NEUROREGENERATIVE EFFECTS OF D-α-TOCOPHEROL ON THE CRUSHED SCIATIC NERVE OF DIABETIC RATS

ABSTRACTObjective: This present study is designed to explore the neuroregenerative effects of d-α-tocopherol on peripheral nerve in both healthy and diabeticrats.Methods: 24 albino rats were divided into four groups; healthy control, diabetic control, healthy treated, and diabetic treated. Diabetes was inducedby single subcutaneous injection of alloxan (100 mg/kg). Treated groups were administered d-α-tocopherol orally, daily (200 mg/kg) for 3 weeks.Through a mid-thigh incision, sciatic nerve was crushed with Kocher's forceps, and skin wound was closed with absorbable suture. Sciatic functionaland static indices were used to assess the functional recovery in conjunction with histological, histomorphological, and biochemical analyses at theend of 3 weeks.Results: It was noticed that d-α-tocopherol supplementation accelerates functional recovery, enhances the antioxidant levels, and promotes theregeneration and extracellular matrix reorganization of peripheral nerves.Conclusion: It is concluded that the d-α-tocopherol appears to be an optimistic option in the management of peripheral nerve crush injury in bothhealthy and diabetics.Keywords: Antioxidant, d-α-tocopherol, Diabetes, Rats, Regeneration, Sciatic nerve

Histopathological Study of Experimentally Crushed Skeletal Muscle’s Regeneration in Adult Albino Rats

Aim: This study aims to explain all the events of skeletal muscle repair and regeneration with the help of suitable histophathlogical photomicrographs taken from crush-injured adult albino rat’s gluteus maximus muscle. Materials and Methods: The present study is part of our previous research study related to skeletal muscle repair and regeneration in crush injured gluteus maximus muscle of adult albino rats. The samples were processed for histopathological examination using routine and special histological staining procedures. The tissue samples were examined under trinocular microscope, and the fields showing interesting findings were recorded under different magnification. Results: In this study we observed all regenerative changes in myofibers and related structures after crushed injury. Conclusion: Histopathological studies with good stainings are helpful for the easy identification of minute changes that occurs in each stages of skeletal muscle regeneration

Eff ect of vitamin E isoforms on the primary intention Eff ect of vitamin E isoforms on the primary intention skin wound healing of diabetic rats

Introduction: Impaired wound healing events is a common complication in diabetes. One of the effective nutritional antioxidant on skin wound healing is vitamin E which contains saturated tocopherol and unsaturated tocotrienol forms. This present study is designed to explore the effect of different vitamin E isoforms on stitched skin wound in both healthy and diabetic rats. Materials and Methods: Forty eight albino rats were divided into eight groups; healthy control, diabetic control, healthy treated (d-α-tocopherol, d-δ-TRF and co-administrated) and diabetic treated (d-αtocopherol, d-δ-TRF and co-administrated). Diabetes was induced through single subcutaneous injection of alloxan at the dose of 100 mg/kg. Treated groups were administered d-a-tocopherol (200 mg/kg), d-δ-TRF (200 mg/kg) and co-administration (100 mg/kg of these two compounds each) orally and daily for three weeks. A horizontal skin incision was made on right mid-thigh region at 2.95 ± 0.17cm in length and wound was closed with an absorbable suture. Results: Histopathological and histomorphological results at the end of 3rd week revealed that the d-δ-TRF treated groups promote the regeneration and reorganization of epidermal and dermal components in healing of primary intention more effectively than the d-α-tocopherol and co-administrated groups. Conclusion: It is concluded that among different vitamin E isoforms the d-δ-TRF appears to be a more effective nutritional antioxidant on skin wound healing in both healthy and diabetics

Th erapeutic potential of d-Th erapeutic potential of d-δ-tocotrienol rich fraction -tocotrienol rich fraction on excisional skin wounds in diabetic rats

Introduction: Long-standing hyperglycemia in addition to many of its associated complications also hampers normal wound healing which may be further aggravated in the presence of infection and oxidative stress. Therefore, antioxidant supplementation appears to be strategically relevant for wound healing. This study is designed to explore the therapeutic potential of d-δ-tocotrienol rich fraction (d-δ-TRF) on skin wound healing in both healthy and diabetic rats. Materials and Methods: Diabetes was induced through single subcutaneous injection of alloxan at the dose of 100 mg/kg at hip region. 24 albino rats were divided into four groups; healthy control, diabetic control, healthy treated and diabetic treated. d-δ-TRF was administered to treated groups (200 mg/kg), orally, daily for 3 weeks. Full thickness excisional skin wounds were. Wound area was studied by assessing the morphological, histomorphological and histological features at weekly intervals and biochemical analyses were performed at the end of 3rd week. Results: The findings of present study revealed that d-δ-TRF accelerated the skin wound healing by means of early regeneration of both epidermal and dermal components; enhancement of serum protein synthesis, improvement of antioxidant status, maintenance of glycemic condition and controlling serum creatinine levels in diabetic rats. Conclusion: It is concluded that d-δ-TRF has significant therapeutic potency on the healing of skin wounds in both healthy and diabetics