User-Centric and Interactive – Approaches to Evaluating FID Portals Exemplified by the Subject Information Service FID Performing Arts

Der vorliegende Artikel beschäftigt sich mit der Frage, welche Methoden genutzt werden können, um eine Evaluierung von Services und Angeboten von Fachinformationsdiensten nutzer*innenzentriert und interaktiv umzusetzen. Als Beispiel dient der Fachinformationsdienst Darstellende Kunst, bereitgestellt von der Universitätsbibliothek Johann Christian Senckenberg in Frankfurt am Main. Drei unterschiedliche Methoden werden in diesem Zusammenhang näher vorgestellt und ihre Anwendbarkeit für die Evaluierung von FID-Portalen oder vergleichbaren Rechercheportalen reflektiert: Leitfaden-Interviews mit Think-Aloud-Elementen, virtuelle Fokusgruppen sowie ein digitaler Card-Sorting-Ansatz.In this article, we scrutinize methods to evaluate the services and offerings of specialized information services in user-centric and interactive ways. The subject information service (FID) Performing Arts provided by the University Library “Johann Christian Senckenberg” in Frankfurt am Main, is used as an example. Three different approaches are presented in an analysis of their applicability and practicability for the evaluation of FID and research portals, including guided interviews involving think-aloud elements, virtual focus groups, and the digital card-sorting approach.Peer Reviewe

Spatial and Temporal Profiles of Growth Factor Expression during CNS Demyelination Reveal the Dynamics of Repair Priming

Demyelination is the cause of disability in various neurological disorders. It is therefore crucial to understand the molecular regulation of oligodendrocytes, the myelin forming cells in the CNS. Growth factors are known to be essential for the development and maintenance of oligodendrocytes and are involved in the regulation of glial responses in various pathological conditions. We employed the well established murine cuprizone model of toxic demyelination to analyze the expression of 13 growth factors in the CNS during de- and remyelination. The temporal mRNA expression profile during demyelination and the subsequent remyelination were analyzed separately in the corpus callosum and cerebral cortex using laser microdissection and real-time PCR techniques. During demyelination a similar pattern of growth factor mRNA expression was observed in both areas with a strong up-regulation of NRG1 and GDNF and a slight increase of CNTF in the first week of cuprizone treatment. HGF, FGF-2, LIF, IGF-I, and TGF-ß1 were up-regulated mainly during peak demyelination. In contrast, during remyelination there were regional differences in growth factor mRNA expression levels. GDNF, CNTF, HGF, FGF-2, and BDNF were elevated in the corpus callosum but not in the cortex, suggesting tissue differences in the molecular regulation of remyelination in the white and grey matter. To clarify the cellular source we isolated microglia from the cuprizone lesions. GDNF, IGF-1, and FGF mRNA were detected in the microglial fraction with a temporal pattern corresponding to that from whole tissue PCR. In addition, immunohistochemical analysis revealed IGF-1 protein expression also in the reactive astrocytes. CNTF was located in astrocytes. This study identified seven different temporal expression patterns for growth factors in white and grey matter and demonstrated the importance of early tissue priming and exact orchestration of different steps during callosal and cortical de- and remyelination

Efficacy and cost-effectiveness of two online interventions for children and adolescents at risk for depression (E.motion trial): study protocol for a randomized controlled trial within the ProHEAD consortium

Background: Depression is a serious mental health problem and is common in children and adolescents. Online interventions are promising in overcoming the widespread undertreatment of depression and in improving the help-seeking behavior in children and adolescents. Methods: The multicentre, randomized controlled E.motion trial is part of the German ProHEAD consortium (Promoting Help-seeking using E-technology for ADolescents). The objective of the trial is to investigate the efficacy and cost-effectiveness of two online interventions to reduce depressive symptomatology in high-risk children and adolescents with subsyndromal symptoms of depression in comparison to an active control group. Participants will be randomized to one of three conditions: (1) Intervention 1, a clinician-guided self-management program (iFightDepression®); (2) Intervention 2, a clinician-guided group chat intervention; and (3) Control intervention, a psycho-educational website on depressive symptoms. Interventions last six weeks. In total, N = 363 children and adolescents aged ≥ 12 years with Patient Health Questionnaire-9 modified for Adolescents (PHQ-A) scores in the range of 5–9 will be recruited at five study sites across Germany. Online questionnaires will be administered before onset of the intervention, at the end of the intervention, and at the six-month follow-up. Further, children and adolescents will participate in the baseline screening and the one- and two-year school-based follow-up assessments integrated in the ProHEAD consortium. The primary endpoint is depression symptomatology at the end of intervention as measured by the PHQ-A score. Secondary outcomes include depression symptomatology at all follow-ups, help-seeking attitudes, and actual face-to-face help-seeking, adherence to and satisfaction with the interventions, depression stigma, and utilization and cost of interventions. Discussion: This study represents the first randomized controlled trial (RCT) investigating efficacy and cost-effectiveness of two online interventions in children and adolescents aged ≥ 12 years at risk for depression. It aims to provide a better understanding of the help-seeking behavior of children and adolescents, potential benefits of E-mental health interventions for this age group, and new insights into so far understudied aspects of E-mental health programs, such as potential negative effects of online interventions. This knowledge will be used to tailor and improve future help offers and programs for children and adolescents and ways of treatment allocation. Trial registration: German Register for Clinical Trials (DRKS), DRKS00014668. Registered on 4 May 2018. International trial registration took place through the “international clinical trials registry platform” with the secondary ID S-086/2018

Ergebnisbericht zur qualitativen Evaluierung des Portals des Fachinformationsdienstes Darstellende Kunst

Ziel der im vorliegenden Ergebnisbericht vorgestellten Untersuchung war die Befragung der Nutzerinnen und Nutzer des Fachinformationsdienstes Darstellende Kunst (FID DK) der Universitätsbibliothek Johann Christian Senckenberg hinsichtlich Services, Inhalten und Usability des Online-Portals

Short-term fasting in glioma patients: analysis of diet diaries and metabolic parameters of the ERGO2 trial

Purpose: The prospective, randomized ERGO2 trial investigated the effect of calorie-restricted ketogenic diet and intermittent fasting (KD-IF) on re-irradiation for recurrent brain tumors. The study did not meet its primary endpoint of improved progression-free survival in comparison to standard diet (SD). We here report the results of the quality of life/neurocognition and a detailed analysis of the diet diaries. Methods: 50 patients were randomized 1:1 to re-irradiation combined with either SD or KD-IF. The KD-IF schedule included 3 days of ketogenic diet (KD: 21–23 kcal/kg/d, carbohydrate intake limited to 50 g/d), followed by 3 days of fasting and again 3 days of KD. Follow-up included examination of cognition, quality of life and serum samples. Results: The 20 patients who completed KD-IF met the prespecified goals for calorie and carbohydrate restriction. Substantial decreases in leptin and insulin and an increase in uric acid were observed. The SD group, of note, had a lower calorie intake than expected (21 kcal/kg/d instead of 30 kcal/kg/d). Neither quality of life nor cognition were affected by the diet. Low glucose emerged as a significant prognostic parameter in a best responder analysis. Conclusion: The strict caloric goals of the ERGO2 trial were tolerated well by patients with recurrent brain cancer. The short diet schedule led to significant metabolic changes with low glucose emerging as a candidate marker of better prognosis. The unexpected lower calorie intake of the control group complicates the interpretation of the results. Clinicaltrials.gov number: NCT01754350; Registration: 21.12.2012

Prediction of Response to Cisplatin-Based Neoadjuvant Chemotherapy of Muscle-Invasive Bladder Cancer Patients by Molecular Subtyping including KRT and FGFR Target Gene Assessment

Patients with muscle-invasive urothelial carcinoma achieving pathological complete response (pCR) upon neoadjuvant chemotherapy (NAC) have improved prognosis. Molecular subtypes of bladder cancer differ markedly regarding sensitivity to cisplatin-based chemotherapy and harbor FGFR treatment targets to various content. The objective of the present study was to evaluate whether preoperative assessment of molecular subtype as well as FGFR target gene expression is predictive for therapeutic outcome—rate of ypT0 status—to justify subsequent prospective validation within the “BladderBRIDGister”. Formalin-fixed paraffin-embedded (FFPE) tissue specimens from transurethral bladder tumor resections (TUR) prior to neoadjuvant chemotherapy and corresponding radical cystectomy samples after chemotherapy of 36 patients were retrospectively collected. RNA from FFPE tissues were extracted by commercial kits, Relative gene expression of subtyping markers (e.g., KRT5, KRT20) and target genes (FGFR1, FGFR3) was analyzed by standardized RT-qPCR systems (STRATIFYER Molecular Pathology GmbH, Cologne). Spearman correlation, Kruskal–Wallis, Mann–Whitney and sensitivity/specificity tests were performed by JMP 9.0.0 (SAS software). The neoadjuvant cohort consisted of 36 patients (median age: 69, male 83% vs. female 17%) with 92% of patients being node-negative during radical cystectomy after 1 to 4 cycles of NAC. When comparing pretreatment with post-treatment samples, the median expression of KRT20 dropped most significantly from DCT 37.38 to 30.65, which compares with a 128-fold decrease. The reduction in gene expression was modest for other luminal marker genes (GATA3 6.8-fold, ERBB2 6.3-fold). In contrast, FGFR1 mRNA expression increased from 33.28 to 35.88 (~6.8-fold increase). Spearman correlation revealed positive association of pretreatment KRT20 mRNA levels with achieving pCR (r = 0.3072: p = 0.0684), whereas pretreatment FGFR1 mRNA was associated with resistance to chemotherapy (r = −0.6418: p < 0.0001). Hierarchical clustering identified luminal tumors of high KRT20 mRNA expression being associated with high pCR rate (10/16; 63%), while the double-negative subgroup with high FGFR1 expression did not respond with pCR (0/9; 0%). Molecular subtyping distinguishes patients with high probability of response from tumors as resistant to neoadjuvant chemotherapy. Targeting FGFR1 in less-differentiated bladder cancer subgroups may sensitize tumors for adopted treatments or subsequent chemotherapy

The 50 gTSS of protein-coding genes associated with the highest number of reads.

<p>For each gTSS, the position with respect to the forward strand, the orientation (S), the number of reads, the gene ID, gene name (if known) and gene annotation is given. The gTSS are ordered according to the number of reads.</p

Analysis of loci encoding proteins of the CP43/IsiA/Pcb family.

<p><b>A.</b> Organization of the chromosomal region harboring the <i>isiA</i> and <i>psbC</i>-like genes (<i>psbC-lk1-3</i>) of <i>N. spumigena</i> and the separate <i>psbDC</i> operon. The PsaL–coding domain in <i>psbC-lk2</i> (<i>nsp37500</i>) is highlighted in orange. <b>B.</b> Phylogenetic analysis of CP43, IsiA and related chlorophyll-binding proteins from <i>N. spumigena</i> and of selected other cyanobacteria was inferred using the Minimum Evolution method. The optimal tree with the sum of branch length = 3,97009738 is shown. The percentage of replicate trees in which the associated taxa clustered in the bootstrap test (1000 replicates) are shown next to the branches. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Poisson correction method and are in the units of the number of amino acid substitutions per site. All positions containing gaps and missing data were eliminated from the dataset (complete deletion option). There were a total of 279 positions in the final dataset. <b>C.</b> Transcriptional organization around the <i>isiA, isiB</i> and <i>psbC</i>-like gene cluster. There are three mapped TSS in the region displayed in <b>Fig. 3A</b>, all associated with or close to the 5′ end of <i>nsp37510.</i> TSS are indicated by blue arrows and the number of cDNA reads associated with them are given as approximation for their activity. One gTSS gives rise to the 83 nt long 5′ UTR upstream of <i>nsp37510</i> (blue) and the gene or operon mRNA. An antisense RNA originates from a single aTSS in the opposite direction (purple). The third TSS is a putative nTSS driving the transcription of an ncRNA in the <i>nsp37510</i>- <i>nsp37520</i> intergenic spacer. Except for the <i>nsp37510</i> 5′ UTR, all TSS displayed are drawn with a 100 nt-long box that corresponded to the maximum read length in the dRNAseq approach.</p

General features of <i>N. spumigena</i> CCY9414.

<p><b>A</b>. Photomicrograph of <i>N. spumigena</i> CCY9414 trichomes. The arrows point to heterocysts. The vertical bar corresponds to 40 µm. <b>B</b>. Phylogenetic position of <i>N. spumigena</i> CCY9414 (boxed) within the cyanobacterial phyum, based on its two 16S rRNA sequences (labeled a and b). The two sub-clades within the Nostocales, clade I and clade II, are indicated. Species for which a total genome sequence is publicly available, are in blue. The sequence of <i>Chlorobium tepidum</i> TLS served as outgroup. The numbers at nodes refer to bootstrap support values (1000 repetitions) if >60%. The phylogenetic tree was generated using the Minimum Evolution method within MEGA5 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060224#pone.0060224-Tamura1" target="_blank">[158]</a>. The optimal tree with the sum of branch length  =  0.85445647 is shown. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree and are given in the number of base substitutions per site. The multiple sequence alignment was shortened to a total of 1407 positions in the final dataset to include also 16S rRNA sequences from species without a genome sequence.</p