Moire fringe measurements of corneal analogues

Moire fringe measurements of corneal analogue

The Conscience of Macbeth

Whatever are the other merits of Macbeth, it must be classed as one of the most penetrating studies of conscience in literature. Shakespeare does not attempt to describe in the drama how the ordinary criminal would react to evil, but how Shakespeare himself would have felt if he had fallen into crime. 1 The ramifications of this conflict between the conscience of a man of genius and the supernatural forces of wickedness, therefore, assume immense dimensions. "Macbeth leaves on most readers a profound impression of the misery of a guilty conscience and the retribution of crime . . . But what Shakespeare perhaps felt even more deeply, when he wrote this play, was the incalculability of evil--that in meddling with it human beings do they know not what."2 This drama displays an evil not to be accounted for simply in terms of the protagonist's will or his causal relationships to evil. It is an agency which is beyond the power of Macbeth's will; and his conscience, as powerful and imaginative as it is, can only warn him that he is involving himself in a force which will cause him unexpected and hideous mental pain. If there is a moral in Macbeth, it is obviously that men should not tamper with evil, for not even a deep-rooted conscience and an ascendant will can contend with its influence

Validating a Vegetative Filter Strip Performance Model

Vegetative filter strips (VFS) reduce losses of nutrients, solids, and other materials from land area treated with fertilizers and manures. A number of models are available that simulate nutrient and sediment transport in VFS. While VFS effectiveness is considered to depend on lengths of pollutant source area and VFS areas, few published studies have tried to validate these models using variable pollutant source area and VFS area. The objective of this study was to validate an event-based nutrient transport model (Chaubey et al., 1995) that simulates soluble nutrient transport in VFS. This model links three sub-models: modified Green-Ampt infiltration, non-linear kinematic wave overland flow routing, and a nutrient transport component. The nutrient transport component considers infiltration as the only mechanism of pollutant removal from runoff. Data from a field plot experiment were used to validate the model. The model was executed using an uncalibrated runoff component, a calibrated runoff component, and measured runoff. The concentrations of parameters entering the VFS from three different poultry litter application lengths (6.1, 12.2, and 18.3 m) were not significantly different. However, predicted concentrations at subsequent lengths were different for all the three poultry litter application lengths. This finding was consistent with the observed data. Model execution with the uncalibrated runoff component, calibrated runoff component, and measured runoff underpredicted concentrations and mass transport at various locations along the length of the VFS. Underprediction of concentration was judged to be the reason for underprediction of mass transport. The agreement between the observed and predicted concentrations and mass transport, however, improved when runoff predictions from the calibrated runoff component and measured runoff were used. This suggests that accurate prediction of infiltration and runoff is critical for accurate prediction of concentration mass transport. Furthermore, since concentration was underpredicted even when measured runoff was used, this study suggests that the nutrient transport component might be improved, possibly by including nutrient removal mechanisms other than infiltration

The Crystal Structure of the Extracellular 11-heme Cytochrome UndA Reveals a Conserved 10-heme Motif and Defined Binding Site for Soluble Iron Chelates

Members of the genus Shewanella translocate deca- or undeca-heme cytochromes to the external cell surface thus enabling respiration using extracellular minerals and polynuclear Fe(III) chelates. The high resolution structure of the first undeca-heme outer membrane cytochrome, UndA, reveals a crossed heme chain with four potential electron ingress/egress sites arranged within four domains. Sequence and structural alignment of UndA and the deca-heme MtrF reveals the extra heme of UndA is inserted between MtrF hemes 6 and 7. The remaining UndA hemes can be superposed over the heme chain of the decaheme MtrF, suggesting that a ten heme core is conserved between outer membrane cytochromes. The UndA structure has also been crystallographically resolved in complex with substrates, an Fe(III)-nitrilotriacetate dimer or an Fe(III)-citrate trimer. The structural resolution of these UndA-Fe(III)-chelate complexes provides a rationale for previous kinetic measurements on UndA and other outer membrane cytochromes

Signal transduction and activator of transcription-3 (STAT3) in patients with colorectal cancer: associations with the phenotypic features of the tumour and host

Purpose: In patients with colorectal cancer (CRC), a high-density local inflammatory infiltrate response is associated with improved survival, whereas elevated systemic inflammatory responses are associated with poor survival. One potential unifying mechanism is the IL-6/JAK/STAT3 pathway. The present study examines the relationship between tumour total STAT3 and phosphorylated STAT3Tyr705 (pSTAT3) expression, host inflammatory responses and survival in patients undergoing resection of stage I-III CRC. Experimental Design: Immunohistochemical assessment of STAT3/pSTAT3 expression was performed using a tissue microarray and tumour cell expression divided into tertiles using the weighted histoscore. The relationship between STAT3/pSTAT3 expression and local inflammatory (CD3+, CD8+, CD45R0+, FOXP3+ T-cell density and Klintrup-Mäkinen grade) and systemic inflammatory responses and cancer-specific survival were examined. Results: 196 patients were included in the analysis. Cytoplasmic and nuclear STAT3 expression strongly correlated (r=0.363, P<0.001); nuclear STAT3 and pSTAT3 expression weakly correlated (r=0.130, P=0.068). Cytoplasmic STAT3 was inversely associated with the density of CD3+ (P=0.012), CD8+ (P=0.003) and FOXP3+ T-lymphocytes (P=0.002) within the cancer cell nests and was associated with an elevated systemic inflammatory response as measured by modified Glasgow Prognostic Score (mGPS2: 19% vs. 4%, P=0.004). The combination of nuclear STAT3/pSTAT3 stratified five-year survival from 81% to 62% (P=0.012), however was not associated with survival independent of venous invasion, tumour perforation or tumour budding. Conclusion In patients undergoing CRC resection, STAT3 expression was associated with adverse host inflammatory responses and reduced survival. Up-regulation of tumour STAT3 may be an important mechanism whereby the tumour deregulates local and systemic inflammatory responses

Development of Wave Energy Converters at Ocean Power Technologies (Extended Abstract)

This abstract contains information relevant to the use of wave information for naval operations, education, and alternative energy technologies, and was used, along with the Session Presentation, to facilitate discussion during Session 1 (the use of wave measurements to support operations)

Mechanism of high-mobility group protein B enhancement of progesterone receptor sequence-specific DNA binding

The DNA-binding domain (DBD) of progesterone receptor (PR) is bipartite containing a zinc module core that interacts with progesterone response elements (PRE), and a short flexible carboxyl terminal extension (CTE) that interacts with the minor groove flanking the PRE. The chromosomal high-mobility group B proteins (HMGB), defined as DNA architectural proteins capable of bending DNA, also function as auxiliary factors that increase the DNA-binding affinity of PR and other steroid receptors by mechanisms that are not well defined. Here we show that the CTE of PR contains a specific binding site for HMGB that is required for stimulation of PR-PRE binding, whereas the DNA architectural properties of HMGB are dispensable. Specific PRE DNA inhibited HMGB binding to the CTE, indicating that DNA and HMGB–CTE interactions are mutually exclusive. Exogenous CTE peptide increased PR-binding affinity for PRE as did deletion of the CTE. In a PR-binding site selection assay, A/T sequences flanking the PRE were enriched by HMGB, indicating that PR DNA-binding specificity is also altered by HMGB. We conclude that a transient HMGB–CTE interaction alters a repressive conformation of the flexible CTE enabling it to bind to preferred sequences flanking the PRE

Non-perturbative Quantum Propagators in Bounded Spaces

We outline a new approach to calculating the quantum mechanical propagator in the presence of geometrically non-trivial Dirichlet boundary conditions based upon a generalisation of an integral transform of the propagator studied in previous work (the so-called ``hit function''), and a convergent sequence of Pad\'e approximants. In this paper the generalised hit function is defined as a many-point propagator and we describe its relation to the sum over trajectories in the Feynman path integral. We then show how it can be used to calculate the Feynman propagator. We calculate analytically all such hit functions in $D=1$ and $D=3$ dimensions, giving recursion relations between them in the same or different dimensions and apply the results to the simple cases of propagation in the presence of perfectly conducting planar and spherical plates. We use these results to conjecture a general analytical formula for the propagator when Dirichlet boundary conditions are present in a given geometry, also explaining how it can be extended for application for more general, non-localised potentials. Our work has resonance with previous results obtained by Grosche in the study of path integrals in the presence of delta potentials. We indicate the eventual application in a relativistic context to determining Casimir energies using this technique.Comment: 26 pages,6 figures, 5 appendice