysis was attempted, but it resulted in a very low polymer recovery. The effect of the ionic strength, pH, and temperature on the deacetylated/desuccinylated polysaccharide, d-FucoPol, was evaluated, as well as its emulsion and film-forming capacity. d-FucoPol aqueous solutions maintained the shear thinning behaviour characteristic of FucoPol, but the apparent viscosity decreased significantly. Moreover, contrary to FucoPol, whose solutions were not affected by the media’s ionic strength, the d-FucoPol solutions had a significantly higher apparent viscosity for a higher ionic strength. On the other hand, the d-FucoPol solutions were not affected by the pH in the range of 3.6–11.5, while FucoPol had a decreased viscosity for acidic pH values and for a pH above 10.5. Although d-FucoPol displayed an emulsification activity for olive oil similar to that of FucoPol (98 +- 0%) for an oil-to-water ratio of 2:3, the emulsions were less viscous. The d-FucoPol films were flexible, with a higher Young0s modulus (798 +- 152 MPa), a stress at the break (22.5 +- 2.5 MPa), and an elongation at the break (9.3 +- 0.7%) than FucoPol (458 +- 32 MPa, 15.5 +- 0.3 MPa and 8.1 +- 1.0%, respectively). Given these findings, d-FucoPol arises as a promising novel biopolymer, with distinctive properties that may render it useful for utilization as a suspending or emulsifier agent, and as a barrier in coatings and packaging filmsinfo:eu-repo/semantics/publishedVersio

Changes in mouse whole saliva soluble proteome induced by tannin-enriched diet

<p>Abstract</p> <p>Background</p> <p>Previous studies suggested that dietary tannin ingestion may induce changes in mouse salivary proteins in addition to the primarily studied proline-rich proteins (PRPs). The aim of the present study was to determine the protein expression changes induced by condensed tannin intake on the fraction of mouse whole salivary proteins that are unable to form insoluble tannin-protein complexes. Two-dimensional polyacrylamide gel electrophoresis protein separation was used, followed by protein identification by mass spectrometry.</p> <p>Results</p> <p>Fifty-seven protein spots were excised from control group gels, and 21 different proteins were identified. With tannin consumption, the expression levels of one α-amylase isoform and one unidentified protein increased, whereas acidic mammalian chitinase and Muc10 decreased. Additionally, two basic spots that stained pink with Coomassie Brilliant Blue R-250 were newly observed, suggesting that some induced PRPs may remain uncomplexed or form soluble complexes with tannins.</p> <p>Conclusion</p> <p>This proteomic analysis provides evidence that other salivary proteins, in addition to tannin-precipitating proteins, are affected by tannin ingestion. Changes in the expression levels of the acidic mammalian chitinase precursor and in one of the 14 salivary α-amylase isoforms underscores the need to further investigate their role in tannin ingestion.</p

Sheep and goat saliva proteome analysis: A useful tool for ingestive behavior research?

Sheep and goats differ in diet selection, which may reflect different abilities to deal with the ingestion of plant secondary metabolites. Although saliva provides a basis for immediate oral information via sensory cues and also a mechanism for detoxification, our understanding of the role of saliva in the pre-gastric control of the intake of herbivores is rudimentary. Salivary proteins have important biological functions, but despite their significance, their expression patterns in sheep and goats have been little studied. Protein separation techniques coupled to mass spectrometry based techniques have been used to obtain an extensive comprehension of human saliva protein composition but far fewer studies have been undertaken on animals' saliva. We used two-dimensional electrophoresis gel analysis to compare sheep and goats parotid saliva proteome. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) and liquid chromatography tandem mass spectrometry (LC-MS/MS) were used to identify proteins. From a total of 260 sheep and 205 goat saliva protein spots, 117 and 106 were identified, respectively. A high proportion of serum proteins were found in both salivary protein profiles. Major differences between the two species were detected for proteins within the range of 25–35 kDa. This study presents the parotid saliva proteome of sheep and goat and highlights the potential of proteomics for investigation relating to intake behavior research

Result-based payments as a tool to preserve the High Nature Value of complex silvo-pastoral systems: progress towards farm-based indicators

s shown by the Green Deal's ambition, the European Commission is progressively pushing for an environmental shiftand climate action in Europe. For the Common Agricultural Policy (CAP), this involves a stronger focus on greening policy objectives.For agri-environmental schemes, this entails changes toward performance-based payments, partially replacing traditional activity-basedpayments. The CAP foresees greater flexibility in national programs and tailor-made solutions centered on results (i.e. environmentaloutcomes), benefiting farmers who go beyond the minimum environmental performance required. The environmental outcomes offarm practices must be assessed so that changes can be monitored over time and linked to payment delivery. This requires stakeholdersto collaborate with researchers to identify farm-based indicators that are easily applicable, to achieve environmental results that aredependent on farm practices, and to assess and monitor changes in outcomes over time. The analysis in this paper is based on atransdisciplinary process that began in 2017 in a Natura 2000 site and its surroundings in Southern Portugal, to identify result-basedmeasures for the Montado silvo-pastoral system. Farmers' understanding of how to adapt their practices to reach better environmentalresults was combined with scientific knowledge of the relevant environmental outcomes and how these can be assessed with indicators.Ten field-based visual indicators were defined, which farmers applied in the field, and validated by technical staff. These indicators arerelated to several aspects of the silvo-pastoral system: soil quality, pasture diversity, tree renewal, tree health, singular landscape elements,and biodiversity. The approach used in this process was innovative. We describe each step and present its advantages and drawbacksfor designing and implementing result-based payments. Ultimately, their implementation is expected to lead to higher sustainability inthe Montado. (PDF) Result-based payments as a tool to preserve the High Nature Value of complex silvo-pastoral systems: progress toward farm-based indicators. Available from: https://www.researchgate.net/publication/359442049_Result-based_payments_as_a_tool_to_preserve_the_High_Nature_Value_of_complex_silvo-pastoral_systems_progress_toward_farm-based_indicators#fullTextFileContent [accessed Feb 03 2023]