1,401 research outputs found
Separation and quantification of alkylphosphocholines by reversed phase high performance liquid chromatography
Sequential epiretinal membrane removal with internal limiting membrane peeling in brilliant blue G-assisted macular surgery
Purpose
To assess the selectivity of brilliant blue G (BBG) staining by analysing the morphological components of unstained and stained tissue obtained during epiretinal membrane (ERM) removal with internal limiting membrane (ILM) peeling in BBG-assisted macular surgery.
Methods
Twenty-six surgical specimens were removed from 13 eyes with epiretinal gliosis during vitrectomy using BBG for ERM and ILM peeling. We included eyes with idiopathic macular pucker, idiopathic macular hole and vitreomacular traction syndrome. The dye was injected into the fluid-filled globe. Unstained and stained epiretinal tissue was harvested consecutively and placed into separate containers. All specimens were processed for conventional transmission electron microscopy.
Results
The first surgical specimen of all eyes showed no intraoperative staining with BBG and corresponded to masses of cells and collagen. The second surgical specimen demonstrated good staining characteristics and corresponded to the ILM in all patients included. In seven eyes, the ILM specimens were seen with minor cell proliferations such as single cells or a monolayer of cells. Myofibroblasts, fibroblasts and astrocytes were present. In five cases, native vitreous collagen fibrils were found at the ILM. In six of the eyes, ILM specimens were blank.
Conclusion
Our clinicopathological correlation underlines the selective staining properties of BBG. The residual ILM is selectively stained by BBG even when a small amount of cells and collagen adheres to its vitreal side. To reduce the retinal exposure to the dye, the surgeon might choose to remove the ERM without using the dye, followed by a BBG injection to identify residual ILM
Synthesis and use of a lysolecithin analog for the purification of UDP-glucuronosyltransferase.
Conformation of hexadecylphosphocholine, an anticancer drug, by molecular dynamics and NMR methods
Decoherence-Free Quantum Information Processing with Four-Photon Entangled States
Decoherence-free states protect quantum information from collective noise,
the predominant cause of decoherence in current implementations of quantum
communication and computation. Here we demonstrate that spontaneous parametric
down-conversion can be used to generate four-photon states which enable the
encoding of one qubit in a decoherence-free subspace. The immunity against
noise is verified by quantum state tomography of the encoded qubit. We show
that particular states of the encoded qubit can be distinguished by local
measurements on the four photons only.Comment: 4 pages, 4 eps figures, revtex
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The effect of relationship status on communicating emotions through touch
Research into emotional communication to date has largely focused on facial and vocal expressions. In contrast, recent studies by Hertenstein, Keltner, App, Bulleit, and Jaskolka (2006) and Hertenstein, Holmes, McCullough, and Keltner (2009) exploring nonverbal communication of emotion discovered that people could identify anger, disgust, fear, gratitude, happiness, love, sadness and sympathy from the experience of being touched on either the arm or body by a stranger, without seeing the touch. The study showed that strangers were unable to communicate the self-focused emotions embarrassment, envy and pride, or the universal emotion surprise. Literature relating to touch indicates that the interpretation of a tactile experience is significantly influenced by the relationship between the touchers (Coan, Schaefer, & Davidson, 2006). The present study compared the ability of romantic couples and strangers to communicate emotions solely via touch. Results showed that both strangers and romantic couples were able to communicate universal and prosocial emotions, whereas only romantic couples were able to communicate the self-focused emotions envy and pride
Micrometre-scale deformation observations reveal fundamental controls on geological rifting
Many of the world’s largest volcanic eruptions are associated with geological rifting where major fractures open at the Earth’s surface, yet fundamental controls on the near-surface response to the rifting process are lacking. New high resolution observations gleaned from seismometer data during the 2014 Bárðarbunga basaltic dyke intrusion in Iceland allow us unprecedented access to the associated graben formation process on both sub-second and micrometre scales. We find that what appears as quasi steady-state near-surface rifting on lower resolution GPS observation comprises discrete staccatolike deformation steps as the upper crust unzips through repetitive low magnitude (MW < 0) failures on fracture patches estimated between 300 m2 and 1200 m2 in size. Stress drops for these events are one to two orders of magnitude smaller than expected for tectonic earthquakes, demonstrating that the uppermost crust in the rift zone is exceptionally weak
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