Characteristics of 163 patients with <i>Candida parapsilosis</i> complex candidemia.

<p>Characteristics of 163 patients with <i>Candida parapsilosis</i> complex candidemia.</p

MICs and EAs of the E-test and Sensititre methods compared with those of the CLSI and EUCAST methods.

<p>MICs and EAs of the E-test and Sensititre methods compared with those of the CLSI and EUCAST methods.</p

Microbiota in anorexia nervosa: The triangle between bacterial species, metabolites and psychological tests

<div><p>Anorexia nervosa (AN) is a psychiatric disease with devastating physical consequences, with a pathophysiological mechanism still to be elucidated. Metagenomic studies on anorexia nervosa have revealed profound gut microbiome perturbations as a possible environmental factor involved in the disease. In this study we performed a comprehensive analysis integrating data on gut microbiota with clinical, anthropometric and psychological traits to gain new insight in the pathophysiology of AN. Fifteen AN women were compared with fifteen age-, sex- and ethnicity-matched healthy controls. AN diet was characterized by a significant lower energy intake, but macronutrient analysis highlighted a restriction only in fats and carbohydrates consumption. Next generation sequencing showed that AN intestinal microbiota was significantly affected at every taxonomic level, showing a significant increase of <i>Enterobacteriaceae</i>, and of the archeon <i>Methanobrevibacter smithii</i> compared with healthy controls. On the contrary, the genera <i>Roseburia</i>, <i>Ruminococcus</i> and <i>Clostridium</i>, were depleted, in line with the observed reduction in AN of total short chain fatty acids, butyrate, and propionate. Butyrate concentrations inversely correlated with anxiety levels, whereas propionate directly correlated with insulin levels and with the relative abundance of <i>Roseburia inulinivorans</i>, a known propionate producer. BMI represented the best predictive value for gut dysbiosis and metabolic alterations, showing a negative correlation with <i>Bacteroides uniformis</i> (microbiota), with alanine aminotransferase (liver function), and with psychopathological scores (obsession-compulsion, anxiety, and depression), and a positive correlation with white blood cells count. In conclusion, our findings corroborate the hypothesis that the gut dysbiosis could take part in the AN neurobiology, in particular in sustaining the persistence of alterations that eventually result in relapses after renourishment and psychological therapy, but causality still needs to be proven.</p></div

Taxa significantly increased (+) or decreased (-) in abundance in anorexia nervosa subjects.

<p>Taxa significantly increased (+) or decreased (-) in abundance in anorexia nervosa subjects.</p

Raw scores for psychopathological assessment.

<p>Raw scores for psychopathological assessment.</p

Distance-based redundancy analysis (db-RDA).

<p>Db-RDA plot shows correlations between gut microbiota composition and body mass index (BMI), insulin, propionate and butyrate. Only significant variables are represented (p>0.05, Permanova analysis). Arrows in the db-RDA biplot denote the magnitudes and directions of the variable effects. Controls are represented by blue dots and anorexic subjects by red dots.</p

Fecal SCFA concentrations in anorexia nervosa subjects and healthy control group.

<p>Fecal SCFA concentrations in anorexia nervosa subjects and healthy control group.</p

Characteristics of the study population.

<p>Characteristics of the study population.</p

Redundancy analysis (RDA) of gut microbiota.

<p>RDA shows a separation between anorexia nervosa (AN, red dots) and control (CTR, blue dots) subjects.</p

Relative abundance of common microbial taxa.

<p>Bar charts showing the relative abundance of the most represented microbial taxa, defined as having a mean relative abundance of >1%, in stool samples of anorexia nervosa (AN, n = 15) and control (CTR, n = 15) groups. Phylum-level (<b>a</b>) and family-level (<b>b</b>) taxon profiles are shown.</p