Encouraging urban planning and development practice to contribute to sustainable development. A methodology and a use case

One of the most complex challenges the tourism industry faces is keeping up to date with information technology developments caused by the globalisation of information and advances in technology. The development of robust decision support systems for tourism land use planning is a way to address this challenge.This paper demonstrates how a spatial decision support system (SDSS), called the Land Use Decision sUpport System (LUDUS), can contribute in allocating complex forms of tourism. The system combines an artificial intelligence technique, called ontologies, with Geographic Information Systems and object-oriented programming to support decision-making in spatial planning. The system consists of two subsystems: the Insert Data Subsystem and the Graphic Imaging and Decision Support Subsystem. The core of the system is an ontology that is aligned to a standard of the Open Geospatial Consortium, called Geosparql.The case study of this paper is the Mastichochoria area of Chios Island, Greece. Therefore, the structure of the ontology was modelled according to the provisions of Greek legislation. The results produced confirmed the correct coding and application of the system’s criteria. The validity, accuracy and reliability of the results were also confirmed.The adopted approach facilitates the identification of alternative options for allocating, among other land use types, complex forms of tourism development in suburban areas, by examining the provisions of the legal framework as well as their geology and terrain

The Use of Stromal Vascular Fraction in Long Bone Defect Healing in Sheep

The objectives of the present study were to evaluate (a) the feasibility of using stromal vascular fraction (SVF) and nanocrystalline hydroxyapatite (nHA) paste in combination for the treatment of segmental bone defect, (b) the quality of the callus produced, (c) the potential improvement of the autograft technique, and (d) the direct comparison of the biomaterial to the use of autogenous cancellous bone. Unilateral, segmental mid-diaphyseal bone defect was created on the right metatarsus of skeletally mature sheep animals (n = 24) under anesthesia (D0). Residual segments were stabilized by stainless-steel plates and appropriate screws. Defects were managed as follows: group A: use of nHA paste to filling, group B: use of autogenous bone graft mixed with nHA bone paste, placed in defect, group C: use of SVF mixed with nHA bone paste injected into defect, group D: use of bone graft and SVF with nHA paste before apposition in bone defect. SVF had been previously isolated from adipose tissue of the animals intra-operatively after digestion with collagenase solution and neutralization. Animals were evaluated clinically and by X-raying and ultrasonographic examination of the defect, at regular intervals, until D90. Ultrasonographic assessment performed along the length of the defect included calculation of the length of the bone defect and assessment of vascularization. SVF was successfully isolated from group C and D animals, with the average yield being 1.77 × 106 cells. The comparison of clinical scores (based on the ‘Kaler scale’) on each post-operative day indicated significant differences between the four groups on D1 to D30 (p p = 0.07) and 0 (p = 0.033). Differences in radiographic assessment scores were significant for scores obtained on D60 (p = 0.049) and D90 (p = 0.006). There was a significant difference between the four groups in the length of the bone defect, as assessed ultrasonographically, for the entire length of the study; median values were 8, 8.5, 6, and 8 mm for groups A, B, C, and D, respectively (p = 0.008). There was a significance in the differences between median scores obtained during the histopathological examination: 2, 11, 13.5, and 12 for group A, B, C, and D (p = 0.022). There was an inverse correlation between the overall scores of histopathological evaluations and the length of the bone defect (observed on D90) (p p < 0.0001). This is the first study in which the efficacy of fresh autologous Stromal Vascular Fraction (SVF) from adipose tissue in enhancing bone healing in a long, weight-bearing, diaphyseal bone was evaluated. It is concluded that the lumbosacral region was an attractive site for harvesting adipose tissue, the use of SVF contributed to faster rehabilitation post-operatively, and SVF significantly enhanced bone formation; in general, the results indicated an osteogenic potential of SVF comparable to the gold standard autologous bone graft