3 research outputs found
1.4 nm gold nanoparticle-antibody conjugates for in situ gold immunolabelling after transduction into living human cells
Despite advances in Electron Microscopy (EM) that enable to image protein assemblies within vitreous sections of cells at nearly atomic resolution, labelling is still necessary to locate small proteins or rare complexes. Gold immunolabelling has been used for decades to localise specific proteins within cellular sections. However, current gold particle-antibody conjugates are not built with enough chemical precision to match the current resolution offered by cryo-EM methodology. Furthermore, as a close to native specimen state can only be achieved by strict preservation of a frozen hydrated state, it is required to deliver gold labelling agents into living cells prior to their vitrification. Several 1.4Â nm gold nanoparticle-antibody conjugates were synthesised. Their abilities to bind to and label their corresponding epitopes within living cells after cytosolic delivery by electroporation are documented here
1.4 nm gold nanoparticle-antibody conjugates for in situ gold immunolabelling after transduction into living human cells
Bioactivated and PEG-Protected Circa 2 nm Gold Nanoparticles for in Cell Labelling and Cryo-Electron Microscopy
Advances in cryo-electron microscopy (EM) enable imaging of protein assemblies within mammalian cells in a near native state when samples are preserved by cryogenic vitrification. To accompany this progress, specialized EM labelling protocols must be developed. Gold nanoparticles (AuNPs) of 2Â nm are synthesized and functionalized to bind selected intracellular targets inside living human cells and to be detected in vitreous sections. As a proof of concept, thioaminobenzoate-, thionitrobenzoate-coordinated gold nanoparticles are functionalized on their surface with SV40 Nuclear Localization Signal (NLS)-containing peptides and 2Â kDa polyethyleneglycols (PEG) by thiolate exchange to target the importin-mediated nuclear machinery and facilitate cytosolic diffusion by shielding the AuNP surface from non-specific binding to cell components, respectively. After delivery by electroporation into the cytoplasm of living human cells, the PEG-coated AuNPs diffuse freely in the cytoplasm but do not enter the nucleus. Incorporation of NLS within the PEG coverage promotes a quick nuclear import of the nanoparticles in relation to the density of NLS onto the AuNPs. Cryo-EM of vitreous cell sections demonstrate the presence of 2Â nm AuNPs as single entities in the nucleus. Biofunctionalized AuNPs combined with live-cell electroporation procedures are thus potent labeling tools for the identification of macromolecules in cellular cryo-EM