Potassium elemental distribution of (parts of) single fibroblast cells obtained from control patient ‘LR’ and Friedreich’s ataxia patient ‘SL’.

<p>For each case, 5 different fibroblasts were scanned. Boundaries of the fibroblasts are indicated, as well as their nuclei (if present). Scans were obtained in ‘Low Dose’ mode, with a step size of 100 nm and a dwell time of 50 ms. Maps are normalized to incoming intensity, 1s measuring time and are dead time corrected.</p

Mean biomass-per-unit-effort (BPUE) of the eleven most commonly caught species from 2007–2013.

<p>BPUE is represented as the estimated weight (kg) of fishes caught-per-angler-hour, using two hooks per line. Solid lines illustrate catch rates from marine protected areas (MPA), whereas dashed lines represent values from associated reference (REF) sites. Error bars denote one standard error above and below the mean.</p

Potassium and iron elemental distribution within control fibroblast case ‘PN’ (upper row) and FRDA fibroblast case ‘DJS’ (lower row).

<p>Scale bar indicates the background-corrected mass fraction in ppm, calculated from a mean cell thickness of 10 μm. Pixel size in the images is 55 nm; acquisition time for each pixel is 50 ms. Elemental maps were measured in ‘High dose’ mode and based on detector no. 5 (XIA05) only. All element maps were normalized to dead time, ring current and quantified using the Fundamental Parameter method, taking into account the ice layer thickness determined using the K-Kα/Kβ ratio. Red striped circles indicate areas with iron hot-spots, circles h1-h3 indicate exogenous iron hot-spots, white arrows spherical structures and red arrows fibre-like structures in the fibroblast cells.</p

Background-corrected mean mass fractions of the elements P, S, Cl, K, Ca, Mn, Fe, Ni, Cu and Zn in the cytoplasm of 5 fibroblasts from control case ‘LR’ and FRDA case ‘SL’.

<p>Background-corrected mean mass fractions of the elements P, S, Cl, K, Ca, Mn, Fe, Ni, Cu and Zn in the cytoplasm of 5 fibroblasts from control case ‘LR’ and FRDA case ‘SL’.</p

Prussian blue staining and TEM on FRDA fibroblasts.

<p>a) Light microscopic image of healthy human fibroblasts; b) TEM image of human fibroblast; c) negative Prussian blue staining of human control fibroblasts; d) positive Prussian blue staining of fibroblasts from Friedreich’s ataxia (FRDA) patients. Iron-rich regions are clearly present in the FRDA fibroblast cells as bright blue regions (indicated with arrows), while fibroblast nuclei and cytoplasm have a red and pink color, respectively.</p

Background-corrected mean mass fractions of the elements P, S, Cl, K, Ca, Mn, Fe, Ni, Cu and Zn within 1) entire cell body 2) nucleus and 3) cytoplasm of a single fibroblast from control patient ‘PN’ and Friedreich’s ataxia patient ‘DJS’.

<p>Background-corrected mean mass fractions of the elements P, S, Cl, K, Ca, Mn, Fe, Ni, Cu and Zn within 1) entire cell body 2) nucleus and 3) cytoplasm of a single fibroblast from control patient ‘PN’ and Friedreich’s ataxia patient ‘DJS’.</p

Potassium elemental distribution of (parts of) single fibroblast cells obtained from control patient ‘LR’ and Friedreich’s ataxia patient ‘SL’.

<p>For each case, 5 different fibroblasts were scanned. Boundaries of the fibroblasts are indicated, as well as their nuclei (if present). Scans were obtained in ‘Low Dose’ mode, with a step size of 100 nm and a dwell time of 50 ms. Maps are normalized to incoming intensity, 1s measuring time and are dead time corrected.</p

Elemental distribution of P, S, Cl, Ca, Zn and Compton scatter within control fibroblast case ‘PN’ (upper row) and FRDA fibroblast case ‘DJS’ (lower row).

<p>Experimental conditions: see legend of <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0190495#pone.0190495.g002" target="_blank">Fig 2</a>. White dashed circle indicates the nucleus border. n1-2 indicate the presence of nucleoli.</p