15 research outputs found

    Applying UX/UI design in website creation

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    The paper presents the results of the study on the application of the principles of UX/UI design in ensuring the functionality of the website of interior decorations and household accessories. The layout of the site and the responsive design of an online store and the evaluation of the results using web analysis tools were made. The current principles in the field of UX/UI design and their use for the process of developing and promoting websites have been studied

    THE EFFECT OF ZnO NANOPARTICLES ON THE ACTIVITY OF ANTIOXIDANT ENZYMES AND CAROTENOID CONTENT AT RHODOSPORIDIUM TORULOIDES CNMN-Y-30 YEAST

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    The present research paper provides new information on the influence of ZnO nanoparticles (ZnO NPs) of size <50 nm and <100 nm on Rhodosporidium toruloides CNMN-Y-30 pigmented yeast. It was established that the activity of antioxidant enzymes such as catalase, superoxide dismutase and content of carotenoid pigments in the studied strain has been modified depending on the size and concentrations of NPs. There were no significant differences between the activity of antioxidant enzymes and content of carotenoid pigments in experimental group and control at the use of significantly low concentration of ZnO NPs. The use of nanoparticles in concentration of 30 mg/l caused a decrease in activity of antioxidant enzyme catalase and contributed to the increase in the activity of superoxide dismutase. This study has revealed that the concentration of 30 mg/L of ZnO NPs initiates an significant decrease in the content of carotenoid pigments - β-carotene, torulene and torularhodin in cell biomass. The results provided opportunities for modeling cell cycle processes and highlighting of carotenoid pigments and antioxidant enzymes as parameters for determining the mode of action of nanoparticles. &nbsp

    Biosynthesis of β-glucans and morphological features Saccharomyces cerevisiae CNMN-Y-20 yeasts under the action of zinc oxide nanoparticles

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    The paper provides new information on β-glucans biosynthesis capacity and the morphological features modification of cells and colonies of Saccharomyces cerevisiae CNMN-Y-20 yeast strain known as producer of β-glucans. It was found that the development cycle and bioproductive capacity of the yeast was affected by cultivation in the presence of ZnO in a concentrations and the contact duration manner. Within 6-24 hours, the reproduction of the nanomodified cell was decreased compared to the control, but after 120 hours of submerged cultivation an insignificant increase in biomass content relative to 1L culture medium was recorded predominantly related to nanoparticle concentrations 0.5-1.0 mg/L. ZnO nanoparticles (<100 nm). Concentrations of 0.5-15 mg/L initiated a 13-15% increase in average dimensions of Saccharomyces cerevisiae CNMN-Y-20 cells and 7-12% increase in the ability to form of β-glucans, especially at concentrations of 1 and 5 mg/L. The correlation coefficient between the cells area and the β-glucans amount is strong (R² = 0.8021). The results provide the possibility of enhancing of the range of analyzes and the formation of reference bases necessary for the strategy to enlarge the biotechnological performance of yeasts

    Evaluation of the biochemical composition and antioxidant activity of preparation based on pigments extracted from the remaining biomass of <i>Arthrospira platensis</i>

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    Biotechnological research is currently focused on obtaining preparations based on natural pigments due to their properties and positive impact on human and animal health. Thus, this study aimed to evaluate the biochemical composition and antioxidant activity of the preparation based on pigments obtained from the remaining biomass of Arthrospira platensis. The obtained results established that the preparation is characterized by a high content of β-carotene, lutein, chlorophyll pigments, and sulfated polysaccharides. Due to its composition, the preparation also possesses high antioxidant activity and the catalase and superoxide dismutase enzymes. These findings highlight the high biological value of the new preparation and the enormous potential for implementation in medicine, the animal husbandry sector, and the food and cosmetic industry

    The Effects of ZnO Nanoparticles in Combination with Alcohol on Biosynthetic Potential of Saccharomyces cerevisiae

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    This paper reports about experimental results concerning the influence of 30 nm ZnO nanoparticles on biomass, carbohydrates, β-glucans, proteins accumulation and catalase enzyme activity at Saccharomyces cerevisiae CNMN-Y-20 yeast strain exposed to alcohol action. Alcohol in concentrations of 2%, 5% and 10% added to culture medium has been reported to stimulate β-glucans biosynthesis and to inhibit protein synthesis. Low biomass production, with 71% less that control, was detected in the experiments with 10% alcohol. ZnO nanoparticles in combination with alcohol do not offer sufficient protection for the proteins biosynthesis, but efficiently protect the carbohydrates and β-glucans biosynthetic processes, which contents in the biomass are with 16.6% and 19.9% higher than control, respectively. The maximum value of β-glucans content was established in case of cultivation of selected yeast strain on YPD medium supplemented with 5 mg/L nanoparticles ZnO and 2% alcohol. The obtained results allowed the elaboration of new procedure for directed synthesis of β-glucans that contributed to an increase of this component with 30.7%, compared to control

    The evaluation of nanoparticles ZnO and TiO2 effects on Saccharomyces cerevisiae CNMN-Y-20 yeast strain

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    This paper investigates the action of nanoparticles ZnO (10 nm) and TiO2 (30 nm) on growth of Saccharomyces cerevisiae CNMN-Y-20 yeast. Nanoparticles in concentration of 0,5; 1,0 and 5,0 mg/L in YPD medium did not modify significantly cell proliferation, biomass production, the carbohydrate content and the content of β-glucans at Saccharomyces cerevisiae CNMN-Y-20. Nanoparticles ZnO and TiO2 contributed to the decrease in protein content, which demonstrated the appearance of the alterations of yeast cell membranes

    Effect of Fe3O4 and TiO2 Nanoparticles on Catalase Activity and β-Carotene Content at Pigmented Yeast Strain Rhodotorula gracilis

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    The properties of nanoparticles have been used in a wide range of potential applications in food industry, medicine, microbial biotechnology, cosmetics, environmental production. Research results of evaluation of the effect of Fe3O4 and TiO2 nanoparticles applicated in large concentration limits on some parameters of pigmented yeast strain Rhodotorula gracilis CNMN-Y-30 are presented in this paper. It was established that nanoparticles selected for study caused toxic effects on β-caroten accumulation and activity of antioxidant enzyme catalase depending on concentration and nanostructure. The strong correlation between concentration of nanoparticles and evaluated components has been revealed at studied yeast strain. Coefficients of correlation varied in limits R2 = 0.623... 0.951. For the first time, the determination of β-carotene accumulation and catalase activity rate as functional tests for estimation of toxicity of nanoparticles for pigmented yeast strain Rhodotorula gracilis was effectuated. Thus, it was established that adaptive response of yeast strain to the presence of Fe3O4 and TiO2 nanoparticles has been manifested by modification of the processes of β-carotene biosythesis and catalase activity

    The Using of Millimeter Waves for Biosynthetic Processes Stimulation in Saccharomyces Cerevisiae

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    The results of influence of three frequencies of electromagnetic radiation of highfrequency range (EMR EHF) on the biosynthesis of carbohydrates, β-glucan, proteins, catalase activity by Saccharomyces cerevisiae CNMN -Y-20 yeast strain were analysed. It was established that frequency of f= 53,33 GHz stimulates the biosynthesis of carbohydrates, including β-glucan and frequency of f= 42,19 GHz promotes the increase of protein content and catalase. The indicated frequencies of EMR EHF are offered for the use in the biotechnology of cultivation of yeasts with the purpose to increase biosynthetic properties of yeast strai
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