A designer hyper interleukin 11 (H11) is a biologically active cytokine

<p>Abstract</p> <p>Background</p> <p>Interleukin 11 (IL-11) is a pleiotropic cytokine with anti-apoptotic, anti-inflammatory and hematopoietic potential. The IL-11 activity is determined by the expression of the IL-11R receptor alpha (IL-11Rα) and the signal transducing subunit β (gp130) on the cell membrane. A recombinant soluble form of the IL-11Rα (sIL-11Rα) in combination with IL-11 acts as an agonist on cells expressing the gp130 molecule. We constructed a designer cytokine Hyper IL-11 (H11), which is exclusively composed of naturally existing components. It contains the full length sIL-11Rα connected with the mature IL-11 protein using their natural sequences only. Such a construct has two major advantages: (i) its components are as close as possible to the natural forms of both proteins and (ii) it lacks an artificial linker what should avoid induction of antibody production.</p> <p>Results</p> <p>The H11 construct was generated, the protein was produced in a baculovirus expression system and was then purified by using ion exchange chromatography. The H11 protein displayed activity in three independent bioassays, (i) it induced acute phase proteins production in HepG2 cells expressing IL-11, IL-11Rα and gp130, (ii) it stimulated the proliferation of B9 cells (cells expressing IL-11Rα and gp130) and (iii) proliferation of Baf/3-gp130 cells (cells not expressing IL-11 and IL-11Rα but gp130). Moreover, the preliminary data indicated that H11 was functionally distinct from Hyper-IL-6, a molecule which utilizes the same homodimer of signal transducing receptor (gp130).</p> <p>Conclusions</p> <p>The biologically active H11 may be potentially useful for treatment of thrombocytopenia, infertility, multiple sclerosis, cardiovascular diseases or inflammatory disorders.</p

Quality of life of the oncological patients, undergoing the chemotherapy

This bachelor's thesis discuss the individual perception of the qualitiy of life of the oncological patiens. It also describes the problems of livng with cancer, characterizes the palliative care and focuses on nursing care in oncology, as well as it determines the concepts - quality of life and posttraumatic growth. The main focus lies in the qulitative research, which compres the differences in perception of the quality of life between two goups of patients. First, a group of patients, currently undergoing chemotherapy, and second, a group of patients with already finished treatment and recently in remision of their oncological disease. The interviews with patients help to describe the influence of cancer on quality of their life, what good or which lesson it had brought to their lives. The other aim was to determine the "crisis level" by the "crisis curve graph"

The effect of H11 on the intensity of the fibrosis of spleen compared with the age-matched naïve mice (control), vehicle- (PBS) and IL-11-treated mice after a single irradiation.

<p>The effect of H11 on the intensity of the fibrosis of spleen compared with the age-matched naïve mice (control), vehicle- (PBS) and IL-11-treated mice after a single irradiation.</p

The effect of H11 on the numbers of spleen BFU-E, CFU-GM, CFU-GEMM and CFU-Mk compared with the age-matched naïve mice (control), vehicle- (PBS) and IL-11-treated mice after radiotherapy administration.

<p>The effect of H11 on the numbers of spleen BFU-E, CFU-GM, CFU-GEMM and CFU-Mk compared with the age-matched naïve mice (control), vehicle- (PBS) and IL-11-treated mice after radiotherapy administration.</p

The effect of H11 on the numbers of spleen BFU-E, CFU-GM, CFU-GEMM and CFU-Mk compared with the age-matched naïve mice (control), vehicle- (PBS) and IL-11-treated mice after chemo/radiotherapy.

<p>The effect of H11 on the numbers of spleen BFU-E, CFU-GM, CFU-GEMM and CFU-Mk compared with the age-matched naïve mice (control), vehicle- (PBS) and IL-11-treated mice after chemo/radiotherapy.</p

Effects of Designer Hyper-Interleukin 11 (H11) on Hematopoiesis in Myelosuppressed Mice - Fig 3

<p><b>Effect of H11 on the number of bone marrow and spleen cells after (B) chemo/radiotherapy or (C) radiotherapy induced myelosuppression compared with vehicle (PBS), IL-11-treated mice and (A) age-matched naïve mice (control).</b> The bone marrow and spleen cells were pooled from 5 animals per each time point, and the data are shown as the mean number of cells per animal, except for the results indicated by a # sign, which represents the average data from two animals.</p

Levels of alanine transaminase (ALT), aspartate transaminase (AST) and lactate dehydrogenase (LDH) in the serum of naïve (control) mice and mice after a single irradiation and treatment with PBS, IL-11 and H11.

<p>Levels of alanine transaminase (ALT), aspartate transaminase (AST) and lactate dehydrogenase (LDH) in the serum of naïve (control) mice and mice after a single irradiation and treatment with PBS, IL-11 and H11.</p

The effect of H11 on the numbers of bone marrow BFU-E, CFU-GM, CFU-GEMM and CFU-Mk compared with the age-matched naïve mice (control), vehicle- (PBS) and IL-11-treated mice after radiotherapy administration.

<p>The effect of H11 on the numbers of bone marrow BFU-E, CFU-GM, CFU-GEMM and CFU-Mk compared with the age-matched naïve mice (control), vehicle- (PBS) and IL-11-treated mice after radiotherapy administration.</p