Experiencia peruana sobre el flujograma de diagnóstico del virus de inmunodeficiencia humana: Peruvian experience on the human immunodeficiency virus diagnostic flowchart

Mr Editor:The Joint United Nations Program on HIV/AIDS (UNAIDS) proposed as a goal that the countries reach 95-95-95 by the year 2030, in other words, that 95% of people living with HIV (PLH) are diagnosed and of these that 95% receive antiretroviral treatment (ART) and at least 95% have undetectable viral load or viral suppression.Sr. Editor:El Programa Conjunto de las Naciones Unidas sobre el VIH/Sida (ONUSIDA) propuso como meta que los países alcancen para el año 2030 el 95-95-95, es decir que un 95% de personas viviendo con VIH (PVV) estén diagnosticadas y de éstas que el 95% reciban tratamiento antirretroviral (TAR) y al menos el 95% tengan carga viral indetectable o supresión viral

Diagnostic performance of the indirect immunofluorescence test for the detection of antibodies against HTLV-1

The objective of the study was to determine the diagnostic yield of the indirect immunofluorescence (IFI) test for the detection of antibodies against HTLV-1. A diagnostic test evaluation study was performed. HTLV-1-infected MT2 cells and HTLV-1-uninfected K-562 cells were cultured; then these cells were impregnated and fixed in sheets for immunofluorescence and faced to Peruvian sera. A total of 155 sera (80 HTLV-1-positive sera and 75 sera positive for other diseases) from the Peruvian Instituto Nacional de Salud were used. In addition, the parameters of repeatability (intra-laboratory) and reproducibility (in laboratories of the Peruvian coast, mountains and jungle) of the test were evaluated. The IFI test detected the presence of antibodies against HTLV-1 reaching a sensitivity of 98.75% (95% CI: 95.69 – 100.00%), a specificity of 98.67% (95% CI: 95.40 – 100.00%) and the Kappa index was 0.975. There were no false positives or false negatives; however, one undetermined result and one non-specific result were obtained. The test showed 100% qualitative agreement when performing the repeatability and reproducibility. The results obtained are comparable to the reference test. Therefore, the IFI test had a good diagnostic performance and would be useful for the confirmation of HTLV-1