Airway basal cells from human-induced pluripotent stem cells: a new frontier in cystic fibrosis research

Human-induced airway basal cells (hiBCs) derived from human-induced pluripotent stem cells (hiPSCs) offer a promising cell model for studying lung diseases, regenerative medicine, and developing new gene therapy methods. We analyzed existing differentiation protocols and proposed our own protocol for obtaining hiBCs, which involves step-by-step differentiation of hiPSCs into definitive endoderm, anterior foregut endoderm, NKX2.1+ lung progenitors, and cultivation on basal cell medium with subsequent cell sorting using the surface marker CD271 (NGFR). We derived hiBCs from two healthy cell lines and three cell lines with cystic fibrosis (CF). The obtained hiBCs, expressing basal cell markers (NGFR, KRT5, and TP63), could differentiate into lung organoids (LOs). We demonstrated that LOs derived from hiBCs can assess cystic fibrosis transmembrane conductance regulator (CFTR) channel function using the forskolin-induced swelling (FIS) assay. We also carried out non-viral (electroporation) and viral (recombinant adeno-associated virus (rAAV)) serotypes 6 and 9 and recombinant adenovirus (rAdV) serotype 5 transgene delivery to hiBCs and showed that rAAV serotype 6 is most effective against hiBCs, potentially applicable for gene therapy research

Thermal modification of wood and a complex study of its properties by magnetic resonance and other methods

© 2016, Springer-Verlag Berlin Heidelberg.Thermal modification of wood is an effective method to improve some of the properties of wood. It is reported on studies of vacuum thermal-treated wood species by magnetic resonance methods. Wood species such as Scots pine (Pinus sylvestris), birch (Betula pendula), Russian larch (Larix sibirica), Norway spruce (Picea abies), small-leaved lime (Tilia cordata) were vacuum treated by heat at 220 °C with various durations up to 8 h. This selection of wood species was investigated by electron paramagnetic resonance, nuclear magnetic resonance and microscopy methods before and after the thermal treatment. Electron paramagnetic resonance experiments revealed changes in the amount of free radicals in samples with the thermal treatment duration. Additional information on magnetic relaxation of 1H nuclei in samples at room temperature was obtained. Optical microscope analysis helped to detect structural changes in the thermally modified wood. Important properties of wood such as wood hardness and humidity absorption were also studied. The original results that were obtained correlate and complement each other, and clarify changes in the wood structure that appear with the heat treatment

Synthesis and study of the magnetic properties of nanosized and micrisized crystalline DyF₃ powders: report of philosophy Doctor thesis (translated from Russian): specialization 01.04.07 - condensed matter physics

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Pituitary Dysfunction After Aneurysmal Subarachnoid Hemorrhage: A Prospective Cohort Study

The prevalence of pituitary dysfunction after aneurysmal subarachnoid hemorrhage (aSAH) remains incompletely elucidated. Furthermore, it is not clear whether these abnormalities impact patient outcomes. The aim of this study was to evaluate the prevalence of pituitary dysfunction after aSAH and its effect on outcomes

Combination of 3‑<i>O</i>‑Levulinoyl and 6‑<i>O</i>‑Trifluorobenzoyl Groups Ensures α‑Selectivity in Glucosylations: Synthesis of the Oligosaccharides Related to <i>Aspergillus fumigatus</i> α‑(1 → 3)‑d‑Glucan

Stereospecific α-glucosylation of primary and secondary OH-group at carbohydrate acceptors is achieved using glucosyl N-phenyl-trifluoroacetimidate (PTFAI) donor protected with an electron-withdrawing 2,4,5-trifluorobenzoyl (TFB) group at O-6 and the participating levulinoyl (Lev) group at O-3. New factors have been revealed that might explain α-stereoselectivity in the case of TFB and pentafluorobenzoyl (PFB) groups at O-6. They are of conformational nature and confirmed by DFT calculations. The potential of this donor, as well as the orthogonality of TFB and Lev protecting groups, is showcased by the synthesis of α-(1 → 3)-linked pentaglucoside corresponding to Aspergillus fumigatus α-(1 → 3)-d-glucan and of its hexasaccharide derivative, bearing β-glucosamine residue at the non-reducing end

The Issue of Obedience in the Anglican Confessional Thought in the mid 1629s

Тема послушания стала актуальна для англиканской конфессио- нальной мысли после Реформации. Эта проблематика вновь начала обсуждаться в 1620-х годах, когда королевская власть попыталась собрать не легитимный, по мнению многих подданных, принудительный заем. В 1627 г. изрядное количество дворян отказалось раскошеливаться и угодило в тюрьму. Следствием стало из- вестное дело «пяти рыцарей», дебаты в парламенте 1628 г. и принятая палатами знаменитая «Петиция о праве». В конце сентября 1626 г. Карл I отправил письмо епископам, призывая их обратиться с церковных кафедр к прихожанам с просьбой «помочь и поддержать» личными средствами «защиту королевства». Набирающий политический вес, лидер арминианского крыла в церкви епископ Бата и Уэльса Уильям Лод адресовал духовенству инструкцию, призывающую «напомнить под- данным о долге послушания». В статье разбираются четыре проповеди: первая именовалась «Апостольское послушание» и была произнесена 22 февраля 1627 г. в Нортгемптоне Робертом Сибторпом, вторая, названная в печати просто «Про- поведь», принадлежит капеллану короля, настоятелю Кентерберийского собора Исааку Баргрейву и была произнесена в присутствии монарха 27 марта 1627 г., еще две под общим названием «Религия и верность» прозвучали 4 июля 1627 г. перед Карлом I в королевском дворце в Отланде, графство Сари, и 29 июля в Олдертоне, графство Сэффолк из уст другого королевского капеллана Роджера Мэнверинга. Все проповеди для большего эффекта были по повелению короля опубликованы. Англиканский клир интересовался проблемой послушания и продвигал ее среди прихожан в интерпретации, угодной королевской власти, а именно: как важнейший духовный принцип, на котором держится установленный порядок. Англиканское духовенство считало монарха божьим наместником на земле, а неповиновение королю склонно было расценивать как сопротивление самому Господу, как по- пытку разрушить устроенное им иерархическое системное единство. Проповеди показывают, что англиканские клирики были близки к тому, чтобы поставить повиновение монарху выше повиновения закону и традиции. Некоторые из них полагали, что миссия поддерживать порядок дает королю право по своему усмо- трению назначать и собирать денежные субсидии с подданных. Однако были и те, кто расценивал такие заявления, как недопустимую попытку расширить границы королевской прерогативы и как покушения на исконные вольности . The idea of obedience emerged full blown for the Anglican confessional thought after the Reformation. This problem came under discussion again in 1620s, when the royal power tried to collect non-legitimate in the eyes of many subjects forced loans. In 1627 a number of noblemen refused to dip into their purse and were imprisoned. It resulted in famous Five Knights’ case, parliament debates in 1628, which contributed to the enactment of the Petition of Right. In late September 1626 Charles I sent a letter to his bishops, demanding to appeal from their cathedrae to the parish with a request to “help and support protection of the royalty” by their personal means. Coming into political force leader of the Arminian church wing bishop of Bath and Wells William Laud addressed an instruction to the clergy, which demanded to “remind subjects about their debt of obedience”. The present paper studies four sermons: the first was called Apostolic Obedience and was delivered by Robert Sibthorpe on the 22nd of February, 1627 in Northampton, the second, simply called in press The Sermon belonged to the king’s chaplain, Dean of Canterbury Isaac Bargrave and was delivered before the king’ majesty on the 27th of March, 1627. Two sermons under common name “Religion and Allegiance” were preached before Charles I by the king’s chaplain Roger Maynwaring the one on the 4th of July 1627 at the king’s palace at Oatlands Surrey, the other on the 29th of July at Alderton Suffolk. To heighten an effect all sermons were published by order of his majesty. The Anglican clergy was interested in the problem of obedience and promoted it among the parish in interpretation acceptable before the royal power, namely as the most impotent religious principle, lying in the ground of the stated order. The Anglican Church considered the king to be the vicar of God on earth, thus disobedience to the king was judged as rebellion to Christ Himself, i.e. an attempt to destroy a hierarchically systemized community settled by Him. The sermons show that the Anglican clergy was close to place obedience to the monarch higher than obedience to law or tradition. Some of them believed that a mission to support the order gave the king a right to set and collect monetary contributions from his subjects to his own discretion. Nonetheless, the others regarded such claims as an intolerable attempt to broaden the limits of royal prerogative and as aggression upon ancient liberties of subjects

Pseudorapidity densities of charged particles with transverse momentum thresholds in pp collisions at √ s = 5.02 and 13 TeV

The pseudorapidity density of charged particles with minimum transverse momentum (pT) thresholds of 0.15, 0.5, 1, and 2 GeV/c is measured in pp collisions at the center of mass energies of √s=5.02 and 13 TeV with the ALICE detector. The study is carried out for inelastic collisions with at least one primary charged particle having a pseudorapidity (η) within 0.8pT larger than the corresponding threshold. In addition, measurements without pT-thresholds are performed for inelastic and nonsingle-diffractive events as well as for inelastic events with at least one charged particle having |η|2GeV/c), highlighting the importance of such measurements for tuning event generators. The new measurements agree within uncertainties with results from the ATLAS and CMS experiments obtained at √s=13TeV.