Advantage of high turndown SUB for intensified process

Disposable technology is being used more each year in the biotechnology industry. Disposable bioreactors allow the user to avoid expenses associated with cleaning, assembly and operations, as well as equipment validation. Disposable bioreactors have played a key role to meet the increasing run rate of the Cell Culture Pilot Plant while maintaining a high success rate, reducing labor costs, increasing efficiency, and lowering the risk of contamination. Recent effort to evaluate the next generation 10:1 turndown single use bioreactor with different cell retention devices to challenges oxygen mass transfer, carbon-dioxide stripping while assessing foaming, and vent filter sizing. This presentation will focus primarily on the advantage of the next generation high turndown single use bioreactor that comes with different sparger options (enhanced DHS and mircrosparger), and tubing for different cell retention device connection. Additionally, the high turndown provided a wide range of working volume allowing us to truly test the true scale down of the cell retention device for its maximum flux and filter throughput. The evaluation provided important system performance, operation experience, and cell culture performance data when comparing the next generation high turndown disposable system with respect to the legacy 2:1 and 5:1 turndown single use bioreactors, and conventional stainless-steel bioreactor system

Preliminary Results of Initial Testing for Coronavirus (COVID-19) in the Emergency Department

Introduction: On March 10, 2020, the World Health Organization declared a global pandemic due to widespread infection of the novel coronavirus 2019 (COVID-19). We report the preliminary results of a targeted program of COVID-19 infection testing in the ED in the first 10 days of its initiation at our institution.Methods: We conducted a review of prospectively collected data on all ED patients who had targeted testing for acute COVID-19 infection at two EDs during the initial 10 days of testing (March 10-19, 2020). During this initial period with limited resources, testing was targeted toward high-risk patients per Centers for Disease Control and Prevention guidelines. Data collected from patients who were tested included demographics, clinical characteristics, and test qualifying criteria. We present the data overall and by test results with descriptive statistics.Results: During the 10-day study period, the combined census of the study EDs was 2157 patient encounters. A total of 283 tests were ordered in the ED. The majority of patients were 18-64 years of age, male, non-Hispanic white, had an Emergency Severity Index score of three, did not have a fever, and were discharged from the ED. A total of 29 (10.2%) tested positive. Symptoms-based criteria most associated with COVID-19 were the most common criteria identified for testing (90.6%). All other criteria were reported in 5.51–43.0% of persons being tested. Having contact with a person under investigation was significantly more common in those who tested positive compared to those who tested negative (63% vs 24.5%, respectively). The majority of patients in both results groups had at least two qualifying criteria for testing (75.2%).Conclusion: In this review of prospectively collected data on all ED patients who had targeted testing for acute COVID-19 infection at two EDs in the first 10 days of testing, we found that 10.2% of those tested were identified as positive. The continued monitoring of testing and results will help providers understand how COVID-19 is progressing in the community

An Efficient Approach for Generalized Load Balancing in Multipath Packet Switched Networks

This paper is a quantitative analysis on packet switched network with a view to generalize load balancing and determination of appropriate routing algorithm in multipath environment. Several routing algorithms have been introduced for routing of packets from source to destination. Some of them route packets accurately with increased workload and some of them drastically cut down the workload. A few of them can find out a minimum workload deviation for both UDP and TCP packets. We simulated these approaches in a well defined simulator, analyzed and evaluated their performance. After expanding our analysis with varying weights and number of paths we found that the recently proposed routing algorithm Mixed Weighted Fair Routing (MWFR) outperforms the existing routing algorithms by reducing the routing and network overhead and saving the scarce bandwidth as well as CPU consumption for packet switching networks.Comment: 12 Pages, IJCNC Journal 201

Effect of neurostimulation on cognition and mood in refractory epilepsy.

Epilepsy is a common, debilitating neurological disorder characterized by recurrent seizures. Mood disorders and cognitive deficits are common comorbidities in epilepsy that, like seizures, profoundly influence quality of life and can be difficult to treat. For patients with refractory epilepsy who are not candidates for resection, neurostimulation, the electrical modulation of epileptogenic brain tissue, is an emerging treatment alternative. Several forms of neurostimulation are currently available, and therapy selection hinges on relative efficacy for seizure control and amelioration of neuropsychiatric comorbidities. Here, we review the current evidence for how invasive and noninvasive neurostimulation therapies affect mood and cognition in persons with epilepsy. Invasive therapies include vagus nerve stimulation (VNS), deep brain stimulation (DBS), and responsive neurostimulation (RNS). Noninvasive therapies include trigeminal nerve stimulation (TNS), repetitive transcranial magnetic stimulation (rTMS), and transcranial direct current stimulation (tDCS). Overall, current evidence supports stable cognition and mood with all neurostimulation therapies, although there is some evidence that cognition and mood may improve with invasive forms of neurostimulation. More research is required to optimize the effects of neurostimulation for improvements in cognition and mood