3 research outputs found
Human kallikrein-related peptidase 12 (KLK12) splice variants expression in breast cancer and their clinical impact
Kallikrein-related peptidases (KLKs) are a group of 15 serine proteases,
hormonally regulated, and localized on chromosome 19q13.4. Alternative
splicing is a process that plays significant role in the development,
physiology, and different diseases, like cancer. Kallikrein family
numbers more than 82 alternative transcripts. Understanding the role
that those gene transcripts play in various cancer types, could lead to
the discovery of diagnostic markers or drug targets. The present study
was designed to analyze the expression profile of the splice variants of
kallikrein-related peptidase 12 (KLK12) in breast cancer patients and to
evaluate their clinical significance. KLK12 splice variants (KLK12sv3
and KLK12sv1/KLK12sv2) were examined in 69 tissue samples of breast
cancer using quantitative real-time PCR as well as semi-quantitative
PCR. Relative quantitative expression of KLK12 was statistically
associated to clinicopathological parameters. From the splice variants
examined, statistical associations with clinicopathological parameters
were obtained only from KLK12sv3 variant. KLK12sv3 is more frequently
expressed in tumors of lower grade (p = 0.040), early patient TNM stage
(p = 0.024), and smaller tumor size (p = 0.023). Positive KLK12sv3
expression is associated with longer patient disease-free survival (DFS)
(p = 0.042) and higher progesterone receptor concentration (p = 0.008).
KLK12sv1/KLK12sv2 expression is statistically associated with KLK12sv3
expression (p = 0.001). KLK12sv3 can be regarded as a marker of good
prognosis in breast cancer
Synthesis and Characterization of Mesoporous Mg- and Sr-Doped Nanoparticles for Moxifloxacin Drug Delivery in Promising Tissue Engineering Applications
International audienceMesoporous silica-based nanoparticles (MSNs) are considered promising drug carriers because of their ordered pore structure, which permits high drug loading and release capacity. The dissolution of Si and Ca from MSNs can trigger osteogenic differentiation of stem cells towards extracellular matrix calcification, while Mg and Sr constitute key elements of bone biology and metabolism. The aim of this study was the synthesis and characterization of sol–gel-derived MSNs co-doped with Ca, Mg and Sr. Their physico-chemical properties were investigated by X-ray diffraction (XRD), scanning electron microscopy with energy dispersive X-ray analysis (SEM/EDX), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), X-ray fluorescence spectroscopy (XRF), Brunauer Emmett Teller and Brunauer Joyner Halenda (BET/BJH), dynamic light scattering (DLS) and ζ-potential measurements. Moxifloxacin loading and release profiles were assessed with high performance liquid chromatography (HPLC) cell viability on human periodontal ligament fibroblasts and their hemolytic activity in contact with human red blood cells (RBCs) at various concentrations were also investigated. Doped MSNs generally retained their textural characteristics, while different compositions affected particle size, hemolytic activity and moxifloxacin loading/release profiles. All co-doped MSNs revealed the formation of hydroxycarbonate apatite on their surface after immersion in simulated body fluid (SBF) and promoted mitochondrial activity and cell proliferation