A new eudesmane derivative from Leontodon tuberosus

Besides the known compounds 2,4,6-trihydroxyacetophenone 4-O-β -D-glucopyranoside and syringaresinol 4'-O-β -D-glucopyranoside, the novel sesquiterpenoid 1,2-dehydro-3-oxocostic acid β -D-glucopyranoside ester was isolated from Leontodon tuberosus L. and its structure established by mass spectrometry and 1D-and 2D-NMR spectroscopy. Additionally, a number of fatty and phenolic acids was identified in the crude methanolic extract by HPLC-DAD and HPLC-MS. The chemosystematic impact of the new sesquiterpenoid is discussed briefly

A prolonged run-in period of standard subcutaneous microdialysis ameliorates quality of interstitial glucose signal in patients after major cardiac surgery

We evaluated a standard subcutaneous microdialysis technique for glucose monitoring in two critically ill patient populations and tested whether a prolonged run-in period improves the quality of the interstitial glucose signal. 20 surgical patients after major cardiac surgery (APACHE II score: 10.1 ± 3.2) and 10 medical patients with severe sepsis (APACHE II score: 31.1 ± 4.3) were included in this investigation. A microdialysis catheter was inserted in the subcutaneous adipose tissue of the abdominal region. Interstitial fluid and arterial blood were sampled in hourly intervals to analyse glucose concentrations. Subcutaneous adipose tissue glucose was prospectively calibrated to reference arterial blood either at hour 1 or at hour 6. Median absolute relative difference of glucose (MARD), calibrated at hour 6 (6.2 (2.6; 12.4) %) versus hour 1 (9.9 (4.2; 17.9) %) after catheter insertion indicated a significant improvement in signal quality in patients after major cardiac surgery (p < 0.001). Prolonged run-in period revealed no significant improvement in patients with severe sepsis, but the number of extreme deviations from the blood plasma values could be reduced. Improved concurrence of glucose readings via a 6-hour run-in period could only be achieved in patients after major cardiac surgery

Albumin and mammalian cell culture: implications for biotechnology applications

Albumin has a long historical involvement in design of media for the successful culture of mammalian cells, in both the research and commercial fields. The potential application of albumins, bovine or human serum albumin, for cell culture is a by-product of the physico-chemical, biochemical and cell-specific properties of the molecule. In this review an analysis of these features of albumin leads to a consideration of the extracellular and intracellular actions of the molecule, and importantly the role of its interactions with numerous ligands or bioactive factors that influence the growth of cells in culture: these include hormones, growth factors, lipids, amino acids, metal ions, reactive oxygen and nitrogen species to name a few. The interaction of albumin with the cell in relation to these co-factors has a potential impact on metabolic and biosynthetic activity, cell proliferation and survival. Application of this knowledge to improve the performance in manufacturing biotechnology and in the emerging uses of cell culture for tissue engineering and stem cell derived therapies is an important prospect

JSCS–4002 Original scientific paper Rhamnopyranosylvitexin

Abstract: A methanolic extract of Celtis australis leaves yielded 2’’-α-rhamnopyranosylvitexin and 2’’-α-rhamnopyranosyl-7-O-methylvitexin. Both compounds are known from other sources from earlier investigations but the full NMR data for the latter compound are reported for the first time

On the occurrence of sonchuside A in Cicerbita alpina and its chemosystematic significance

The subaerial parts of Cicerbita alpina yielded the guaianolide 8-acetyl-15b-D-glucopyranosyllactucin (1) and the germacranolide Sonchuside A (2). The sonchuside A is reported for the first time from the genus Cicerbita. 1H-NMR and 13C NMR data of compounds 1 and 2 measured in deuterodimethyl sulfoxide and deuteromethanol, respectively, are given and the chemosystematic implications of the occurrence of sonchuside A in C. alpina are discussed briefly

A new trisaccharide derivative from Prenanthes purpurea

A methanolic extract of Prenanthes purpurea L. leaves yielded 1,6"- -di-O-cinnamoyl-β-D-glucopyranosyl-(1→3)-O-α-L-rhamnopyranosyl- (1→6)- -O-β-D-glucopyranoside. The NMR and physical data of this new natural compound are reported.PeerReviewe