287 research outputs found
A new species of mudfish, Neochanna (Teleostei: Galaxidae), from northern New Zealand
A new species of mudfish, Neochanna, is described from Northland. Neochanna heleios n.sp. is known from only three ephemeral wetland sites on the Kerikeri volcanic plateau and is abundant only at the type locality. The new species has a head resembling that of the brown mudfish, Neochanna apoda, and a caudal region resembling that of the black mudfish, Neochanna diversus. It can be distinguished from all Neochanna species in having a reduced number of principal caudal fin rays (13 or less). Morphometric and meristic comparisons with N. apoda and N. diversus are provided
Electrical Conductance of Molecular Wires
Molecular wires (MW) are the fundamental building blocks for molecular
electronic devices. They consist of a molecular unit connected to two continuum
reservoirs of electrons (usually metallic leads). We rely on Landauer theory as
the basis for studying the conductance properties of MW systems. This relates
the lead to lead current to the transmission probability for an electron to
scatter through the molecule. Two different methods have been developed for the
study of this scattering. One is based on a solution of the Lippmann-Schwinger
equation and the other solves for the {\bf t} matrix using Schroedinger's
equation. We use our methodology to study two problems of current interest. The
first MW system consists of 1,4 benzene-dithiolate (BDT) bonded to two gold
nanocontacts. Our calculations show that the conductance is sensitive to the
chemical bonding between the molecule and the leads. The second system we study
highlights the interesting phenomenon of antiresonances in MW. We derive an
analytic formula predicting at what energies antiresonances should occur in the
transmission spectra of MW. A numerical calculation for a MW consisting of
filter molecules attached to an active molecule shows the existence of an
antiresonance at the energy predicted by our formula.Comment: 14 pages, 5 figure
Inhibition of RNA polymerase II transcription in human cells by synthetic DNA-binding ligands
Sequence-specific DNA-binding small molecules that can permeate human cells potentially could regulate transcription of specific genes. Multiple cellular DNA-binding transcription factors are required by HIV type 1 for RNA synthesis. Two pyrrole-imidazole polyamides were designed to bind DNA sequences immediately adjacent to binding sites for the transcription factors Ets-l, lymphoid-enhancer binding factor 1, and TATA-box binding protein. These synthetic ligands specifically inhibit DNA-binding of each transcription factor and HIV type 1 transcription in cell-free assays. When used in combination, the polyamides inhibit virus replication by >99% in isolated human peripheral blood lymphocytes, with no detectable cell toxicity, The ability of small molecules to target predetermined DNA sequences located within RNA polymerase II promoters suggests a general approach for regulation of gene expression, as well as a mechanism for the inhibition of viral replication
Antiresonances in Molecular Wires
We present analytic and numerical studies based on Landauer theory of
conductance antiresonances of molecular wires. Our analytic treatment is a
solution of the Lippmann-Schwinger equation for the wire that includes the
effects of the non-orthogonality of the atomic orbitals on different atoms
exactly. The problem of non-orthogonality is treated by solving the transport
problem in a new Hilbert space which is spanned by an orthogonal basis. An
expression is derived for the energies at which antiresonances should occur for
a molecular wire connected to a pair of single-channel 1D leads. From this
expression we identify two distinct mechanisms that give rise to antiresonances
under different circumstances. The exact treatment of non-orthogonality in the
theory is found to be necessary to obtain reliable results. Our numerical
simulations extend this work to multichannel leads and to molecular wires
connected to 3D metallic nanocontacts. They demonstrate that our analytic
results also provide a good description of these more complicated systems
provided that certain well-defined conditions are met. These calculations
suggest that antiresonances should be experimentally observable in the
differential conductance of molecular wires of certain types.Comment: 22 pages, 5 figure
State Orthogonalization by Building a Hilbert Space: A New Approach to Electronic Quantum Transport in Molecular Wires
Quantum descriptions of many complex systems are formulated most naturally in
bases of states that are not mutually orthogonal. We introduce a general and
powerful yet simple approach that facilitates solving such models exactly by
embedding the non-orthogonal states in a new Hilbert space in which they are by
definition mutually orthogonal. This novel approach is applied to electronic
transport in molecular quantum wires and is used to predict conductance
antiresonances of a new type that arise solely out of the non-orthogonality of
the local orbitals on different sites of the wire.Comment: 4 pages 1 figur
Current-Driven Conformational Changes, Charging and Negative Differential Resistance in Molecular Wires
We introduce a theoretical approach based on scattering theory and total
energy methods that treats transport non-linearities, conformational changes
and charging effects in molecular wires in a unified way. We apply this
approach to molecular wires consisting of chain molecules with different
electronic and structural properties bonded to metal contacts. We show that
non-linear transport in all of these systems can be understood in terms of a
single physical mechanism and predict that negative differential resistance at
high bias should be a generic property of such molecular wires.Comment: 9 pages, 4 figure
Electron Standing Wave Formation in Atomic Wires
Using the Landauer formulation of transport theory and tight binding models
of the electronic structure, we study electron transport through atomic wires
that form 1D constrictions between pairs of metallic nano-contacts. Our results
are interpreted in terms of electron standing waves formed in the atomic wires
due to interference of electron waves reflected at the ends of the atomic
constrictions. We explore the influence of the chemistry of the atomic
wire-metal contact interfaces on these standing waves and the associated
transport resonances by considering two types of atomic wires: gold wires
attached to gold contacts and carbon wires attached to gold contacts. We find
that the conductance of the gold wires is roughly for the
wire lengths studied, in agreement with experiments. By contrast, for the
carbon wires the conductance is found to oscillate strongly as the number of
atoms in the wire varies, the odd numbered chains being more conductive than
the even numbered ones, in agreement with previous theoretical work that was
based on a different model of the carbon wire and metal contacts.Comment: 14 pages, includes 6 figure
Orthostatic responses to dietary sodium restriction during heat acclimation
Several studies have shown that individuals consuming low-salt diets and working in the heat have an increased risk or incidence of heat injury, suggestive of inadequate cardiovascular adjustment. Furthermore, others have shown that prolonged work in hot climates can precipitate orthostatic hypotension and syncope. This study was designed to evaluate the effects of moderate-salt (MS) and low-salt (LS) diets on the circulatory responses and incidence of presyncopal symptoms to an orthostatic test (OT) during successive days of heat acclimation (HA). Seventeen unacclimatized male soldiers (mean +/- SE: age 20+/-1 yrs) participated in this two-phase study. The first phase consisted of a seven day dietary stabilization period during which all subjects consumed similar diets of about 4000 kcal/day containing 8g NaCl and lived in a dormitory setting (21 C, 30% RH). The second phase commenced on day eight and consisted of dietary NaCl restriction and 10 days HA (days 8-17). Volunteers were randomly assigned to either the MS diet (n=9) providing 8g NaCl/day or the LS diet (n=8) furnishing just 4g NaCl/day. The acquisition of HA was manifested in both groups by reductions in exercising rectal temperature and heart rate (HR); these characteristics were similar in the MS and LS diets. The OT was performed at 21 C on day seven of the stabilization phase and on days 9, 11, 13, 15, and 17 of the HA phase, before and after 8.5 hr of intermittent treadmill walking in a hot environment. Blood pressure (BP) and HR responses at 1,2, and 4 min and any presyncopal symptoms were recorded after assuming an upright position from recumbency. All subjects completed the OT before and after prolonged exercise in the heat without incidence of either hypotension or presyncopal symptoms irrespective of dietary-salt intake and day of HA. The results indicate that the prolonged work in the heat can be performed without orthostatic hypotension or syncope while consuming 4g NaCl/day with adequate fluid replacement. Furthermore, the circulatory responses to OT showed no improvement with successive days of HA irrespective of dietary-salt intake
Inhibition of Ets-1 DNA Binding and Ternary Complex Formation between Ets-1, NF-kappa B, and DNA by a Designed DNA-binding Ligand
Sequence-specific pyrrole-imidazole polyamides can be designed to interfere with transcription factor binding and to regulate gene expression, both in vitro and in living cells. Polyamides bound adjacent to the recognition sites for TBP, Ets-1, and LEF-1 in the human immunodeficiency virus, type 1 (HIV-1), long terminal repeat inhibited transcription in cell-free assays and viral replication in human peripheral blood lymphocytes. The DNA binding activity of the transcription factor Ets-1 is specifically inhibited by a polyamide bound in the minor groove. Ets-1 is a member of the winged-helix-turn-helix family of transcription factors and binds DNA through a recognition helix bound in the major groove with additional phosphate contacts on either side of this major groove interaction. The inhibitory polyamide possibly interferes with phosphate contacts made by Ets-1, by occupying the adjacent minor groove. Full-length Ets-1 binds the HIV-1 enhancer through cooperative interactions with the p50 subunit of NF-kappa B, and the Ets-inhibitory polyamide also blocks formation of ternary Ets-1·NF-kappa B·DNA complexes on the HIV-1 enhancer. A polyamide bound adjacent to the recognition site for NF-kappa B also inhibits NF-kappa B binding and ternary complex formation. These results broaden the application range of minor groove-binding polyamides and demonstrate that these DNA ligands are powerful inhibitors of DNA-binding proteins that predominantly use major groove contacts and of cooperative protein-DNA ternary complexes
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