Individual variability of progeny survival rates at different stages from females fed the C and the V diet (in bold).

<p>Individual variability of progeny survival rates at different stages from females fed the C and the V diet (in bold).</p

Biometric parameters, reproduction performance and survival rates.

<p>Data are presented as mean ± SD. <i>p-values</i> were produced by independent sample t-test. <i>ns</i>: not significant</p><p>Biometric parameters, reproduction performance and survival rates.</p

Fatty acid composition (% of total FA) of ova and swim-up fry (polar and neutral lipid fractions).

<p>Data are presented as mean ± SD. <i>p-values</i> were produced by independent sample t-test or equivalent non-parametric test (two-sample Wilcoxon test)</p><p>MUFA: monounsaturated fatty acids</p><p><i>ns</i>: not significant</p><p>Fatty acid composition (% of total FA) of ova and swim-up fry (polar and neutral lipid fractions).</p

Individual variability of progeny survival rates at different stages from females fed the C and the V diet (in bold).

<p>Individual variability of progeny survival rates at different stages from females fed the C and the V diet (in bold).</p

Fatty acid composition (% of total fatty acids) of diets.

<p>C _1–2: commercial diet <i>Le Gouessant</i>, V: experimental 100% plant-based diet</p><p>C₁: fed until year-2 spawning; C₂: fed between year-2 and year-3 spawning</p><p>MUFA: monounsaturated fatty acids</p><p><i>nd</i>: not detected</p><p>Fatty acid composition (% of total fatty acids) of diets.</p

Total lipid content in female tissues, ova and swim-up fry.

<p>Data are presented as mean ± SD. p-values were produced by independent sample t-test. Different letters indicates significant differences (<i>p-value</i> <0.05).</p

Fatty acid composition (% of total FA) of livers, carcasses and digestive tracts (polar and neutral lipid fractions).

<p>Data are presented as mean ± SD; <i>p-values</i> were produced by independent sample t-test or equivalent non-parametric test (two-sample Wilcoxon test)</p><p>MUFA: monounsaturated fatty acids</p><p><i>nc</i>: <i>p-value</i> not produced, PL for the V group pooled for practical reasons</p><p>Fatty acid composition (% of total FA) of livers, carcasses and digestive tracts (polar and neutral lipid fractions).</p

Ingredients and composition of diets.

<p>*Origin co-fishery products—all species</p><p>** Origin co-fishery products—sardines</p><p>C: commercial diet <i>Le Gouessant</i>, V: experimental 100% plant-based diet</p><p>Ingredients and composition of diets.</p

ARA, EPA and DHA content (g/100g ova) in ova from second- and third-year spawning.

<p>Data are presented as mean ±SD. <i>p-values</i> were produced by independent sample t-test</p><p><i>ns</i>: not significant</p><p>ARA, EPA and DHA content (g/100g ova) in ova from second- and third-year spawning.</p

Early IFN- dependent and independent viral inhibition in B57 cells.

<p>(A): The expression of shIFNφ1 transcript in A22 and B57 cells after VHSV infection was measured by qPCR 4, 8 and 24 hours post infection by VHSV or in mock infected cells (ctrl). mRNA copy numbers were normalized on ß-actin expression (measured ratio of shIFNφ1 mRNA/actin mRNA). The mean of three experiments is shown. (B): Expression of N viral gene in cells after VHSV 07-71 infection at different time. Expression of gene is measured by qPCR after 4, 8 and 24 hours of infection by VHSV 07-71 or without viral infection (ctrl). Gene expression is evaluated relative to ß-actin expression (measured ratio of VHSV N mRNA/actin mRNA). The mean of three experiments is shown. (C): Viral genomic RNA (strand+ plus strand−) was quantified using qPCR. The virus replication was assessed by the ratio of virus genome at 6 h versus 2 h post-inoculation. (D): The mRNA encoding the viral N protein was quantified in parallel and the ratio N mRNA 6 hours post inoculation/N mRNA 2 hours post inoculation is represented.</p