Elevated Airway Purines in COPD

Adenosine and related purines have established roles in inflammation, and elevated airway concentrations are predicted in patients with COPD. However, accurate airway surface purine measurements can be confounded by stimulation of purine release during collection of typical respiratory samples

The Relationship of Mucus Concentration (Hydration) to Mucus Osmotic Pressure and Transport in Chronic Bronchitis

Rationale: Chronic bronchitis (CB) is characterized by persistent cough and sputum production. Studies were performed to test whether mucus hyperconcentration and increased partial osmotic pressure, in part caused by abnormal purine nucleotide regulation of ion transport, contribute to the pathogenesis of CB

Avian Influenza Virus Glycoproteins Restrict Virus Replication and Spread through Human Airway Epithelium at Temperatures of the Proximal Airways

Transmission of avian influenza viruses from bird to human is a rare event even though avian influenza viruses infect the ciliated epithelium of human airways in vitro and ex vivo. Using an in vitro model of human ciliated airway epithelium (HAE), we demonstrate that while human and avian influenza viruses efficiently infect at temperatures of the human distal airways (37°C), avian, but not human, influenza viruses are restricted for infection at the cooler temperatures of the human proximal airways (32°C). These data support the hypothesis that avian influenza viruses, ordinarily adapted to the temperature of the avian enteric tract (40°C), rarely infect humans, in part due to differences in host airway regional temperatures. Previously, a critical residue at position 627 in the avian influenza virus polymerase subunit, PB2, was identified as conferring temperature-dependency in mammalian cells. Here, we use reverse genetics to show that avianization of residue 627 attenuates a human virus, but does not account for the different infection between 32°C and 37°C. To determine the mechanism of temperature restriction of avian influenza viruses in HAE at 32°C, we generated recombinant human influenza viruses in either the A/Victoria/3/75 (H3N2) or A/PR/8/34 (H1N1) genetic background that contained avian or avian-like glycoproteins. Two of these viruses, A/Victoria/3/75 with L226Q and S228G mutations in hemagglutinin (HA) and neuraminidase (NA) from A/Chick/Italy/1347/99 and A/PR/8/34 containing the H7 and N1 from A/Chick/Italy/1347/99, exhibited temperature restriction approaching that of wholly avian influenza viruses. These data suggest that influenza viruses bearing avian or avian-like surface glycoproteins have a reduced capacity to establish productive infection at the temperature of the human proximal airways. This temperature restriction may limit zoonotic transmission of avian influenza viruses and suggests that adaptation of avian influenza viruses to efficient infection at 32°C may represent a critical evolutionary step enabling human-to-human transmission

Temperature-dependent growth of different serotypes of influenza viruses in HAE.

<p>Multi-step growth kinetics of (A) human influenza virus A/Eng/26/99 or (C) avian influenza virus A/Dk/Sing/97 (MOI∼0.1) at 32°C (<i>open circles</i>, <i>dashed line</i>) or 37°C (<i>closed circles</i>, <i>solid line</i>) in HAE +/−SE (n = 3 cultures). Multi-step growth kinetics in HAE inoculated with an MOI∼0.03 of (B) A/Udorn/307/72 (H3N2) or (D) A/VN/1203/04 (H5N1) at 33°C (<i>open circles</i>, <i>dashed line</i>) or 37°C (<i>closed circles</i>, <i>solid line</i>). Data represents mean titer across two different donors, each performed in duplicate +/−SE. Viral titers were determined by plaque assay in (A) and (B) and by TCID₅₀ assay for (C) and (D). No significant differences in growth between temperatures were found for either A/Eng/26/99 or A/Udorn/307/72. A/VN/1203/04 was significantly restricted for growth at 24, 48 and 72 hrs pi (*p<0.05). (E) Representative histological cross-sections of HAE infected for 72 hrs at 37°C with A/Udorn/307/72 or A/VN/1203/04 and compared to mock-inoculated HAE. H&E counterstain. Scale bar equals 20 µm.</p

Temperature restriction of avian influenza viruses at 32°C can be mimicked by inserting avian envelope glycoproteins into human influenza viruses.

<p>(A) Multi-step growth kinetics initiated in HAE over time with PR8+Vic HA/NA at 32°C (<i>closed triangles</i>) or 37°C (<i>open triangles</i>) and PR8+Chick HA/NA at 32°C (<i>closed circles</i>) or 37°C (<i>open circle</i>s) in HAE. Apical viral titers were determined at the times shown by standard plaque assay. Data shown represents mean titer across 4–8 cultures +/−SE. (B) Adenylate kinase activity in apical washes of virus-infected HAE expressed as fold-change over adenylate kinase activity in mock-inoculated HAE +/−SE (n = 4–8). Significance is noted (*p<0.05) where viral titers or AK levels obtained for PR8+Chick HA/NA at 32°C were statistically different from all other titers/AK measurements (Chick/37°C, Vic/32°C and Vic/37°C) at that particular time point. Significance is noted (^†p<0.05) where AK levels obtained for PR8+Chick HA/NA at 32°C and 37°C were statistically different. (C,D) Representative <i>en face</i> photomicrographs of viral nucleoprotein immunoreactivity (<i>green</i>) in HAE inoculated with (C) PR8+Vic HA/NA or (D) PR8+Chick HA/NA, at 24, 48 and 72 hrs pi at 32°C (lower rows) or 37°C (upper rows).</p

Infection of HAE by avian, but not human, influenza viruses is restricted at temperatures of the proximal airways.

<p>(A) Comparison of multi-cycle virus growth in HAE inoculated with either A/Victoria/3/75 at 32°C (<i>closed triangles</i>) or 37°C (<i>open triangles</i>) and A/Dk/Eng/62 at 32°C (<i>closed circles</i>) or 37°C (<i>open circles</i>) both at MOI∼0.01. Apical viral titers at times shown were determined by standard plaque assay on MDCK cells. Data shown represents the mean titer +/−standard error (SE; n = 3–10 cultures). (B) Adenylate kinase activity released into the apical compartment of HAE over time after inoculation with A/Victoria/3/75 or A/Dk/Eng/62 at 32°C and 37°C as a measure of viral-induced CPE. Data shown represents the mean fold change over adenylate kinase activity derived from mock-inoculated HAE +/−SE (n = 3–8). Significance is noted (*p<0.05) where viral titers or AK levels obtained for A/Dk/Eng/62 at 32°C were statistically different from all other titers/AK measurements (Dk/37°C, Vic/32°C and Vic/37°C) at that particular time point. Significance is noted (^†p<0.05) where AK levels obtained for A/Dk/Eng/62 at 32°C and 37°C were statistically different.</p

Cell tropism of human, avian and avianized viruses in HAE.

<p>Representative cross-sections of inoculated HAE, fixed 24 hrs pi, were probed for viral antigen (NP; green) and α−acetylated tubulin, a marker for ciliated cells (red). Notably, the staining pattern for wild-type A/Victoria/3/75 was identical to that of PR8+Vic HA/NA. Arrows mark ciliated cells infected with either wild-type A/Victoria/3/75 or PR8+Vic HA/NA; arrow-head denotes non-ciliated cells infected by these viruses. These data indicate that viruses with Victoria glycoproteins were able to infect both cell types previously shown to express α2,6 SA <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1000424#ppat.1000424-Thompson1" target="_blank">[13]</a>. Viral antigen was detected only in ciliated cells in cultures inoculated with Vic-226-228HA (in the Victoria background with either endogenous N2 or avian N1 or PR8+Chick HA/NA). Scale bar equals 20 µm.</p

Spread and histopathology of avian and human influenza viruses in HAE at temperatures of the proximal and distal airway.

<p>(A) Representative <i>en face</i> photomicrographs of HAE inoculated with either A/Victoria/3/75 or A/Dk/Eng/62 at 32°C or 37°C, fixed at 6, 24, 48 and 72 hrs pi and stained for viral nucleoprotein (<i>green</i>) to determine numbers of cells infected. Scale bar equals 100 µm. (B) Representative histological cross-sections of HAE at 24, 72 and 120 hrs after inoculation with A/Victoria/3/75 or A/Dk/Eng/62 at 32°C or 37°C. H&E counterstain. Scale bar equals 20 µm.</p

The Relationship of Mucus Concentration (Hydration) to Mucus Osmotic Pressure and Transport in Chronic Bronchitis

Rationale: Chronic bronchitis (CB) is characterized by persistent cough and sputum production. Studies were performed to test whether mucus hyperconcentration and increased partial osmotic pressure, in part caused by abnormal purine nucleotide regulation of ion transport, contribute to the pathogenesis of CB. Objectives: We tested the hypothesis that CB is characterized by mucus hyperconcentration, increased mucus partial osmotic pressures, and reduced mucus clearance. Methods: We measured in subjects with CB as compared with normal and asymptomatic smoking control subjects indices of mucus concentration (hydration; i.e., percentage solids) and sputum adenine nucleotide/nucleoside concentrations. In addition, sputum partial osmotic pressures and mucus transport rates were measured in subjects with CB. Measurements and Results: CB secretions were hyperconcentrated as indexed by an increase in percentage solids and total mucins, in part reflecting decreased extracellular nucleotide/nucleoside concentrations. CB mucus generated concentration-dependent increases in partial osmotic pressures into ranges predicted to reduce mucus transport. Mucociliary clearance (MCC) in subjects with CB was negatively correlated with mucus concentration (percentage solids). As a test of relationships between mucus concentration and disease, mucus concentrations and MCC were compared with FEV(1), and both were significantly correlated. Conclusions: Abnormal regulation of airway surface hydration may slow MCC in CB and contribute to disease pathogenesis