The influence of cyclophosphamide on the reproduction of <i>D</i>. <i>magna</i>.

<p>Average (± SD) (a) age, (b) size at first reproduction and (c) first neonate number of <i>Daphnia</i> (Clones D, N and S) cultured with (grey bars) or without (white bars) cyclophosphamide. Stars indicate significant differences between the control and experimental groups.</p

Proteins with changed expressions in the N clone individuals which had been treated with CP, compared to control individuals, with q-value < 0.1 (ratio = control/CP treated animals).

<p>Proteins with changed expressions in the N clone individuals which had been treated with CP, compared to control individuals, with q-value < 0.1 (ratio = control/CP treated animals).</p

The influence of cyclophosphamide on the growth of <i>D</i>. <i>magna</i>.

<p>(a) Average ± SD growth rate of <i>Daphnia</i> clones D, N and e S on Day 5, cultured with (grey bars) or without (white bars) cyclophosphamide.</p

Common proteins with changed levels in <i>Daphnia</i> animals treated with cyclophosphamide.

<p>A Venn diagram showing common proteins, either (a) up- or (b) down regulated, in <i>Daphnia</i>, which changed significantly (q value < 0.1) in the clone D, N and S individuals which had been treated with cyclophosphamide.</p

The influence of cyclophosphamide on the growth of <i>D</i>. <i>magna</i>.

<p>(a) Average ± SD growth rate of <i>Daphnia</i> clones D, N and e S on Day 5, cultured with (grey bars) or without (white bars) cyclophosphamide.</p

The experimental design (description in the text).

<p>The experimental design (description in the text).</p

Proteins with changed expressions in the D clone individuals which had been treated with CP, compared to control individuals, with q-value < 0.1 (ratio = control (C)/CP treated animals).

<p>Proteins with changed expressions in the D clone individuals which had been treated with CP, compared to control individuals, with q-value < 0.1 (ratio = control (C)/CP treated animals).</p

Proteins with changed expressions in the S clone individuals which had been treated with CP, compared to control individuals, with q-value < 0.1 (ratio = control/CP treated animals).

<p>Proteins with changed expressions in the S clone individuals which had been treated with CP, compared to control individuals, with q-value < 0.1 (ratio = control/CP treated animals).</p

Subcellular localization of GFP-tagged AlkB homologs.

<p><i>A. thaliana</i> protoplasts from cell suspension culture were transfected with constructs expressing the indicated proteins in N- and C- terminal fusion with GFP and visualized by confocal laser-scanning microscopy. AlkB homologs represent 6 types of subcellular localization. The image for AtALKBH1D homolog localization is merged with the red autofluorescence of chlorophyll (orange color comes from overlay of GFP and chlorophyll fluorescence). For comparison GFP fluorescence alone (pSAT6-eGFP) was also analyzed. N - nucleus, Nu - nucleous, Ch - chloroplasts, V - vacuole. For more details see Supplementary Data.</p

Relocalization of <i>A. thaliana</i> nucleo-cytoplasmic AlkB homologs after LMB inhibition of nuclear export.

<p>Protoplasts transfected with GFP-AlkB homologs were incubated with LMB for 4 h and analyzed for GFP fluorescence using confocal laser-scanning microscopy. As a positive control plasmid expressing GFP-NLS-CHS-NES was used. All of the protoplasts transfected with AtALKBH6 and AtALKBH9C(l), and 30% of those transfected with AtALKBH8B changed their localization to exclusively nuclear after LMB treatment. In the case of AtALKBH9A and AtALKBH10B the signal in the nucleus was more intense after incubation with LMB, and LMB did not inhibit AtALKBH1A, AtALKBH8A, AtALKBH9C, AtALKBH6s and AtTRM9 export. The scans demonstrate the main localization of presented homologs.</p