The E5 protein of BPV-4 interacts with the heavy chain of MHC class I and irreversibly retains the MHC complex in the Golgi apparatus

BPV-4 E5 inhibits transcription of the bovine MHC class I heavy chain (HC) gene, increases degradation of HC and downregulates surface expression of MHC class I by retaining the complex in the Golgi apparatus (GA). Here we report that transcription inhibition can be alleviated by interferon treatment and the degradation of HC can be reversed by treatment with inhibitors of proteasomes and lysosomes. However, the inhibition of transport of MHC class I to the cell surface is irreversible. We show that E5 is capable of physically interacting with HC. Together with the inhibition of the vacuolar ATPase (due to the interaction between E5 and 16k subunit c), the interaction between E5 and HC is likely to be responsible for retention of MHC class I in the GA. C-terminus deletion mutants of E5 are incapable of either downregulating surface MHC class I or interacting with HC, establishing that the C-terminus domain of E5 is important in the inhibition of MHC class I

The E5 oncoprotein of BPV-4 does not interfere with the biosynthetic pathway of non-classical MHC class I

The major histocompatibility complex (MHC) class I region in mammals contains both classical and non-classical MHC class I genes. Classical MHC class I molecules present antigenic peptides to cytotoxic T lymphocytes, whereas non-classical MHC class I molecules have a variety of functions. Both classical and non-classical MHC molecules interact with natural killer cell receptors and may under some circumstances prevent cell death by natural killer cytotoxicity. The E5 oncoprotein of BPV-4 down-regulates the expression of classical MHC class I on the cell surface and retains the complex in the Golgi apparatus. The inhibition of classical MHC class I to the cell surface results from both the impaired acidification of the Golgi, due to the interaction of E5 with subunit c of the H+ V-ATPase, and to the physical binding of E5 to the heavy chain of MHC class I. Despite the profound effect of E5 on classical MHC class I, E5 does not retain a non-classical MHC class I in the Golgi, does not inhibit its transport to the cell surface and does not bind its heavy chain. We conclude that, as is the case for HPV-16 E5, BPV-4 E5 does not down-regulate certain non-classical MHC class I, potentially providing a mechanism for the escape of the infected cell from attack by both cytotoxic T lymphocytes and NK cells

Hidden talents in context: Cognitive performance with abstract versus ecological stimuli among adversity-exposed youth

Adversity-exposed youth tend to score lower on cognitive tests. However, the hidden talents approach proposes some abilities are enhanced by adversity, especially under ecologically relevant conditions. Two versions of an attention-shifting and working memory updating task—one abstract, one ecological—were administered to 618 youth (Mage = 13.62, SDage = 0.81; 48.22% female; 64.56% White). Measures of environmental unpredictability, violence, and poverty were collected to test adversity × task version interactions. There were no interactions for attention shifting. For working memory updating, youth exposed to violence and poverty scored lower than their peers with abstract stimuli but almost just as well with ecological stimuli. These results are striking compared to contemporary developmental science, which often reports lowered performance among adversity-exposed youth

Gauge-invariant gravitational wave modes in pre-big bang cosmology

The t<0 branch of pre-big bang cosmological scenarios is subject to a gravitational wave instability. The unstable behaviour of tensor perturbations is derived in a very simple way in Hwang's covariant and gauge-invariant formalism developed for extended theories of gravity. A simple interpretation of this instability as the effect of an "antifriction" is given, and it is argued that a universe must eventually enter the expanding phase.Comment: 4 pages, latex, to appear in Eur. Phys. J.

Interaction of Low - Energy Induced Gravity with Quantized Matter and Phase Transition Induced by Curvature

At high energy scale the only quantum effect of any asymptotic free and asymptotically conformal invariant GUT is the trace anomaly of the energy-momentum tensor. Anomaly generates the new degree of freedom, that is propagating conformal factor. At lower energies conformal factor starts to interact with scalar field because of the violation of conformal invariance. We estimate the effect of such an interaction and find the running of the nonminimal coupling from conformal value $\frac{1}{6}$ to $0$. Then we discuss the possibility of the first order phase transition induced by curvature in a region close to the stable fixed point and calculate the induced values of Newtonian and cosmological constants.Comment: 11 pages, LaTex, KEK-TH-397-KEK Preprint 94-3

Magnetized cosmological perturbations

A large-scale cosmic magnetic field affects not only the growth of density perturbations, but also rotational instabilities and anisotropic deformation in the density distribution. We give a fully relativistic treatment of all these effects, incorporating the magneto-curvature coupling that arises in a relativistic approach. We show that this coupling produces a small enhancement of the growing mode on superhorizon scales. The magnetic field generates new nonadiabatic constant and decaying modes, as well as nonadiabatic corrections to the standard growing and decaying modes. Magnetized isocurvature perturbations are purely decaying on superhorizon scales. On subhorizon scales before recombination, magnetized density perturbations propagate as magneto-sonic waves, leading to a small decrease in the spacing of acoustic peaks. Fluctuations in the field direction induce scale-dependent vorticity, and generate precession in the rotational vector. On small scales, magnetized density vortices propagate as Alfv\'{e}n waves during the radiation era. After recombination, they decay slower than non-magnetized vortices. Magnetic fluctuations are also an active source of anisotropic distortion in the density distribution. We derive the evolution equations for this distortion, and find a particular growing solution.Comment: Revised version, typos corrected, to appear in Phys. Rev.

Worldsheet Instantons and a Null String Limit of Born-Infeld Theory

For a superstring theory in four spacetime dimensions, we propose a modification of the Born-Infeld action that possesses a well-defined tensionless limit. We interpret this as describing the effective target space dynamics of null strings on a D3-brane. We argue that such a modification can be induced by nonperturbative contributions from instantons in the worldsheet sigma-model describing string propagation on the brane.Comment: 11 pages; Comments and references adde

Spiral Multi-component Structure in Pade - Approximant QCD

We present a graphical method of analyzing the infra-red fixed point structure of Pade approximant QCD. The analysis shows a spiral multi-component couplant structure as well as an infra-red attractor behavior of PQCD couplant for all flavors $0 \le N_{f} \le 16$.Comment: 78 pages, 4 tables, 44 graph

Next-to-leading order QCD corrections to spin-dependent hadron-pair photoproduction

We compute the next-to-leading order QCD corrections to the ``direct'' part of the spin-dependent cross section for hadron-pair photoproduction. The calculation is performed using largely analytical methods. We present a brief phenomenological study of our results focussing on the $K$-factors and scale dependence of the next-to-leading order cross sections. This process is relevant for the extraction of the gluon polarization in present and future spin-dependent lepton-nucleon scattering experiments.Comment: 9 pages, 2 eps figure

Longitudinal Polarization of Lambda and anti-Lambda Hyperons in Lepton-Nucleon Deep-Inelastic Scattering

We consider models for the spin transfers to $\Lambda$ and $\bar{\Lambda}$ hyperons produced in lepton-nucleon deep-inelastic scattering. We make predictions for longitudinal $\Lambda$ and $\bar{\Lambda}$ spin transfers for the COMPASS experiment and for HERA, and for the spin transfer to $\Lambda$ hyperons produced at JLAB. We demonstrate that accurate measurements of the spin transfers to $\Lambda$ and $\bar{\Lambda}$ hyperons with COMPASS kinematics have the potential to probe the intrinsic strangeness in the nucleon. We show that a measurement of $\bar{\Lambda}$ polarisation could provide a clean probe of the spin transfer from $\bar{s}$ quarks and provides a new possibility to measure the antistrange quark distribution function. COMPASS data in a domain of x that has not been studied previously will provide valuable extra information to fix models for the nucleon spin structure. The spin transfer to $\bar{\Lambda}$ hyperons, which could be measured by the COMPASS experiment, would provide a new tool to distinguish between the SU(6) and Burkardt-Jaffe (BJ) models for baryon spin structure. In the case of the HERA electron-proton collider experiments with longitudinally-polarised electrons, the separation between the target and current fragmentation mechanisms is more clear. It provides a complementary probe of the strange quark distribution and helps distinguish between the SU(6) and BJ models for the $\Lambda$ and $\bar{\Lambda}$ spin structure. Finally, we show that the spin transfer to $\Lambda$ hyperons measured in a JLAB experiment would be dominated by the spin transfer of the intrinsic polarised-strangeness in the remnant nucleon, providing an independent way to check our model predictions.Comment: minor changes after accepted to EPJ