Robert Boyle’s Religious Life, Attitudes, and Vocation

Robert Boyle is an outstanding example of a Christian scientist whose faith interacted fundamentally with his science. His remarkable piety was the driving force behind his interest in science and his Christian character shaped the ways in which he conducted his scientific life. A deep love for scripture, coupled ironically with a lifelong struggle with religious doubt, led him to write several important books relating scientific and religious knowledge. Ultimately, he was attracted to the mechanical philosophy because he thought it was theologically superior to traditional Aristotelian natural philosophy: by denying the existence of a quasi-divine ‘Nature’ that functioned as an intermediary between God and the world, it more clearly preserved God’s sovereignty and more powerfully motivated people to worship their creator

Creation, Contingency, and Early Modern Science: The Impact of Voluntarist Theology on Seventeenth-Century Natural Philosophy

Could God have made it true that 2 + 2 = 5? Was he bound to make the best of all possible worlds? Is he able at this moment to alter the course of nature, either in whole or in part? Questions like these are often associated with medieval theology, not with early modern science. But science is done by people, and people have not always practiced the rigorous separation of science and theology that has come to characterize the modern world. Although many 17th century scientists sought validity for their work apart from revelation, divorcing science from religion was something they never intended. Indeed most natural philosophers of the scientific revolution assumed without question that the world and the human mind had been created by God. This was no small admission, for it meant that both the manner in which and the degree to which the world could be understood depended upon how God had acted in creating it and how he continued to act in sustaining it. Fifty years ago the late British philosopher M.B. Foster identified two different theologies of creation which differ profoundly in their implications for natural science. Rationalist theology, which assigns to God the activity of pure reason, involves both a rationalist X philosophy of nature and a rationalist theory of knowledge of nature. Voluntarist theology, which attributes to God an activity of will not wholly determined by reason, implies that the products of his creative activity are contingent and can be known only empirically. By a careful analysis of four natural philosophies of the early modern period--those of Galileo, Descartes, Boyle, and Newton--! intend to show that there was indeed a connection between theological voluntarism and empirical science in the 17th century

The Belgrade and Sidney Register

A history of Belgrade, ME and Sidney, ME including: Early Settlers, Incorporation and Town Officers, Military Matters, Manufacturing Account, Church Account, Educational Notes, and General Survey of each town

CD155 on HIV-infected cells is not modulated by HIV-1 Vpu and Nef but synergizes with NKG2D ligands to trigger NK cell lysis of autologous primary HIV-infected cells

Activation of primary CD4(+) T cells induces the CD155, but not the CD112 ligands for the natural killer (NK) cell activation receptor (aNKR) CD226 [DNAX accessory molecule-1 (DNAM-1)]. We hypothesize that HIV productively infects activated CD4(+) T cells and makes itself vulnerable to NK cell-mediated lysis when CD155 on infected T cells engages DNAM-1. The primary objective of this study is to determine whether CD155 alone or together with NKG2D ligands triggers autologous NK cell lysis of HIV-infected T cells and whether HIV modulates CD155. To determine whether HIV modulates this activation ligand, we infected “activated” CD4(+) T cells with HIV in the absence or presence of Nef and/or Vpu and determined by flow cytometry whether they modulated CD155. To determine if CD155 alone, or together with NKG2D ligands, triggered NK cell lysis of autologous HIV-infected T cells, we treated purified NK cells with DNAM-1 and/or NKG2D blocking antibodies before the addition of purified autologous HIV-infected cells in cytolytic assays. Finally, we determined whether DNAM-1 works together with NKG2D as an NK cell coactivation receptor (caNKR) or whether they work independently as aNKRs to induce an NK cell lytic response. We demonstrate that HIV and specifically Nef and/or Vpu do not modulate CD155 on infected primary T cells; and both CD155 and NKG2D ligands synergize as aNKRs to trigger NK cell lysis of the infected cell

Multispectral satellite imaging improves detection of large individual fossils

Palaeontological field surveys in remote regions are a challenge, because of uncertainty in finding new specimens, high transportation costs, risks for the crew and a long time commitment. The effort can be facilitated by using high-resolution satellite imagery. Here we present a new opportunity to investigate remote fossil localities in detail, mapping the optical signature of individual fossils. We explain a practical workflow for detecting fossils using remote-sensing platforms and cluster algorithms. We tested the method within the Petrified Forest National Park, where fossil logs are sparse in a large area with mixed lithologies. We ran both unsupervised and supervised classifications, obtaining the best estimations for the presence of fossil logs using the likelihood and spectral angle mapper algorithms. We recognized general constraints and described logical and physical pros and cons of each estimated map. We also explained how the outcomes should be critically evaluated with consistent accuracy tests. Instead of searching for fossiliferous outcrops, our method targets single fossil specimens (or highly condensed accumulations), obtaining a significant increase in potential efficiency and effectiveness of field surveys. When repeatedly applied to the same region over time, it could also be useful for monitoring palaeontological heritage localities. Most importantly, the method here described is feasible, easily applicable to both fossil logs and bones, and represents a step towards standard best practices for applying remote sensing in the palaeontological field

Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays

Natural killer (NK) cells are a vital component of the innate immune response to virus-infected cells. It is important to understand the ability of NK cells to recognize and lyse HIV-1 infected cells because identifying any aberrancy in NK cell function against HIV-infected cells could potentially lead to therapies that would enhance their cytolytic activity. There is a need to use HIV-infected primary T-cell blasts as target cells rather then infected-T-cell lines in the cytotoxicity assays. T-cell lines, even without infection, are quite susceptible to NK cell lysis. Furthermore, it is necessary to use autologous primary cells to prevent major histocompatibility complex class I mismatches between the target and effector cell that will result in lysis. Early studies evaluating NK cell cytolytic responses to primary HIV-infected cells failed to show significant killing of the infected cells 1,2. However, using HIV-1 infected primary T-cells as target cells in NK cell functional assays has been difficult due the presence of contaminating uninfected cells 3. This inconsistent infected cell to uninfected cell ratio will result in variation in NK cell killing between samples that may not be due to variability in donor NK cell function. Thus, it would be beneficial to work with a purified infected cell population in order to standardize the effector to target cell ratios between experiments 3,4. Here we demonstrate the isolation of a highly purified population of HIV-1 infected cells by taking advantage of HIV-1's ability to down-modulate CD4 on infected cells and the availability of commercial kits to remove dead or dying cells 3-6. The purified infected primary T-cell blasts can then be used as targets in either a degranulation or cytotoxic assay with purified NK cells as the effector population 5-7. Use of NK cells as effectors in a degranulation assay evaluates the ability of an NK cell to release the lytic contents of specialized lysosomes 8 called "cytolytic granules". By staining with a fluorochrome conjugated antibody against CD107a, a lysosomal membrane protein that becomes expressed on the NK cell surface when the cytolytic granules fuse to the plasma membrane, we can determine what percentage of NK cells degranulate in response to target cell recognition. Alternatively, NK cell lytic activity can be evaluated in a cytotoxic assay that allows for the determination of the percentage of target cells lysed by release of 51Cr from within the target cell in the presence of NK cells