Quality of Life and obesity among Female Employee at Assiut University

Egypt is one of the countries where the problem of obesity has been nearing an epidemic level. Currently, nearly 70% of adult women and 48% of men in Egypt are overweight or obese. This matter should be taken seriously because it can burden the health care system and lower the quality of life. Aim of the study: To identify the relationship between quality of Life and obesity among female employee at Assiut University. Subject and Method: descriptive correlation design was utilized to carry out this study. Sample: The studied sample was consisted of 1000 female. Data were collected by using three tools; the first tool is self administered questionnaire includes socio-demographic characteristics of the studied sample. The second tool was an Anthropometric Measurements (weight, height and BMI) and the third tool was the World Health Organization Quality of life (WHOQOL-BREF). Results: the mean age was 36.97 ± 10.80. There was statistical significant difference between Socio-demographic characteristic such as age, marital status, educational level and obesity, also BMI effect on the three QOL domains (physical, psychological and social) while there was no significance differences with environmental aspects of QOL. Conclusion: Obesity has a negative effect on QOL among obese female employees. Recommendation: Awareness about nutritional needs and balanced diet should be increased for every age group through mass media. As well as developing a health education programs about balanced diet and prevention of obesity. Also regular exercise should be included within the daily routine activities of the female employees. Keywords: Quality Of Life (QOL), Obesity, Body Mass Index and Nursing Intervention

Knowledge and practice of Caregivers about Intestinal Parasitic Infestations at EL- Minia City

Intestinal infestations are amongst the common infections worldwide, it is estimated that about 3.5 billion people are affected, and that 450 million are ill as a result of these infestations, lead to physical and mental health problems such as iron deficiency anaemia, growth retardation, and lack of concentration the majority are being children that .Aim of the study: The study aimed to assess the knowledge and practices of caregivers about intestinal parasitic infestations at EL-Minia City. Subjects and methods: Descriptive research design was used in this study to identify the knowledge and practices of caregivers about intestinal parasitic infestations; the study was conducted in four primary governmental   schools. These were Saad Zaglol, Tark ebn Ziad, Elfath and EL-shaheed schools at    EL-Minia city.  Tool of the study included a structured interview sheet  it include two parts a) the demographic characteristics it includes (child age, child sex, residence, education and occupation of caregivers …….etc)The Caregivers knowledge regarding the intestinal infestation and consists of the following: (definition, types of intestinal worm, cycle of Pin worm and Ascariasis, mode of transmission, prevention, treatment B: The second tool (observational check list): was developed for assessing house environmental sanitation and assessing the caregiver's practices. Results: There was a highly statistically significant difference between the caregivers’ and their knowledge about intestinal infestation. Also, there with highly statistically significant difference between caregivers and their practices this study found that significant improvement of knowledge, practices of caregivers regarding intestinal parasitic infestations. Recommendations: Encourage the role of the community health nurse in health education for prevention and controlling of intestinal infestation. Keywords: intestinal infestation, caregiver knowledge. Practices 

Use of inter simple sequence repeats and protein markers in assessing genetic diversity and relationships among four rabbit genotypes

[EN] The importance of DNA and protein applications as powerful tools in breeding programmes is revealed. The inter simple sequence repeats (ISSR) technique was used to characterise and determine phylogenetic relationships among 4 genotypes of rabbit, namely Alexandria (Alex), V line (V), New Zealand White (NZW) and California (Cal). Six out of 7 ISSR primers exhibited sufficient variability and were used to characterise the genetic diversity and relationships among studied genotypes. A total of 141 DNA  ands were detected. DNA fragments were generated with 87 (61.7%) being polymorphic, indicating considerable genetic variation among the examined genotypes. While protein electrophoresis provides a precise method for assaying variation in serum proteins which play an important part in productive performance. The results demonstrated 2 specific protein markers in Alexandria rabbits; these specific protein markers may be responsible for the superiority of Alexandria line in weight. Phylogenetic analysis based on Nei and Li unbiased genetic distance illustrated that (Alex & V) and (Cal & V) were genetically closely related. Our results showed that ISSR and protein electrophoresis are useful methods to detect different genetic expressions and understand the variability in some productive traits in rabbits.El-Sabrout, K.; Aggag, S. (2015). Use of inter simple sequence repeats and protein markers in assessing genetic diversity and relationships among four rabbit genotypes. World Rabbit Science. 23(4):283-288. doi:10.4995/wrs.2015.3912.SWORD28328823

Medical students' satisfaction regarding blended learning during COVID-19 pandemic

Background: The realization of the profession’s role in both of global and personal concept affected the self-identification among 2020 medical students. COVID-19 pandemic can hinder, accelerate, or change medical students’ professional identity formation. Objective: To assess the level of satisfaction about blended learning among undergraduate medical students at Zagazig University and to determine challenges, which face the medical students during blended learning education in Faculty of Medicine. Patients and Methods: This cross-sectional study was performed during the period from September 2020 to July 2021 at Zagazig University, Egypt. This research included 277 undergraduate medical students. Results: The current study reported that interaction satisfaction score ranged from 18 to 53 with median 37 and 53.1% of students reported high satisfaction with interaction domain. There was statistically significant correlation between total satisfaction score and both age and technology barrier. There was statistically non-significant correlation between total satisfaction score and communication barrier. Conclusion: The results show that the majority of our sample students were highly satisfied with blended learning and reported low barriers to e-learning. Students still prefer face-to-face courses despite their satisfaction with their grades and performance in blended learning courses

Screening for Celiac Disease in Children with Dental Enamel Defects

Background. Dental enamel defects (DEDs) are seen in celiac disease (CD). Aim was to detect frequency of CD among such patients. Methods. This study included 140 children with DED. They were tested for CD. Gluten-free diet (GFD) was instituted for CD patients. A cohort of 720, age and sex-matched, normal children represented a control group. Both groups were evaluated clinically. Serum calcium, phosphorus, alkaline phosphatase, serum IgA, and tissue transglutaminase (tTG) IgG and IgA types were measured. Results. CD was more diagnosed in patients with DEDs (17.86%) compared to controls (0.97%) (P < 0.0001). Majority of nonceliac patients showed grade 1 DED compared to grades 1, 2, and 3 DED in CD. Five children had DED of deciduous teeth and remaining in permanent ones. After 1 year on GFD, DED improved better in CD compared to nonceliac patients. Gastrointestinal symptoms did not vary between celiac and nonceliac DED patients. Lower serum calcium significantly predicted CD in this cohort. Conclusion. CD is more prevalent among children with DED than in the general population. These DEDs might be the only manifestation of CD; therefore, screening for CD is highly recommended among those patients especially in presence of underweight and hypocalcemia

Synthesis and antitumor testing of certain new fused triazolopyrimidine and triazoloquinazoline derivatives

AbstractNew series of 1,2,4-triazolopyrimidine and 1,2,4-triazoloquinazoline derivatives were designed, synthesized, and evaluated for their antitumor activity. Compounds 6, 11, 26, 29, 41, 44, 48, 49 and 58 were tested as antitumor agents by the use of DNA-binding assay on TLC-plates, colorimetric assay for the degree of DNA-binding (Methyl green-DNA displacement assay), evaluation of antineoplastic activity against Ehrlich Ascites Carcinoma in mice, and finally modulation of apoptosis. 5-Flurouracil, vitamin C and ethidium bromide were used as positive controls in these techniques. Compound 26 proved to be the most active member of these series as antitumor agent with IC50 value of 47±1. Several characteristic features were observed to be essential for activity such as the morpholine group and the phenylazo group, in addition the electron-withdrawing groups favor the activity than the electron-donating ones

Evaluation of the Antimicrobial Activity of Four Plant Essential Oils against Some Food and Phytopathogens Isolated from Processed Meat Products in Egypt

Synthetic preservatives are widely utilized by the food industry to inhibit the microbial contamination and increase food safety and shelf life. The excessive utilization of synthetic preservatives can have a negative impact on human health and the environment. There is a great interest to find out natural substances as possible food-preservatives. The consumers' preference for food products with natural ingredients prompted food manufacturers to utilize natural-based preservatives in their production. It is worth noting that plant essential oils (EOs) among the natural-based substances have been efficiently used as antimicrobial agents against phyto- and food pathogens. The current study was conducted to evaluate the microbial contamination of three industrial meat products from five governorates in Egypt, identify the predominant bacterial and fungal isolates and determine the antimicrobial efficacy of some EOs (thyme, fennel, anise and marjoram) against the most predominant microbial isolates. A sensory test was also performed to estimate the customer preferences for specific organoleptic aspects of meat products after EOs treatment. Results showed that there is a promising antimicrobial activity of all studied EOs against some microbial isolates in a dose-dependent manner. In particular, thyme EO showed the highest significant antibacterial activity against P. fluorescence and E. coli. Whereas the marjoram EO showed the highest activity against P. aeruginosa. In addition, the sensory test revealed that the treatment with anise and marjoram EOs showed the highest acceptability by the testers and did not show significant differences on the organoleptic properties with respect to control. As overall, the obtained results of the current research are promising and proved feasibility of employing plant EOs as possible preservatives for processed meat products

Humoral and cellular immune responses to modified hepatitis B plasmid DNA vaccine in mice

Purpose: To evaluate the immunogenicity and types of immune response of a quality-controlled modified recombinant hepatitis B surface antigen (HBsAg) plasmid encoding HBsAg in mice.Methods: The characterized plasmid DNA was used in the immunization of Balb/c mice. Three groups of mice were intramuscularly injected with three different concentrations (50, 25 and 10 μg/100 μL) of the modified plasmid. Humoral immune response was monitored by enzyme-linked immunosorbent assay (ELISA), while cellular immune response was investigated by analysis of spleen cytokine profile (TNFα, IFN γ and IL2) as well as CD69 expression level in CD4 and CD8 positive cells.Results: In general, the activated CD4 cells showing intracellular cytokines were higher than CD8 positive population of cells (p &lt; 0.05). These findings indicate that the vaccine induced both a humoral and cellular immunity. Cytokine profile also showed high levels of TNFα, IFN γ and IL2 and CD69 expression in the group of animals immunized at a dose of 10 μg when compared to control group (p &lt; 0.05).Conclusion: A 10 μg dose intramuscular injection of the modified DNA-based vaccine encoding HBsAg in mice induces both high humoral and cellular immune responses.Keywords: Hepatitis B virus, Plasmid DNA, Vaccine, Spleen cytokines, Humoral and cellular immune response