Tissue eosinophilia induced by recombinant human interleukin-5 in the hamster cheek pouch membrane

Interleukin-5 (IL-5) is a cytokine that preferentially effects the development and function of eosinophils, and is considered important in the pathophysiology of allergic inflammation. In this study, we evaluated the ability of recombinant human IL-5 (rHu IL-5) to promote tissue eosinophilia and the importance of this eosinophilia to pathological alterations in vascular function. Repetitive subcutaneous administration for 18 days of rHu IL-5 resulted in a 7-fold increase in the number of eosinophils found in the ipsilateral hamster cheek pouch membrane. The contralateral cheek pouch membrane and peritoneum of these animals showed lesser but significant elevations in the number of eosinophils. In contrast, denatured rHu IL-5 did not elevate eosinophils in these tissues. Through the use of intravital microscopy and fluorometric analysis, rHu IL-5 treated hamster cheek pouch membranes were evaluated for alterations in microvascular permeability, using plasma clearance of FITC-dextran 150 as an index. Despite promoting a prominent tissue eosinophilia, the repetitive subcutaneous injections of rHu IL-5 did not alter the clearance of FITC-dextran 150. Topical application of rHu IL-5 to the cheek pouch, also, had no effect on the clearance of FITC-dextran 150. Immunofluorescence observations using an antibody to the granule protein, eosinophil peroxidase, indicated that the recruited cells had not degranulated. Our results support the importance of IL-5 in the recruitment of tissue eosinophils, but further stimulation is probably required to cause degranulation of these cells and the ensuing tissue damage

A collaboration to improve perioperative acute pain care at the University Teaching Hospital of Butare, Rwanda

BACKGROUND: A perioperative acute pain care program integrating standardized assessment and treatment forms into pain care was developed and implemented at an urban hospital in Rwanda through a collaboration between Rwandan and Canadian experts. This study evaluated the perioperative acute pain care program using a quality improvement lens. METHODS: Using the Model for Improvement: Plan, Do, Study, Act (PDSA) cycle, a mixed methods evaluation was performed. Over one year, 519 randomized patient chart audits were conducted and analyzed through control charts. Through purposeful sampling, focus groups comprised ofsurgeons and nurses (N=34) involved in pain care in surgery, obstetrics, and anesthesiology were performed and analyzed via thematic coding. RESULTS: The average attempted form completion rate across all forms varied monthly between 56-93% (mean=79%; median=81%). Across all forms, both the mean and median total number of errors per form were 12.5. Enablers of form use included improved pain care for patients and feelings of professional satisfaction. Program implementation was challenged by resource constraints, form integration, and health care provider training. CONCLUSION: Future quality improvement collaborations should identify and address improved pain care while working with local experts to ensure PDSA cycles are continuous, and evidence based

Low-Frequency Radio Transients in the Galactic Center

We report the detection of a new radio transient source, GCRT J1746-2757, located only 1.1 degrees north of the Galactic center. Consistent with other radio transients toward the Galactic center, this source brightened and faded on a time scale of a few months. No X-ray counterpart was detected. We also report new 0.33 GHz measurements of the radio counterpart to the X-ray transient source, XTE J1748-288, previously detected and monitored at higher radio frequencies. We show that the spectrum of XTE J1748-288 steepened considerably during a period of a few months after its peak. We also discuss the need for a more efficient means of finding additional radio transients

Searching for dark clouds in the outer galactic plane I -- A statistical approach for identifying extended red(dened) regions in 2MASS

[Abridged] Though the exact role of infrared dark clouds in the formation process is still somewhat unclear, they seem to provide useful laboratories to study the very early stages of clustered star formation. Infrared dark clouds have been identified predominantly toward the bright inner parts of the galactic plane. The low background emission makes it more difficult to identify similar objects in mid-infrared absorption in the outer parts. This is unfortunate, because the outer Galaxy represents the only nearby region where we can study effects of different (external) conditions on the star formation process. The aim of this paper is to identify extended red regions in the outer galactic plane based on reddening of stars in the near-infrared. We argue that these regions appear reddened mainly due to extinction caused by molecular clouds and young stellar objects. The work presented here is used as a basis for identifying star forming regions and in particular the very early stages. We use the Mann-Whitney U-test, in combination with a friends-of-friends algorithm, to identify extended reddened regions in the 2MASS all-sky JHK survey. We process the data on a regular grid using two different resolutions, 60" and 90". The two resolutions have been chosen because the stellar surface density varies between the crowded spiral arm regions and the sparsely populated galactic anti-center region. We identify 1320 extended red regions at the higher resolution and 1589 at the lower resolution run. The majority of regions are associated with major molecular cloud complexes, supporting our hypothesis that the reddening is mostly due to foreground clouds and embedded objects.Comment: Accepted for publication in A&A -- 9 pages, 5 figures (+ on-line only tables

Spitzer's mid-infrared view on an outer Galaxy Infrared Dark Cloud candidate toward NGC 7538

Infrared Dark Clouds (IRDCs) represent the earliest observed stages of clustered star formation, characterized by large column densities of cold and dense molecular material observed in silhouette against a bright background of mid-IR emission. Up to now, IRDCs were predominantly known toward the inner Galaxy where background infrared emission levels are high. We present Spitzer observations with the Infrared Camera Array toward object G111.80+0.58 (G111) in the outer Galactic Plane, located at a distance of ~3 kpc from us and ~10 kpc from the Galactic center. Earlier results show that G111 is a massive, cold molecular clump very similar to IRDCs. The mid-IR Spitzer observations unambiguously detect object G111 in absorption. We have identified for the first time an IRDC in the outer Galaxy, which confirms the suggestion that cluster-forming clumps are present throughout the Galactic Plane. However, against a low mid-IR back ground such as the outer Galaxy it takes some effort to find them.Comment: Accepted for publication in ApJL -- 11 pages, 2 figures (1 colour

A massive cluster of Red Supergiants at the base of the Scutum-Crux arm

We report on the unprecedented Red Supergiant (RSG) population of a massive young cluster, located at the base of the Scutum-Crux Galactic arm. We identify candidate cluster RSGs based on {\it 2MASS} photometry and medium resolution spectroscopy. With follow-up high-resolution spectroscopy, we use CO-bandhead equivalent width and high-precision radial velocity measurements to identify a core grouping of 26 physically-associated RSGs -- the largest such cluster known to-date. Using the stars' velocity dispersion, and their inferred luminosities in conjuction with evolutionary models, we argue that the cluster has an initial mass of $\sim$40,000\msun, and is therefore among the most massive in the galaxy. Further, the cluster is only a few hundred parsecs away from the cluster of 14 RSGs recently reported by Figer et al (2006). These two RSG clusters represent 20% of all known RSGs in the Galaxy, and now offer the unique opportunity to study the pre-supernova evolution of massive stars, and the Blue- to Red-Supergiant ratio at uniform metallicity. We use GLIMPSE, MIPSGAL and MAGPIS survey data to identify several objects in the field of the larger cluster which seem to be indicative of recent region-wide starburst activity at the point where the Scutum-Crux arm intercepts the Galactic bulge. Future abundance studies of these clusters will therefore permit the study of the chemical evolution and metallicity gradient of the Galaxy in the region where the disk meets the bulge.Comment: 49 pages, 22 figures. Accepted for publication in ApJ. Version with hi-res figures can be found at http://www.cis.rit.edu/~bxdpci/RSGC2.pd

Phase field modeling of electrochemistry I: Equilibrium

A diffuse interface (phase field) model for an electrochemical system is developed. We describe the minimal set of components needed to model an electrochemical interface and present a variational derivation of the governing equations. With a simple set of assumptions: mass and volume constraints, Poisson's equation, ideal solution thermodynamics in the bulk, and a simple description of the competing energies in the interface, the model captures the charge separation associated with the equilibrium double layer at the electrochemical interface. The decay of the electrostatic potential in the electrolyte agrees with the classical Gouy-Chapman and Debye-H\"uckel theories. We calculate the surface energy, surface charge, and differential capacitance as functions of potential and find qualitative agreement between the model and existing theories and experiments. In particular, the differential capacitance curves exhibit complex shapes with multiple extrema, as exhibited in many electrochemical systems.Comment: v3: To be published in Phys. Rev. E v2: Added link to cond-mat/0308179 in References 13 pages, 6 figures in 15 files, REVTeX 4, SIUnits.sty. Precedes cond-mat/030817

Leading together, learning together: music education and music therapy students' perceptions of a shared practicum

The health benefits of musical engagement extend across the lifespan, with research documenting developmental and quality of life outcomes in senior adulthood. Whilst the psychological functions of music include three broad domains: cognitive, emotional and social, the social factors of music consumption have been, for the most part, ignored. This project is predicated on the understanding that students "construct" their own knowledge through engagement with others. It also reflects the belief that it is possible to link the world of learning with a world of action through a reflective process with small cooperative learning groups.This article reports on a collaborative creativity leadership project implemented by music therapy and music education students. In particular, it explores the perceptions of the students as they employed a teaching/therapeutic intervention with ageing participants from a local private retirement village. The project involved lyric rewriting and singing performance. Drawing on the students' reflective journals and interview transcripts, four themes emerged relating to students' perceptions of the learning experience. These themes were: learning about facilitating, learning about self, learning about the musical process, and learning about the context/ageing. Each of these themes is explored before conclusions about the process of leading a creative collaboration are drawn. The implications of this analysis extend beyond the context of a retirement village, and into various educational and therapeutic contexts where creative collaboration is facilitated

Rethinking globalised resistance : feminist activism and critical theorising in international relations

This article argues that a feminist approach to the 'politics of resistance' offers a number of important empirical insights which, in turn, open up lines of theoretical inquiry which critical theorists in IR would do well to explore. Concretely, we draw on our ongoing research into feminist 'anti-globalisation' activism to rethink the nature of the subject of the politics of resistance, the conditions under which resistance emerges and how resistance is enacted and expressed. We begin by discussing the relationship of feminism to critical IR theory as a way of situating and explaining the focus and approach of our research project. We then summarise our key empirical arguments regarding the emergence, structure, beliefs, identities and practices of feminist 'anti-globalisation' activism before exploring the implications of these for a renewed critical theoretical agenda in IR