Examining the Effect of Pore Size Distribution and Shape on Flow through Unsaturated Peat using Computer Tomography

The hydraulic conductivity of unsaturated peat soil is controlled by the air-filled porosity, pore size and geometric distribution as well as other physical properties of peat materials. This study investigates how the size and shape of pores affects the flow of water through peat soils. In this study we used X-ray Computed Tomography (CT), at 45μm resolution under 5 specific soil-water pressure head levels to provide 3-D, high-resolution images that were used to detect the inner pore structure of peat samples under a changing water regime. Pore structure and configuration were found to be irregular, which affected the rate of water transmission through peat soils. The 3-D analysis suggested that pore distribution is dominated by a single large pore-space. At low pressure head, this single large air-filled pore imparted a more effective flowpath compared to smaller pores. Smaller pores were disconnected and the flowpath was more tortuous than in the single large air-filled pore, and their contribution to flow was negligible when the single large pore was active. We quantify the pore structure of peat soil that affects the hydraulic conductivity in the unsaturated condition, and demonstrate the validity of our estimation of peat unsaturated hydraulic conductivity by making a comparison with a standard permeameter-based method. Estimates of unsaturated hydraulic conductivities were made for the purpose of testing the sensitivity of pore shape and geometry parameters on the hydraulic properties of peats and how to evaluate the structure of the peat and its affects on parameterization. We also studied the ability to quantify these factors for different soil moisture contents in order to define how the factors controlling the shape coefficient vary with changes in soil water pressure head. The relation between measured and estimated unsaturated hydraulic conductivity at various heads shows that rapid initial drainage, that changes the air-filled pore properties, creates a sharp decline in hydraulic conductivity. This is because the large pores readily lose water, the peat rapidly becomes less conductive and the flow path among pores, more tortuous

Subsidence across the Antler foreland of Montana and Idaho: Tectonic versus eustatic effects

Devonian and Mississippian sedimentary rocks of western Montana and east-central Idaho were deposited on a cratonic platform that faced a deep basin to the west. The deep basin in Idaho was a northern extension of the Antler foredeep and formed as a flexural response to loading of the ancient North American continental margin by an inferred arc and thrust belt complex. Subsidence analyses of the Devonian-Mississippian strata indicate episodic subsidence events in the proximal foredeep and adjacent cratonic platform, an area approximately 800 km (500 mi) wide (palinspastic). Isopach maps for this sequence illustrate that many depocenters and paleohighs were geographically coincident across the foreland through time. The Devonian-Mississippian foreland structures had cross-sectional wavelengths of 50-200 km (30-120 mi) and amplitudes of about 50-350 m (160-1,150 ft). Some of these structures were tectonically inverted (i.e., paleohighs became depocenters and vice versa) several times during the 50-60 m.y. represented by this stratigraphic sequence. Many of these generally east-west-trending paleostructures were oriented at high angles to the north-south-trending axis of the Antler foredeep and the inferred strike of the Antler orogenic belt. These foreland structures coincide geographically with structural trends produced during Proterozoic extension, suggesting that the Proterozoic faults were reactivated during Antler convergence. The isopach maps also show progressive southeastward migration of Antler foredeep depocenters from Late Devonian to Early Pennsylvanian time. The southeastward migration of the foredeep depocenter suggests that the maximum thrust load moved progressively southeastward from Late Devonian to Early Pennsylvanian time. The complex patterns of subsidence across the Montana-Idaho foreland do not fit into simple flexural models for vertical loading of unbroken elastic plates. Instead, differential subsidence of the foreland may be related to several mechanisms: (1) flexure of mechanically independent, fault-bounded segments of the foreland produced by areally limited thrust loads (subregional vertical loading); (2) transmission of compressive in-plane stresses through the foreland lithosphere (regional horizontal loading) that may have reactivated Proterozoic fault systems; and (3) waxing and waning of in-plane compressive stresses resulting from the episodic nature of Antler convergence. Results from this study suggest that, in settings where the foreland lithosphere is broken by ancient fault systems, the foreland may exhibit complex patterns of differential subsidence that probably reflect a composite response to both vertical and horizontal loads. Also, the simultaneous pulses of subsidence documented across large parts of the Antler foreland suggest that it may be possible to date episodes of convergence along ancient continental margins, even when the ancient thrust belt complex is poorly preserved

Subsidence across the Antler foreland of Montana and Idaho: Tectonic versus eustatic effects

Devonian and Mississippian sedimentary rocks of western Montana and east-central Idaho were deposited on a cratonic platform that faced a deep basin to the west. The deep basin in Idaho was a northern extension of the Antler foredeep and formed as a flexural response to loading of the ancient North American continental margin by an inferred arc and thrust belt complex. Subsidence analyses of the Devonian-Mississippian strata indicate episodic subsidence events in the proximal foredeep and adjacent cratonic platform, an area approximately 800 km (500 mi) wide (palinspastic). Isopach maps for this sequence illustrate that many depocenters and paleohighs were geographically coincident across the foreland through time. The Devonian-Mississippian foreland structures had cross-sectional wavelengths of 50-200 km (30-120 mi) and amplitudes of about 50-350 m (160-1,150 ft). Some of these structures were tectonically inverted (i.e., paleohighs became depocenters and vice versa) several times during the 50-60 m.y. represented by this stratigraphic sequence. Many of these generally east-west-trending paleostructures were oriented at high angles to the north-south-trending axis of the Antler foredeep and the inferred strike of the Antler orogenic belt. These foreland structures coincide geographically with structural trends produced during Proterozoic extension, suggesting that the Proterozoic faults were reactivated during Antler convergence. The isopach maps also show progressive southeastward migration of Antler foredeep depocenters from Late Devonian to Early Pennsylvanian time. The southeastward migration of the foredeep depocenter suggests that the maximum thrust load moved progressively southeastward from Late Devonian to Early Pennsylvanian time. The complex patterns of subsidence across the Montana-Idaho foreland do not fit into simple flexural models for vertical loading of unbroken elastic plates. Instead, differential subsidence of the foreland may be related to several mechanisms: (1) flexure of mechanically independent, fault-bounded segments of the foreland produced by areally limited thrust loads (subregional vertical loading); (2) transmission of compressive in-plane stresses through the foreland lithosphere (regional horizontal loading) that may have reactivated Proterozoic fault systems; and (3) waxing and waning of in-plane compressive stresses resulting from the episodic nature of Antler convergence. Results from this study suggest that, in settings where the foreland lithosphere is broken by ancient fault systems, the foreland may exhibit complex patterns of differential subsidence that probably reflect a composite response to both vertical and horizontal loads. Also, the simultaneous pulses of subsidence documented across large parts of the Antler foreland suggest that it may be possible to date episodes of convergence along ancient continental margins, even when the ancient thrust belt complex is poorly preserved

BCR-ABL activity and its response to drugs can be determined in CD34+ CML stem cells by CrkL phosphorylation status using flow cytometry.

In chronic myeloid leukaemia, CD34(+) stem/progenitor cells appear resistant to imatinib mesylate (IM) in vitro and in vivo. To investigate the underlying mechanism(s) of IM resistance, it is essential to quantify Bcr-Abl kinase status at the stem cell level. We developed a flow cytometry method to measure CrkL phosphorylation (P-CrkL) in samples with <10(4) cells. The method was first validated in wild-type (K562) and mutant (BAF3) BCR-ABL(+) as well as BCR-ABL(-) (HL60) cell lines. In response to increasing IM concentration, there was a linear reduction in P-CrkL, which was Bcr-Abl specific and correlated with known resistance. The results were comparable to those from Western blotting. The method also proved to be reproducible with small samples of normal and Ph(+) CD34(+) cells and was able to discriminate between Ph(-), sensitive and resistant Ph(+) cells. This assay should now enable investigators to unravel the mechanism(s) of IM resistance in stem cells

Nonequilibrium fluctuation dissipation relations of interacting Brownian particles driven by shear

We present a detailed analysis of the fluctuation dissipation theorem (FDT) close to the glass transition in colloidal suspensions under steady shear using mode coupling approximations. Starting point is the many-particle Smoluchowski equation. Under shear, detailed balance is broken and the response functions in the stationary state are smaller at long times than estimated from the equilibrium FDT. An asymptotically constant relation connects response and fluctuations during the shear driven decay, restoring the form of the FDT with, however, a ratio different from the equilibrium one. At short times, the equilibrium FDT holds. We follow two independent approaches whose results are in qualitative agreement. To discuss the derived fluctuation dissipation ratios, we show an exact reformulation of the susceptibility which contains not the full Smoluchowski operator as in equilibrium, but only its well defined Hermitian part. This Hermitian part can be interpreted as governing the dynamics in the frame comoving with the probability current. We present a simple toy model which illustrates the FDT violation in the sheared colloidal system.Comment: 21 pages, 13 figures, submitted to Phys. Rev.

Multi-processing CTH: Porting legacy FORTRAN code to MP hardware

CTH is a family of codes developed at Sandia National Laboratories for use in modeling complex multi-dimensional, multi-material problems that are characterized by large deformations and/or strong shocks. A two-step, second-order accurate Eulerian solution algorithm is used to solve the mass, momentum, and energy conservation equations. CTH has historically been run on systems where the data are directly accessible to the cpu, such as workstations and vector supercomputers. Multiple cpus can be used if all data are accessible to all cpus. This is accomplished by placing compiler directives or subroutine calls within the source code. The CTH team has implemented this scheme for Cray shared memory machines under the Unicos operating system. This technique is effective, but difficult to port to other (similar) shared memory architectures because each vendor has a different format of directives or subroutine calls. A different model of high performance computing is one where many (> 1,000) cpus work on a portion of the entire problem and communicate by passing messages that contain boundary data. Most, if not all, codes that run effectively on parallel hardware were written with a parallel computing paradigm in mind. Modifying an existing code written for serial nodes poses a significantly different set of challenges that will be discussed. CTH, a legacy FORTRAN code, has been modified to allow for solutions on distributed memory parallel computers such as the IBM SP2, the Intel Paragon, Cray T3D, or a network of workstations. The message passing version of CTH will be discussed and example calculations will be presented along with performance data. Current timing studies indicate that CTH is 2--3 times faster than equivalent C++ code written specifically for parallel hardware. CTH on the Intel Paragon exhibits linear speed up with problems that are scaled (constant problem size per node) for the number of parallel nodes

Analytical Results for a Hole in an Antiferromagnet

The Green's function for a hole moving in an antiferromagnet is derived analytically in the long-wavelength limit. We find that the infrared divergence is eliminated in two and higher dimensions so that the quasiparticle weight is finite. Our results also suggest that the hole motion is polaronic in nature with a bandwidth proportional to $t/J \exp [-c (t/J)^2]$ ($c$ is a constant). The connection of the long-wavelength approximation to the first-order approximation in the cumulant expansion is also clarified.Comment: 12 papes, 2 figures available upon request, revte

Anti-inflammatory activity and neutrophil reductions mediated by the JAK1/JAK3 inhibitor, CP-690,550, in rat adjuvant-induced arthritis

<p>Abstract</p> <p>Background</p> <p>The Janus kinase (JAK) family of tyrosine kinases includes JAK1, JAK2, JAK3 and TYK2, and is required for signaling through Type I and Type II cytokine receptors. CP-690,550 is a potent and selective JAK inhibitor currently in clinical trials for rheumatoid arthritis (RA) and other autoimmune disease indications. In RA trials, dose-dependent decreases in neutrophil counts (PBNC) were observed with CP-690,550 treatment. These studies were undertaken to better understand the relationship between JAK selectivity and PBNC decreases observed with CP-690,550 treatment.</p> <p>Methods</p> <p>Potency and selectivity of CP-690,550 for mouse, rat and human JAKs was evaluated in a panel of <it>in vitro </it>assays. The effect of CP-690,550 on granulopoiesis from progenitor cells was also assessed <it>in vitro </it>using colony forming assays. <it>In vivo </it>the potency of orally administered CP-690,550 on arthritis (paw edema), plasma cytokines, PBNC and bone marrow differentials were evaluated in the rat adjuvant-induced arthritis (AIA) model.</p> <p>Results</p> <p>CP-690,550 potently inhibited signaling through JAK1 and JAK3 with 5-100 fold selectivity over JAK2 in cellular assays, despite inhibiting all four JAK isoforms with nM potency in <it>in vitro </it>enzyme assays. Dose-dependent inhibition of paw edema was observed <it>in vivo </it>with CP-690,550 treatment. Plasma cytokines (IL-6 and IL-17), PBNC, and bone marrow myeloid progenitor cells were elevated in the context of AIA disease. At efficacious exposures, CP-690,550 returned all of these parameters to pre-disease levels. The plasma concentration of CP-690,550 at efficacious doses was above the <it>in vitro </it>whole blood IC50 of JAK1 and JAK3 inhibition, but not that of JAK2.</p> <p>Conclusion</p> <p>Results from this investigation suggest that CP-690,550 is a potent inhibitor of JAK1 and JAK3 with potentially reduced cellular potency for JAK2. In rat AIA, as in the case of human RA, PBNC were decreased at efficacious exposures of CP-690,550. Inflammatory end points were similarly reduced, as judged by attenuation of paw edema and cytokines IL-6 and IL-17. Plasma concentration at these exposures was consistent with inhibition of JAK1 and JAK3 but not JAK2. Decreases in PBNC following CP-690,550 treatment may thus be related to attenuation of inflammation and are likely not due to suppression of granulopoiesis through JAK2 inhibition.</p