450 research outputs found
Assessment of the Food-Swallowing Process Using Bolus Visualisation and Manometry Simultaneously in a Device that Models Human Swallowing
The characteristics of the flows of boluses with different consistencies, i.e. different rheological properties, through the pharynx have not been fully elucidated. The results obtained using a novel in vitro device, the Gothenburg Throat, which allows simultaneous bolus flow visualisation and manometry assessments in the pharynx geometry, are presented, to explain the dependence of bolus flow on bolus consistency. Four different bolus consistencies of a commercial food thickener, 0.5, 1, 1.5 and 2\ua0Pa\ua0s (at a shear rate of 50\ua0s â1 )âcorresponding to a range from low honey-thick to pudding-thick consistencies on the National Dysphagia Diet (NDD) scaleâwere examined in the in vitro pharynx. The bolus velocities recorded in the simulator pharynx were in the range of 0.046â0.48\ua0m/s, which is within the range reported in clinical studies. The corresponding wall shear rates associated with these velocities ranged from 13\ua0s â1 (pudding consistency) to 209\ua0s â1 (honey-thick consistency). The results of the in vitro manometry tests using different consistencies and bolus volumes were rather similar to those obtained in clinical studies. The in vitro device used in this study appears to be a valuable tool for pre-clinical analyses of thickened fluids. Furthermore, the results show that it is desirable to consider a broad range of shear rates when assessing the suitability of a certain consistency for swallowing
Simultaneous X-ray Video-Fluoroscopy and Pulsed Ultrasound Velocimetry Analyses of the Pharyngeal Phase of Swallowing of Boluses with Different Rheological Properties
The Ultrasound Velocity Profiling (UVP) technique allows real-time, non-invasive flow mapping of a fluid along a 1D-measuring line. This study explores the possibility of using the UVP technique and X-ray video-fluoroscopy (XVF) to elucidate the deglutition process with the focus on bolus rheology. By positioning the UVP probe so that the pulsed ultrasonic beam passes behind the air-filled trachea, the bolus flow in the pharynx can be measured. Healthy subjects in a clinical study swallowed fluids with different rheological properties: Newtonian (constant shear viscosity and non-elastic); Boger (constant shear viscosity and elastic); and shear thinning (shear rate-dependent shear viscosity and elastic). The results from both the UVP and XVF reveal higher velocities for the shear thinning fluid, followed by the Boger and the Newtonian fluids, demonstrating that the UVP method has equivalent sensitivities for detecting the velocities of fluids with different rheological properties. The velocity of the contraction wave that clears the pharynx was measured in the UVP and found to be independent of bolus rheology. The results show that UVP not only assesses accurately the fluid velocity in a bolus flow, but it can also monitor the structural changes that take place in response to a bolus flow, with the added advantage of being a completely non-invasive technique that does not require the introduction of contrast media
Posterior cricoid region fluoroscopic findings: the posterior cricoid plication.
The region posterior to the cricoid cartilage is challenging to assess fluoroscopically. The purpose of this investigation is to critically evaluate the posterior cricoid (PC) region on fluoroscopy and describe patterns of common findings. This was a case control study. All fluoroscopic swallowing studies performed between June 16, 2009, and February 9, 2010, were reviewed for features seen in the PC region. These findings were categorized into distinct patterns and compared to fluoroscopic studies performed in a cohort of normal volunteers. Two hundred patient studies and 149 healthy volunteer studies were reviewed. The mean age of the referred patient cohort and the volunteer cohort was 57 years (±19) and 61 years (±16), respectively (p > 0.05). The patient cohort was 53% male and the control cohort was 56% female (p > 0.05). Four groups were identified. Pharyngoesophageal webs were seen in 7% (10/149) of controls and 14% (28/200) of patients (p = 0.03). A PC arch impression was seen in 16% of patients (32/200) and controls (24/149) (p = 1). A PC plication was demonstrated in 23% (34/149) of controls and 30% (60/200) of patients (p = 0.13). No distinctive PC region findings were seen in 54% (81/149) of controls and 42% (84/200) of referred patients (p = 0.02). Four patients (2%) had both a web and a PC plication. Four categories of PC region findings were identified (unremarkable PC region, web, PC arch impression, and PC plication). Both patients referred for swallowing studies and healthy volunteers demonstrated esophageal webs, PC arch impressions, and PC plications. Only webs were more common in patients than in control subjects (p = 0.03). The PC impression and PC plication are likely to represent normal variants that may be identified on fluoroscopic swallow studies
Engineering tyrosine-based electron flow pathways in proteins: The case of aplysia myoglobin
Tyrosine residues can act as redox cofactors that provide an electron transfer ("hole-hopping") route that enhances the rate of ferryl heme iron reduction by externally added reductants, for example, ascorbate. Aplysia fasciata myoglobin, having no naturally occurring tyrosines but 15 phenylalanines that can be selectively mutated to tyrosine residues, provides an ideal protein with which to study such through-protein electron transfer pathways and ways to manipulate them. Two surface exposed phenylalanines that are close to the heme have been mutated to tyrosines (F42Y, F98Y). In both of these, the rate of ferryl heme reduction increased by up to 3 orders of magnitude. This result cannot be explained in terms of distance or redox potential change between donor and acceptor but indicates that tyrosines, by virtue of their ability to form radicals, act as redox cofactors in a new pathway. The mechanism is discussed in terms of the Marcus theory and the specific protonation/deprotonation states of the oxoferryl iron and tyrosine. Tyrosine radicals have been observed and quantified by EPR spectroscopy in both mutants, consistent with the proposed mechanism. The location of each radical is unambiguous and allows us to validate theoretical methods that assign radical location on the basis of EPR hyperfine structure. Mutation to tyrosine decreases the lipid peroxidase activity of this myoglobin in the presence of low concentrations of reductant, and the possibility of decreasing the intrinsic toxicity of hemoglobin by introduction of these pathways is discussed. © 2012 American Chemical Society
Mutational Analysis of the Analgesic Peptide DrTx(1-42) Revealing a Functional Role of the Amino-Terminal Turn
Background: DrTx(1-42) (a carboxyl-terminally truncated version of drosotoxin) is a potent and selective blocker of tetrodotoxin-resistant (TTX-R) Na + channels in rat dorsal root ganglion neurons with analgesic activity. This purpose is to identify key amino acids which are responsible for both blocking and analgesic effects of DrTx(1-42). Methods: On the basis of previous study, we designed five mutants of DrTx(1-42) (delN, D8A, D8K, G9A, and G9R) in the amino-terminal turn (N-turn) region, a proposed functional region located in the amino-terminus of the molecule. All these mutants were expressed in E.coli and purified by RP-HPLC. Electrophysiological properties of these analogues were examined by whole-cell patch-clamp recordings and their antinociceptive effects were investigated by the formalin test and acetic acid induced writhing test. Results: All the mutants except for G9A possess a similar secondary structure to that of DrTx(1-42), as identified by circular dichroism analysis. Three mutants (delN, D8A and G9A) were found almost inactive to TTX-R Na + channels, whereas D8K retains similar activity and G9R showed decreased potency when compared with the wild-type molecule. Consistent with the electrophysiological observations, D8K and G9R exhibited antinociceptive effects in the second phase (inflammatory pain) of the formalin test and the acetic acid induced writhing test, while delN, D8A and G9A lack such effects. Conclusions: Our results show that the N-turn is closely related to function of DrTx(1-42). The mutant (D8A) as a contro
Videoradiographic Analysis of How Carbonated Thin Liquids and Thickened Liquids Affect the Physiology of Swallowing in Subjects with Aspiration on Thin Liquids
Boron Abundances in Main Sequence B-type Stars: A Test of Rotational Depletion during Main Sequence Evolution
Boron abundances have been derived for seven main sequence B-type stars from
HST STIS spectra around the B III 2066 A line. In two stars, boron appears to
be undepleted with respect to the presumed initial abundance. In one star,
boron is detectable but it is clearly depleted. In the other four stars, boron
is undetectable implying depletions of 1 to 2 dex. Three of these four stars
are nitrogen enriched, but the fourth shows no enrichment of nitrogen. Only
rotationally induced mixing predicts that boron depletions are unaccompanied by
nitrogen enrichments. The inferred rate of boron depletion from our
observations is in good agreement with these predictions. Other boron-depleted
nitrogen-normal stars are identified from the literature. Also, several
boron-depleted nitrogen-rich stars are identified, and while all fall on the
boron-nitrogen trend predicted by rotationally-induced mixing, a majority have
nitrogen enrichments that are not uniquely explained by rotation.
The spectra have also been used to determine iron-group (Cr, Mn, Fe, and Ni)
abundances. The seven B-type stars have near solar iron-group abundances, as
expected for young stars in the solar neighborhood. We have also analysed the
halo B-type star, PG0832+676. We find [Fe/H] = -0.88 +/- 0.10, and the absence
of the B III line gives the upper limit [B/H]<2.5. These and other published
abundances are used to infer the star's evolutionary status as a post-AGB star.Comment: 31 pages, 14 figures. accepted to Ap
An Empirical Ultraviolet Template for Iron Emission in Quasars as Derived from I Zw 1
We present an empirical template spectrum suitable for fitting/subtracting
and studying the FeII and FeIII line emission in the restframe UV spectra of
active galatic nuclei (AGNs), the first empirical UV iron template to cover the
full 1250 - 3090 A range. Iron emission is often a severe contaminant in
optical--UV spectra of AGNs. Its presence complicates and limits the accuracy
of measurements of both strong and weak emission lines and the continuum
emission, affecting studies of line and continuum interrelations, the
ionization structure, and elemental abundances in AGNs. Despite the wealth of
work on modeling the AGN FeII emission and the need to account for it in
observed AGN spectra, there is no UV template electronically available to aid
this process. The iron template we present is based on HST spectra of the
Narrow Line Seyfert 1, IZw1. Its intrinsic narrow lines (~900 km/s) and rich
iron spectrum make the template particularly suitable for use with most AGN
spectra. The iron emission spectrum and the line identifications and
measurements are presented and compared with the work of Laor et al. We
illustrate the application of the derived FeII and FeIII templates by fitting
and subtracting the iron emission from UV spectra of four high-z quasars and of
the nearby quasar, 3C273. We briefly discuss the small discrepancies between
this observed iron emission and the UV template, and compare the template with
previously published ones. We discuss the advantages and limitations of the
templates and of the template fitting method. We conclude that the templates
work sufficiently well to be a valuable and important tool for eliminating and
studying the iron emission in AGNs, at least until accurate theoretical iron
emission models are developed. (Abridged)Comment: 73 pages including 7 figures, 6 tables. To appear in ApJS. Preprint
is also available at
http://www.astronomy.ohio-state.edu/~vester/IronEmission
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