Istraživanje medija različitog sadržaja na organiziranje meristema in vitro

Optimal conditions for in vitro multiplication and rooting of cv. Willamette were investigated. High level of proliferation and multiplication after 90 days of culture on Murashige and Skoog (MS) medium with 100 mg·l-1 myo-inositol, 2 mg·l-1 thiamine-HCl, 1 mg·l-1 piridoxine-HCl, 1 mg·l-1 nicotinic acid and 30 g·l-1 sucrose and 8 g·l-1 agar was considered together with two concentrations of BA (0,2 and 2 mg·l-1). Half strength MS medium was also used. There was a significant variation of the proliferated shoots, depending on media. We got high level of rooting and we have investigated acclimatization of regenerants grown on sterile substrate in conditions of high humidity.Istraživani su optimalni uvjeti za razmanžanje i ukorjenjivanje kultivara Willamette in vitro. Visok stupanj proliferacije i razmanžanja nakon 90 dana kulture na Murashige i Skoog (MS) mediju sa 100 mg·l-1 mio inositola, 2 mg·l-1 tijamina-HCl, 1 mg·l-1 piridoksina-HCl, 1 mg·l-1 nikotinske kiseline, 30 g·l-1 saharoze i 8 g·l-1 agara razmatran je s dvije koncentracije BA (0,2 i 2,0 mg·l-1). Primijenjen je i medij MS pola koncentracije. Značajno je bilo odstupanje proliferiranih izboja, ovisno o mediju. Dobili smo visoku razinu ukorjenjenja te smo istražili prilagodbu ponovno naraslih izboja na sterilnom supstratu u uvjetima obilne vlage

Agro ecological conditions for viticulture development in Gevgelija region

In this publication detail climate analysis of air temperature, length of sun glow and rain quantities has been made for the Gevgelija-Valandovo region for period 1961/1990, 1991/2000 and 2001/2009. Climate-viticulture bulletin has been made for viticulture requirement. Climate index and coefficients have been determined in the examined area. Only careful and right choice of viticulture and climate analyses can contribute to result in quality and quantity manufacturing of viticulture

Istraživanje medija različitog sadržaja na organiziranje meristema in vitro

Optimal conditions for in vitro multiplication and rooting of cv. Willamette were investigated. High level of proliferation and multiplication after 90 days of culture on Murashige and Skoog (MS) medium with 100 mg·l-1 myo-inositol, 2 mg·l-1 thiamine-HCl, 1 mg·l-1 piridoxine-HCl, 1 mg·l-1 nicotinic acid and 30 g·l-1 sucrose and 8 g·l-1 agar was considered together with two concentrations of BA (0,2 and 2 mg·l-1). Half strength MS medium was also used. There was a significant variation of the proliferated shoots, depending on media. We got high level of rooting and we have investigated acclimatization of regenerants grown on sterile substrate in conditions of high humidity.Istraživani su optimalni uvjeti za razmanžanje i ukorjenjivanje kultivara Willamette in vitro. Visok stupanj proliferacije i razmanžanja nakon 90 dana kulture na Murashige i Skoog (MS) mediju sa 100 mg·l-1 mio inositola, 2 mg·l-1 tijamina-HCl, 1 mg·l-1 piridoksina-HCl, 1 mg·l-1 nikotinske kiseline, 30 g·l-1 saharoze i 8 g·l-1 agara razmatran je s dvije koncentracije BA (0,2 i 2,0 mg·l-1). Primijenjen je i medij MS pola koncentracije. Značajno je bilo odstupanje proliferiranih izboja, ovisno o mediju. Dobili smo visoku razinu ukorjenjenja te smo istražili prilagodbu ponovno naraslih izboja na sterilnom supstratu u uvjetima obilne vlage

The effect of pruning and cluster thinning for producing of dessert wine's raw material from semillon cultivar in Skopje wine district's conditions

Possibilities of producing dessert wine's raw material from Semillon cultivar has been researched in the Skopje vineyard district climate condition. The following treatments were applied: pruning (16, 20 and 24 buds/vine) and thinning of the cluster (without thinning, 1 cluster/shoot and 1 cluster/2 shoot). In two moments of ripening (full ripe and 20 days after full ripe stage) the yield and content of sugar in must has been researched. The yield and content of sugar in must statistically differ. Depending of the treatment raw material for different types of dessert wine has been obtained 185-233 g/dm3 sugar in full ripe and 227-271 g/dm3 after full ripe stage. Value of content of sugar is obtained from must of all grapes from one vine. Highest value of content og sugar is possible to obtain with picking of raisin grape

Investigations of anatomical characteristics of some wine and table - grapevine cultivars, in Skopje area of vineyards

Comparative researches of anatomical characteristics on the plant (grapevine cultivars Vitis Vinifera) are important for disposition in the area around oneself(training system). Anatomical and physiogical charateristics are genetic determinate and they indicate of the genotypical variability. In this paper are presented results from investigations of some anatomical and physiological characteristics on mature branches of some cultivars (dattier, muscat hamburg, vranec, merlot, zilavka and riezling). Anatomical characteristics are the internode length, anatomical construction of buds, a diameter etc., and they are features of cultivars. The researches were conducted during the period 2002-2005 in the grapevine plantations of the Institute of Agriculture, Skopje. The mature branches, in two variants were measured - from 1 to 8 bud, and from 1 to 10 bud. The internodes, from 4 to 10 bud, separately were measured. The anatomical construction of buds and diameter were observed with diagonal and longitudinal cuts. They were photographed, separately. Fom analysis, mean value from 30 representative samples was taken. For comparison of the analysed characteristics at investigated cultivars, the official O.I.V botanical classification (description) was used. The obtained result of the analysed parametars are of a great importance for physiological condition of plant, approprition of training system, trunk number per unit of surface, planting distance, fertility of buds an so on

Microsatellite marker analysis of macedonian grapevines (<em>Vitis vinifera</em> L.) compared to Bulgarian and Greek cultivars

Aims: Eleven Macedonian grapevine accessions were genotyped by microsatellite profiling at 9 microsatellite loci, in order to identify Macedonian cultivars and to evaluate the relationships among them. The comparison with grapevine cultivars from two neighbouring countries was also performed. Methods and results: Clustering analyses based on the proportion of shared alleles resulted in two clusters containing all accessions except cultivar « Vranec », which was distant from the others. Comparison of genotyping results of Macedonian accessions with 76 Bulgarian and 298 Greek accessions revealed no identical genotypes. In the dendogram, Macedonian accessions are dispersed among Greek and Bulgarian grapevines, suggesting a common genetic background. Additionaly, the synonyms « Smederevka » = « Dimyat » = « Zoumiatiko » and « Belo Zimsko » = « Karatsova Naousis » were also evaluated. Conclusions: Clustering analyses showed that authentic Macedonian cultivars are distant from two widespread cultivars « Vranec » and « Smederevka ». Comparison of Macedonian cultivars with their synonyms from Greece and Bulgaria revealed differences in allelic profiles at some loci, but further analyses are needed to confirm their unique allelic profiles. Significance and impact of study: This work is a first step towards the genetic characterization of Macedonian grapevine germplasm, thus contributing to the molecular investigation of grapevine germplasm within the Balkan region