APRENDIZADO DE MATEMÁTICA USANDO JOGOS NA EDUCAÇÃO ESPECIAL

Games are important for special education, because, when adapted to the teaching-learning process, they allow cultural content and scientific knowledge to be address in a playful and entertaining, way with challenges and overcoming. Our goal was to use games as pedagogical support to arouse interest in learning mathematics in special education students. This research was carried out in two different moments, with activities at a municipal school and tourist spots of the city of Manaus. Fourteen students with special needs (autism, cerebral palsy, and intellectual disability) from the 3rd phase of special education for young people and adults participated in this qualitative and descriptive research. Therefore, we selected, adapted, and reformulated math games according to the reality and needs of students. To that end, we used complementary techniques like rounds of conversation, didactic sequence, and guided visits. The pedagogical proposal provided a range of knowledge, stimulating their logical and mathematical thinking, by mean of simple calculations and broad development of cognitive, motor, and social affective skills, which contributed to the teaching-learning process. We noticed the educational potential of the use of math games with education spaces outside school. This means, the exploration of the tourist spots visited that enriched the students' learning in mathematics.Os jogos são importantes para estudantes de Educação Especial porque quando adaptados ao processo de ensino-aprendizagem permitem a assimilação de conteúdo cultural e conhecimento científico em uma interação promotora de ludicidade, entretenimento, desafios e superação. Este estudo tem por objetivo relacionar o uso dos jogos como suporte pedagógico a fim de despertar o interesse do aluno no conteúdo curricular de Matemática na educação especial. A pesquisa sucedeu em dois momentos: com atividades realizadas numa escola municipal da cidade de Manaus e em visitas a pontos turísticos da capital amazonense, tendo como sujeitos 14 alunos com necessidades especiais, sendo estas: autismo, paralisia cerebral e deficiência intelectual. Os indivíduos da pesquisa estão matriculados na 3ª fase de Educação Especial de jovens e adultos. Trata-se de um estudo com abordagem qualitativa e descritiva no qual verificamos a efetividade do uso de jogos de Matemática selecionados, adaptados e reformulados dentro da realidade e das necessidades dos alunos, com o apoio complementar das técnicas de roda de conversa, sequência didática e visita guiada. Pudemos auferir o êxito da proposta pedagógica com a demonstração da ampliação dos conhecimentos, do estímulo ao raciocínio lógico matemático por meio da realização de cálculos simples e ainda um amplo desenvolvimento de habilidades afetivas cognitivas, motoras e sociais, evidenciando contribuição para o processo de ensino-aprendizagem. Percebemos o potencial educacional do uso de jogos interrelacionados ao convívio com ambientes educativos fora do context

Molecular Characterization and Phylogenetic Study of Coxsackievirus A24v Causing Outbreaks of Acute Hemorrhagic Conjunctivitis (AHC) in Brazil

Coxsackievirus A24 variant (CA24v) is the most prevalent viral pathogen associated with acute hemorrhagic conjunctivitis (AHC) outbreaks. Sixteen years after its first outbreak in Brazil, this agent reemerged in 2003 in Brazil, spread to nearly all states and caused outbreaks until 2005. In 2009, a new outbreak occurred in the northeast region of the country. In this study, we performed a viral isolation in cell culture and characterized clinical samples collected from patients presenting symptoms during the outbreak of 2005 in Vitória, Espírito Santo State (ES) and the outbreak of 2009 in Recife, Pernambuco State (PE). We also performed a phylogenetic analysis of worldwide strains and all meaningful Brazilian isolates since 2003.Sterile cotton swabs were used to collect eye discharges, and all 210 clinical samples were used to inoculate cell cultures. Cytopathic effects in HEp-2 cells were seen in 58 of 180 (32%) samples from Vitória and 3 of 30 (10%) samples from Recife. Phylogenetic analysis based on a fragment of the VP1 and 3C gene revealed that the CA24v causing outbreaks in Brazil during the years 2003, 2004 and 2005 evolved from Asian isolates that had caused the South Korean outbreak of AHC during the summer of 2002. However, the 2009 outbreak of AHC in Pernambuco was originated from the reintroduction of a new CA24v strain that was circulating during 2007 in Asia, where CA24v outbreaks has been continuously reported since 1970.This study is the first phylogenetic analysis of AHC outbreaks caused by CA24v in Brazil. The results showed that Asian strains of CA24v were responsible for the outbreaks since 1987 and were independently introduced to Brazil in 2003 and 2009. Phylogenetic analysis of complete VP1 gene is a useful tool for studying the epidemiology of enteroviruses associated with outbreaks

Perfil genômico dos poliovírus de origem vacinal isolados de casos de paralisias flácidas agudas, no Brasil, no período pós-eliminação dos poliovírus selvagens da região das Américas

Submitted by Anderson Silva ([email protected]) on 2012-11-28T12:37:39Z No. of bitstreams: 1 eliane_v_costa_ioc_bcm_0043_2011.pdf: 4529718 bytes, checksum: fa63bf5abfb95e39c6504892dd9a4e9e (MD5)Made available in DSpace on 2012-11-28T12:37:39Z (GMT). No. of bitstreams: 1 eliane_v_costa_ioc_bcm_0043_2011.pdf: 4529718 bytes, checksum: fa63bf5abfb95e39c6504892dd9a4e9e (MD5) Previous issue date: 2011Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.A poliomielite (poliomielite anterior aguda, paralisia infantil) é uma doença infecciosa de caráter agudo que ocorre seguida a uma infecção causada por um dos três sorotipos de poliovírus, denominados poliovírus tipos 1, 2 e 3. Em 1988, quando os poliovírus selvagens eram endêmicos em 125 países ficou estabelecida durante a 41a Assembléia Mundial da Saúde, em Genebra, a meta da erradicação da poliomielite até o ano 2000. Os poliovírus selvagens estão hoje restritos a apenas quatro países (Nigéria, Afeganistão, Paquistão e Índia). Este grande sucesso é atribuído à utilização sistemática da vacina oral contra a poliomielite (VOP), desenvolvida pelo Dr. Albert Sabin. Esta vacina, licenciada nos anos de 1960s, e que vem sendo utilizada por mais de quatro décadas, consiste de variantes atenuadas de cada um dos três sorotipos de poliovírus. Por ser uma vacina constituída por vírus vivos atenuados, os problemas a ela associados estão principalmente ligados à sua instabilidade genética e a possibilidade do aparecimento de mutações e recombinações nas subpopulações virais excretadas. Durante a replicação do vírus vacinal em humanos, frequentemente ocorre reversão de algumas substituições nucleotídicas que conferem o fenótipo atenuado. Essa é a principal causa do aparecimento de raros casos de poliomielite associados à vacina (VAPP), relatados em diferentes países, assim como surtos de poliomielite paralítica devidos à infecção por estes vírus vacinais derivados (PVDV) que possuem propriedades biológicas semelhantes aos poliovírus selvagens. A análise e caracterização de quatro regiões distintas do genoma viral (5’NC, VP1, 2C e 3D), dos poliovírus vacinais isolados no Laboratório de Enterovírus do Instituto Oswaldo Cruz, a partir de amostras clínicas de casos de paralisia flácida aguda que ocorreram no Brasil, no período de 1995 a 2007, constituíram no principal objetivo do presente trabalho. Um total de 177 amostras dos três sorotipos foi analisado por sequenciamento nucleotídico em todo o gene que codifica a VP1; 222 amostras foram analisadas por duplex RT-PCR nas regiões 2C e 3D; 68 amostras foram analisadas na região 5´- NC e 44 amostras foram analisadas nas 4 regiões (5´NC, VP1, 2C e 3D). Dois isolados de PV2, apresentando mais de 1% de mutação em VP1, foram identificados e classificados como PVDV2. Existem aproximadamente 40 relatos de PVDV relacionados a pacientes imunodeficientes de 1962 a 2010, globalmente distribuídos, porém nenhum identificado no Brasil. Das 68 amostras sequenciadas na região 5’NC visando à avaliação de uma importante posição nucleotidica envolvida na atenuação da neurovirulência em cada um dos 3 sorotipos (PV1480, PV2481 e PV3472), os seguintes resultados foram encontrados: PV1=18.5% mutadas na posição 480, PV2=65% mutadas em 481 e PV3=81% mutadas em 472. Recombinação (em 2C/3D) foi encontrada em 15 amostras: 1 PV1 (1,3%), 5 PV2 (6,6%) e 9 PV3 (13,2%). Os três sorotipos de poliovírus apresentaram comportamento distinto em relação às análises realizadas, sendo o sorotipo 3 o menos estável em relação ao padrão de recombinação e reversão à neurovirulência seguido pelo sorotipo 2 e o sorotipo 1 foi o mais estável. A monitorização do perfil genômico dos poliovírus vacinais em circulação é essencial no estágio final de erradicação global da poliomielite.Poliomyelitis (acute anterior poliomyelitis, infantile paralysis) is an acute infectious disease which occurs subsequent to an infection caused by any of the three serotypes of polioviruses, designated poliovirus types 1, 2 and 3. In 1988, when wild polioviruses were endemic in 125 countries, the 41st World Health Assembly in Geneva set a goal for poliomyelitis eradication by the year 2000. Nowadays, poliomyelitis in its endemic form is restricted to only four countries: Nigeria, Afghanistan, Pakistan, and India. This remarkable success is attributed to the systematic use of oral poliomyelitis vaccine (OPV), developed by Dr. Albert Sabin. This vaccine, which was licensed in the 1960´s and is in use for over four decades, consists of attenuated variants of each of the three poliovirus serotypes. Because OPV consists of live attenuated viruses, the problems associated with this vaccine are mainly related to its genetic instability, which favor the emergence of mutations and recombinations among the excreted viral sub-populations. Also, during the process of virus replication in humans, some nucleotide substitutions responsible for the attenuated phenotype are often reverted. That is the main cause of the appearance of rare cases of vaccine-associated paralytic poliomyelitis (VAPP) reported in several countries as well as paralytic poliomyelitis outbreaks caused by vaccine-derived polioviruses which exhibit biological properties similar to wild polioviruses. The genomic characterization in four distinct regions of the genome (5’NC, VP1, 2C, and 3D) of vaccine-related polioviruses isolated in our laboratory from clinical samples of acute flaccid paralysis cases which occurred in Brazil, from 1995 to 2007 was the main purpose of the present work. A total of 177 isolates of the three serotypes of polioviruses were analyzed by nucleotide sequencing on the entire gene that codifies VP1; 222 isolates were analyzed by duplex PCR in 2C and 3D regions; 68 isolates were analyzed at the 5´- NCR while 44 isolates were analyzed in the four regions (5´- NCR, VP1, 2C and 3D)., Two PV2 isolates presenting over 1% of mutations in VP1 were identified and classified as VDPV2. There are approximately 40 reports of VDPV related to immunodefficient patients around the world from 1962 to 2010, but none of those occurred in Brazil. From the 68 isolates which were analyzed on the 5’NC region, in order to evaluate an important nucleotide position involved in attenuation of neurovirulence in each of the 3 serotypes (PV1480, PV2481 e PV3472). The following results were found: PV1 = 18.5% mutated at position 480, PV2 = 65% mutated at position 481 and PV3 = 81% mutated at 472. Recombination was found in 15 isolates: 1 PV1 (1.3%), 5 PV2 (6.6%) e 9 PV3 (13.2%). The three serotypes of polioviruses behavior distinctly to the analyses: type 3 showed to be the the least stable regarding the recombination and neurovirulence reversion patterns followed by serotype 2 while serotype 1 was the most stable. The knowledge regarding the genomic pattern of circulating polioviruses is essential at the final stages of poliomyelitis eradicatio

APRENDIZADO DE MATEMÁTICA USANDO JOGOS NA EDUCAÇÃO ESPECIAL

Games are important for special education, because, when adapted to the teaching-learning process, they allow cultural content and scientific knowledge to be address in a playful and entertaining, way with challenges and overcoming. Our goal was to use games as pedagogical support to arouse interest in learning mathematics in special education students. This research was carried out in two different moments, with activities at a municipal school and tourist spots of the city of Manaus. Fourteen students with special needs (autism, cerebral palsy, and intellectual disability) from the 3rd phase of special education for young people and adults participated in this qualitative and descriptive research. Therefore, we selected, adapted, and reformulated math games according to the reality and needs of students. To that end, we used complementary techniques like rounds of conversation, didactic sequence, and guided visits. The pedagogical proposal provided a range of knowledge, stimulating their logical and mathematical thinking, by mean of simple calculations and broad development of cognitive, motor, and social affective skills, which contributed to the teaching-learning process. We noticed the educational potential of the use of math games with education spaces outside school. This means, the exploration of the tourist spots visited that enriched the students' learning in mathematics.Os jogos são importantes para estudantes de Educação Especial porque quando adaptados ao processo de ensino-aprendizagem permitem a assimilação de conteúdo cultural e conhecimento científico em uma interação promotora de ludicidade, entretenimento, desafios e superação. Este estudo tem por objetivo relacionar o uso dos jogos como suporte pedagógico a fim de despertar o interesse do aluno no conteúdo curricular de Matemática na educação especial. A pesquisa sucedeu em dois momentos: com atividades realizadas numa escola municipal da cidade de Manaus e em visitas a pontos turísticos da capital amazonense, tendo como sujeitos 14 alunos com necessidades especiais, sendo estas: autismo, paralisia cerebral e deficiência intelectual. Os indivíduos da pesquisa estão matriculados na 3ª fase de Educação Especial de jovens e adultos. Trata-se de um estudo com abordagem qualitativa e descritiva no qual verificamos a efetividade do uso de jogos de Matemática selecionados, adaptados e reformulados dentro da realidade e das necessidades dos alunos, com o apoio complementar das técnicas de roda de conversa, sequência didática e visita guiada. Pudemos auferir o êxito da proposta pedagógica com a demonstração da ampliação dos conhecimentos, do estímulo ao raciocínio lógico matemático por meio da realização de cálculos simples e ainda um amplo desenvolvimento de habilidades afetivas cognitivas, motoras e sociais, evidenciando contribuição para o processo de ensino-aprendizagem. Percebemos o potencial educacional do uso de jogos interrelacionados ao convívio com ambientes educativos fora do context

Biochemical, hormonal and genetic evaluation of the families of two Brazilian patients with type 2 familial partial lipodystrophy (Partially pictured article)

ARTIGO PARCIALMENTE RETRATADO - O Conselho Editorial dos Arquivos Brasileiros de Endocrinologia e Metabologia, ISSN 0004 2730, decidiu, após análise, retratar as figuras 1 e 2 do seguinte artigo original: Caldas D, Silva Júnior WS, Simonetti JP, Costa EV, de Farias MLF. Avaliação bioquímica, hormonal e genética das famílias de duas pacientes brasileiras portadoras de lipodistrofia parcial familiar tipo 2. ABEM. 2013;57/8:583-93. Trata-se de solicitação das pacientes retratadas. Lamentamos qualquer mal-entendido causado ao nosso leitor.Submitted by Sandra Infurna ([email protected]) on 2019-12-03T15:03:01Z No. of bitstreams: 1 JosePascoaSimonetti_etal_IOC_2013.pdf: 550641 bytes, checksum: bc89614583d69ebc4f261c9139d11e83 (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2019-12-03T15:59:21Z (GMT) No. of bitstreams: 1 JosePascoaSimonetti_etal_IOC_2013.pdf: 550641 bytes, checksum: bc89614583d69ebc4f261c9139d11e83 (MD5)Made available in DSpace on 2019-12-03T15:59:22Z (GMT). No. of bitstreams: 1 JosePascoaSimonetti_etal_IOC_2013.pdf: 550641 bytes, checksum: bc89614583d69ebc4f261c9139d11e83 (MD5) Previous issue date: 2013Instituto Estadual de Diabetes e Endocrinologia Luiz Capriglione. Rio de Janeiro, RJ, Brasil.Instituto Estadual de Diabetes e Endocrinologia Luiz Capriglione. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Programa de PósGraduação em Fisiopatologia Clínica e Experimental. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Instituto Estadual de Diabetes e Endocrinologia Luiz Capriglione. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Programa de PósGraduação em Fisiopatologia Clínica e Experimental. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Avaliar características clínicas, bioquímicas, hormonais e genéticas de familiares de duas pacientes portadoras de lipodistrofia parcial familiar (FPLD) tipo 2.To evaluate clinical, biochemical, hormonal and genetic characteristics of relatives of two patients with familial partial lipodystrophy (FPLD) type 2

Description of a widespread outbreak of aseptic meningitis due to echovirus 30 in Rio de Janeiro state, Brazil

Echovirus 30 belongs to the genus Enterovirus and is widely associated with aseptic meningitis (AM) outbreaks. In Brazil epidemics due to this serotype were reported in several states but in Rio de Janeiro, before this study, it was only involved in sporadic episodes. We retrospectively collected data from AM notifications charts and enterovirus isolation database from Rio de Janeiro State Health Department (RJSHD) and Enterovirus Reference Laboratory in the year of 2005. An outbreak of AM was detected during March, April and May associated with a high cell culture isolation rate for echovirus 30 (17.4%). Male children with ages varying from 1 to 9 years were more affected. Of the 22 patients with confirmed echovirus 30 disease, clinical information was available in eight; fever, headache and vomiting were the most common manifestations. CSF analysis showed a typical pattern of viral infection with median of cellularity of 100 cells/mm³ and mononuclear cell predominance in 64.7% of the cases. The median of protein and glucose levels of 49 mg/dL and 56.5 mg/dL. The fatality rate was null. Despite its benign course and the lack of treatment options, aseptic meningitis surveillance is crucial for early identification of causative agents of outbreaks, which helps to avoid additional testing and inappropriate use of antimicrobials

Environmental Surveillance of Polioviruses in Rio de Janeiro, Brazil, in Support to the Activities of Global Polio Eradication Initiative

Submitted by sandra infurna ([email protected]) on 2016-05-31T14:04:36Z No. of bitstreams: 1 joseane_pereira_etal_IOC_2016.pdf: 401171 bytes, checksum: 9868d1a3bd496731ee2cb948c4459608 (MD5)Approved for entry into archive by sandra infurna ([email protected]) on 2016-06-02T13:32:43Z (GMT) No. of bitstreams: 1 joseane_pereira_etal_IOC_2016.pdf: 401171 bytes, checksum: 9868d1a3bd496731ee2cb948c4459608 (MD5)Made available in DSpace on 2016-06-02T13:32:43Z (GMT). No. of bitstreams: 1 joseane_pereira_etal_IOC_2016.pdf: 401171 bytes, checksum: 9868d1a3bd496731ee2cb948c4459608 (MD5) Previous issue date: 2016Made available in DSpace on 2016-06-03T12:34:15Z (GMT). No. of bitstreams: 2 joseane_pereira_etal_IOC_2016.pdf: 401171 bytes, checksum: 9868d1a3bd496731ee2cb948c4459608 (MD5) license.txt: 2991 bytes, checksum: 5a560609d32a3863062d77ff32785d58 (MD5) Previous issue date: 2016Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ, Brasil.Wild polioviruses still remain endemic in three countries (Afghanistan, Pakistan, and Nigeria) and re-emergency of wild polio has been reported in previously polio-free countries. Environmental surveillance has been used as a supplementary tool in monitoring the circulation of wild poliovirus (PVs) and/or vaccine-derived PVs even in the absence of acute flaccid paralysis cases. This study aimed to monitor the presence of polioviruses in wastewater samples collected at one wastewater treatment plant located in the municipality of Rio de Janeiro, Brazil. From December 2011 to June 2012 and from September to December 2012, 31 samples were collected and processed. RD and L20B cell cultures were able to isolate PVs and non-polio enteroviruses in 27/31 samples. Polioviruses were isolated in eight samples (type 1 Sabin = 1, type 2 Sabin = 5, and type 3 Sabin = 2). Vaccine-derived polioviruses were not detected nor evidence of recombination with other PVs or non-polio enterovirus serotypes were observed among the isolates. The Sabin-related serotypes 2 and 3 presented nucleotide substitutions in positions associated with the neurovirulent phenotype at the 5'-UTR. Changes in important Amino acid residues at VP1 were also observed in the serotypes 2 and 3. Environmental surveillance has been used successfully in monitoring the circulation of PVs and non-polio enteroviruses and it is of crucial importance in the final stages of the WHO global polio eradication initiative. Our results show the continuous circulation of Sabin-like PVs and non-polio enteroviruses in the analyzed area during the study period